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Antihelminthic and antiangiogenic effects of zinc oxide nanoparticles on intestinal and muscular phases of trichinellosis

Published online by Cambridge University Press:  18 July 2023

S.E. Ashoush Open the ORCID record for S.E. Ashoush [Opens in a new window]  and
E.K. Soliman Open the ORCID record for E.K. Soliman [Opens in a new window]
S.E. Ashoush*
Affiliation:
Medical Parasitology Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt
E.K. Soliman
Affiliation:
Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt
*
Corresponding author: S.E. Ashoush; Email: shaimaaashoush@gmail.com
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Abstract

Trichinellosis is a worldwide zoonotic disease affecting a wide range of mammals, including humans. It has intestinal and muscular phases. The current work was done to experimentally evaluate the efficacy of zinc oxide nanoparticles (ZnO NPs) and their combination with albendazole on intestinal and muscular stages of Trichinella spiralis (T. spiralis) infection. We had five main groups of mice: Group 1, non-infected control; Group 2, infected control; Group 3, infected and treated with albendazole; Group 4, infected and treated with ZnO NPs; and Group 5, infected and treated with albendazole and ZnO NPs. Each group was divided into two subgroups (A for the intestinal phase and B for the muscular phase). Drug effects were evaluated by parasitological, histopathological, and biochemical studies, including oxidant/antioxidant analysis and vascular endothelial growth factor (VEGF) gene expression in muscle tissue by quantitative real-time PCR. ZnO NPs resulted in a significant reduction of both intestinal and muscular phases of T. spiralis. Their combination with albendazole resulted in the complete eradication of adult worms and the maximum reduction of larval deposition in muscle tissue. Additionally, the treatment showed improvement in T. spiralis-induced pathological changes and oxidative stress status. Moreover, a significant decrease in VEGF gene expression was detected in the treated groups when compared with the infected control. In conclusion, ZnO NPs presented an antihelminthic effect against both adult and larval stages of T. spiralis. In addition, it enhanced antioxidant status and suppressed angiogenesis in muscle.

Keywords

T. spiralisalbendazoleZnO NPsoxidative stressVEGF
Type
Research Paper
Information
Journal of Helminthology , Volume 97 , 2023 , e56
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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