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Fractionation of Fasciola hepatica tegument antigens and their application to the serodiagnosis of experimental fascioliasis by the enzyme-linked immunosorbent assay

Published online by Cambridge University Press:  05 June 2009

George V. Hillyer  and
Adelfa E. Serrano
George V. Hillyer
Affiliation:
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras P. R. 00931, USA
Adelfa E. Serrano
Affiliation:
Laboratory of Parasite Immunology, Department of Biology, University of Puerto Rico, Rio Piedras P. R. 00931, USA
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Abstract

A Fasciola hepatica tegument antigen preparation was obtained from intact adult worms by solubilization with a non-ionic detergent, Nonident P-40. This antigen preparation contains antigens useful for the serodiagnosis of infection with this parasite. However, the antigen preparation is inadequate for use in the enzyme-linked immunosorbent assay (ELISA). The present work demonstrates that fractionation by demulsification of this antigen preparation with ammonium sulphate results in a soluble aqueous phase which contains F. hepatica serodiagnostic antigens which can then be applied to the ELISA. This F. hepatica tegument antigen preparation when used m the ELISA can detect rabbit fascioliasis two weeks after infection, with antibody levels peaking by 10 to 12 weeks of infection.

Type
Research Article
Information
Journal of Helminthology , Volume 60 , Issue 3 , September 1986 , pp. 173 - 178
Copyright
Copyright © Cambridge University Press 1986

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References

REFERENCES

Biguet, J. A., Capron, A., & Tran Van Ky, P. (1962) Les antigenes de Fasciola hepatica. Etude électrophoretique et immunoélectrophorétique. Identification des fractions et comparison avec les antigenes correspondant à sept autres helminthes. Annales de Parasitologie Humaine et Compare, 37, 221–231.CrossRefGoogle Scholar
Helenious, A. & Simons, K. (1975) Solubilization of membranes by detergents. Bioquimica et Biophysica Acta, 415, 29–79.CrossRefGoogle Scholar
Hillyer, G. V. (1980) Isolation of Fasciola hepatica tegument antigens. Journal of Clinical Microbiology, 12, 695–699.CrossRefGoogle ScholarPubMed
Hillyer, G. V. & Gomez De Rios, I. (1979) The enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of schistosomiasis. American Journal of Tropical Medicine Hygiene, 28, 237–241.CrossRefGoogle ScholarPubMed
Hillyer, G. V. & Santiago De Weil, N. (1979) Use of immunologic techniques to detect chemotherapeutic success in infections with Fasciola hepatica. II. The enzyme-linked immunosorbent assay in infected rats and rabbits. Journal of Parasitology, 65, 680–684.CrossRefGoogle ScholarPubMed
Keleti, G. & Lederer, W. H. (1974) Handbook of Micromethods for the Biological Sciences, Van Nostrand Reinhold Co., New York.Google Scholar
Levine, D. M., Hillyer, G. V. & Flores, S. I. (1980) Comparison of counterelectrophoresis, the enzyme-linked immunosorbent assay, and Kato fecal examination for the diagnosis of fascioliasis in mice and rabbits. American Journal of Tropical Medicine and Hygiene, 29, 602–608.CrossRefGoogle Scholar
Santiago De Weil, N., Hillyer, G. V. & Pacheco, E. (1984) Isolation of Fasciola hepatica genus-specific antigens. International Journal for Parasitology, 14, 197–206.CrossRefGoogle ScholarPubMed
Sewell, M. M. H. (1964) The immunology of fascioliasis II. Qualitative studies on the precipitin reaction. Immunology, 7, 671–679.Google ScholarPubMed
Tailliez, R., Mangalo, R. & Korach, S. (1967) Isolement d'un antigène speécifique de la grande douve du foie (Fasciola hepatica L.). Comptes Rendus Acadimie des Sciences Series D, 265, 466–469.Google Scholar