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Serological Investigations with Loa loa Antigens

Published online by Cambridge University Press:  05 June 2009

E. O. Ogunba
E. O. Ogunba
Affiliation:
Department of Medical Microbiology, University College Hospital, University of Ibadan, Nigeria
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Extract

Antigens prepared from adult worms and microfilariae of Loa loa were used for an assessment of the sensitivity and specificity in diagnosis of double immuno-diffusion in agar gel, haemagglutination and fluorescent antibody tests.

Both antigens formed a broad band which moved slowly towards the cathode on electrophoresis, and each antigen separated into two protein fractions when passed through Sephadex G-200.

Both precipitating and non-precipitating antibodies were detected in sera from Loa loa patients. The haemagglutination test and fluorescent antibody test were sensitive in detecting Loa loa antibodies and would be of diagnostic value, but the double diffusion test in agar-gel was insensitive and would have a limited value in loiasis diagnosis.

Type
Research Article
Information
Journal of Helminthology , Volume 46 , Issue 3 , September 1972 , pp. 241 - 250
Copyright
Copyright © Cambridge University Press 1972

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