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The origin of the triploid in Paragonimus westermani on the basis of variable regions in the mitochondrial DNA

Published online by Cambridge University Press:  12 April 2024

T. Agatsuma*
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
M. Iwagami
Affiliation:
Department of Environmental Health Science, Kochi Medical School, Oko, Nankoku City, Kochi 783-8505, Japan
Y. Sato
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
J. Iwashita
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
S.J. Hong
Affiliation:
Department of Parasitology, Chung-Ang University College of Medicine, Heukseok-dong, Tongjak-gu, Seoul 156-756, Korea
S.Y. Kang
Affiliation:
Department of Parasitology, Chung-Ang University College of Medicine, Heukseok-dong, Tongjak-gu, Seoul 156-756, Korea
L.Y. Ho
Affiliation:
People's Hospital, Beijing Medical University, Beijing 100034, China
K.E. Su
Affiliation:
Department of Parasitology, College of Medicine, National Taiwan University, Taipei, Taiwan
K. Kawashima
Affiliation:
School of Health Sciences, Kyusyu University, Fukuoka 812-8582, Japan
T. Abe
Affiliation:
Department of Molecular Biology, Akita Prefectural University, Shimo Shinjo, Akita City, Akita 010-0195, Japan
*
*Fax: +81 88 880 2535 E-mail: agatsuma@med.kochi-ms.ac.jp

Abstract

Triploid, parthenogenetic forms of the lungfluke, Paragonimus westermani, occur in Japan, Korea and China. The origin(s) of triploidy has been debated over the years. Sequences of two regions in the mitochondrial DNA, i.e. partial lrRNA (16S), and a portion of the non-coding region, were obtained from natural populations of P. westermani. All triploid individuals (Japan, Korea, China) and a single tetraploid individual (China) had identical sequences in the 16S region studied. Some sequence variation was observed among diploids, with those from Taiwan being distinct from the remainder. Both neighbour joining and parsimony trees using the 16S region placed diploid individuals from southwestern Japan close to the triploids and the tetraploid. The fragment amplified from the mitochondrial non-coding region showed dimorphism. One form (type A) consisted of 239bp comprising two identical tracts of 70bp separated by a tract of 93bp. The second form (Type B) consisted of only a single 70bp tract. All diploid individuals from Taiwan, China and Korea possessed type A, while those from Japan were polymorphic; individuals from Oita and Hyogo had type B, those from Chiba had type A, but both types were found in Mie. On the other hand, all of the triploid individuals and two tetraploid individuals possessed type B. Both the form present in the non-coding region and the 16S sequence suggest an affinity between a south-eastern group of diploid populations in Japan and the triploid form. A possible mechanism responsible for the origin of the triploid is discussed.

Type
Review Article
Copyright
Copyright © Cambridge University Press 2003

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