Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-20T19:10:05.588Z Has data issue: false hasContentIssue false

Molecular characterization of veterinary important trematode and cestode species in the mithun Bos frontalis from north-east India

Published online by Cambridge University Press:  07 September 2015

J.K. Chamuah
Affiliation:
National Research Centre on Mithun, Jarnapani, Nagaland, India
O.K. Raina*
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
H. Lalrinkima
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
S.S. Jacob
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
M. Sankar
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India
A. Sakhrie
Affiliation:
National Research Centre on Mithun, Jarnapani, Nagaland, India
S. Lama
Affiliation:
National Research Centre on Mithun, Jarnapani, Nagaland, India
P.S. Banerjee
Affiliation:
Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, India

Abstract

Helminth infections in the mithun Bos frontalis, including the liver fluke Fasciola gigantica, hepato-gastric amphistomes Explanatum explanatum, Paramphistomum epiclitum and Calicophoron calicophorum, and the cestodes Echinococcus granulosus and E. ortleppi were studied in north-east India over a 2-year period from 2012 to 2014. Cystic echinococcosis caused by E. granulosus and E. ortleppi was found to be highly prevalent in the mithun, with E. ortleppi being reported for the first time. Molecular markers, including the internal transcribed spacer 2 (ITS-2), 28S rDNA and mitochondrial NADH dehydrogenase sub-unit1 (nad1) were used to confirm the identification of the trematode and cestode species.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bhattacharya, D., Bera, A.K., Bera, B.C., Maity, A. & Das, S.K. (2007) Genotypic characterization of Indian cattle, buffalo and sheep isolates of Echinococcus granulosus . Veterinary Parasitology 143, 371374.Google Scholar
Chakraborty, A. (2001) Pathology of Fasciola gigantica infection in captive wild herbivores with special reference to SEM and EDAX study. Indian Veterinary Journal 78, 4347.Google Scholar
Chamuah, J.K. (2005) Studies on some aspects of parasites of mithun (Bos frontalis). M.V.Sc . thesis submitted to Assam Agricultural University, Khanapara, Guwahati, Assam, India.Google Scholar
Chaurasia, R.K., Mech, A., Dhali, A., Vidyarthi, V.K., Rajkhowa, C. & Sharma, V.B. (2010) Effect of altitude and season on rectal temperature, pulse rate and respiration rate in mithun (Bos frontalis). Indian Journal of Hill Farming 23, 2529.Google Scholar
Hayashi, K., Ichikawa-Seki, M., Mohanta, U.K., Singh, T.S., Hiromu, T.S. & Itagaki, S.T. (2015) Molecular phylogenetic analysis of Fasciola flukes from eastern India. Parasitology International 64, 334338.Google Scholar
Huang, W.Y., He, B., Wang, C.R. & Zhu, X.Q. (2004) Characterization of Fasciola species from Mainland China by ITS-2 ribosomal DNA sequence. Veterinary Parasitology 120, 7583.Google Scholar
Ichikawa, M., Iwata, N. & Itagaki, T. (2010) DNA types of aspermic Fasciola species in Japan. Journal of Veterinary Medical Science 72, 13711374.Google Scholar
Ichikawa, M., Bawn, S., Maw, N.N., Htun, L.L., Thein, M., Gyi, A., Sunn, K., Katakura, K. & Itagaki, T. (2011) Characterization of Fasciola spp. in Myanmar on the basis of spermatogenesis status and nuclear and mitochondrial DNA markers. Parasitology International 60, 474479.Google Scholar
Itagaki, T., Kikawa, M., Terasaki, K., Shibahara, T. & Fukuda, K. (2005) Molecular characterization of parthenogenic Fasciola species in Korea on the basis of DNA sequences of ribosomal ITS1 and mitochondrial NDI gene. Journal of Veterinary Medical Science 67, 11151118.CrossRefGoogle ScholarPubMed
Marcilla, A., Bargues, M.D. & Mas-Coma, S.A. (2002) PCR-RFLP assay for the distinction between Fasciola hepatica and Fasciola gigantica . Molecular and Cellular Probes 16, 327333.Google Scholar
Mohanta, U.K., Ichikawa-Seki, M., Shoriki, T., Katakura, K. & Itagaki, T. (2014) Characteristics and molecular phylogeny of Fasciola flukes from Bangladesh, determined based on spermatogenesis and nuclear and mitochondrial DNA analysis. Parasitology Research 113, 24932501.Google Scholar
Pednekar, R.P., Gatne, M.L., Thompson, R.C. & Traub, R.J. (2009) Molecular and morphological characterisation of Echinococcus from food producing animals in India. Veterinary Parasitology 165, 5865.Google Scholar
Peng, M., Ichinomiya, M., Ohtori, M., Ichikawa, M., Shibahara, T. & Itagaki, T. (2009) Molecular characterization of Fasciola hepatica, Fasciola gigantica and aspermic Fasciola sp. in China based on nuclear and mitochondrial DNA. Parasitology Research 105, 809815.Google Scholar
Prasad, P.K., Tandon, V., Biswal, D.K., Goswami, L.M. & Chatterjee, A. (2009) Use of sequence motifs as barcodes and secondary structures of internal transcribed spacer 2 (ITS2, rDNA) for identification of the Indian liver fluke, Fasciola (Trematoda: Fasciolidae). Bioinformation 3, 314320.CrossRefGoogle ScholarPubMed
Prasad, P.K., Goswami, L.M., Tandon, V. & Chatterjee, A. (2011) PCR-based molecular characterization and in-silico analysis of food-borne trematode parasites Paragonimus westermani, Fasciolopsis buski and Fasciola gigantica from north-east India using ITS2 rDNA. Bioinformation 6, 6468.Google Scholar
Raina, O.K., Jacob, S.S., Sankar, M., Bhattacharya, D., Bandyopadyay, S., Varghese, A., Chamuah, J.K. & Lalrinkima, H. (2015) Genetic characterization of Fasciola gigantica from different geographical regions of India by ribosomal DNA markers. Journal of Parasitic Diseases 39, 2732.Google Scholar
Rajkhowa, S., Bujarbaruah, K.M., Rajkhowa, C. & Thong, K. (2003) Haemato-biochemical changes in mithun with gastrointestinal nematodiasis. Indian Veterinary Journal 80, 10641066.Google Scholar
Sharma, M., Fomda, B.A., Mazta, S., Sehgal, R., Singh, B.B. & Malla, N. (2013) Genetic diversity and population genetic structure analysis of Echinococcus granulosus sensu stricto complex based on mitochondrial DNA signature. PLOS One 8, e82904.Google Scholar
Sharma, R.L., Dhar, D.N. & Raina, O.K. (1989) Studies on the prevalence and laboratory transmission of fascioliasis in animals in the Kashmir valley. British Veterinary Journal 145, 5761.Google Scholar