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Antigen production by encysted muscle larvae of Trichinella spiralis

Published online by Cambridge University Press:  18 November 2009

D. I. Pritchard
D. I. Pritchard
Affiliation:
Experimental Parasitology Unit, Department of Zoology, University of Nottingham, University Park, Nottingham NG7 2RD, UK
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Abstract

The production of excretory-secretory antigens by encysted muscle larvae of Trichinella spiralis has been investigated immuno-histochemically using an antiserum raised by infection in rabbits and purified both before and after conjugation by ion-exchange chromatography. The specificity of the antibody for excretory-secretory products was demonstrated by the pattern of staining of live worms in vitro and the failure of the labelled antibody to stain dead, non-metabolizing worms. Using this labelled antibody, and unlabelled antibody in the immunoperoxidase system, the presence of parasite antigen-bearing cells in close proximity to encysted muscle larvae has been demonstrated. This is believed to be the first demonstration of antigen production by encysted muscle larvae in vivo. The implications of this observation to current concepts of immunity to Trichinella spiralis are discussed.

Type
Research Papers
Information
Journal of Helminthology , Volume 59 , Issue 1 , March 1985 , pp. 71 - 77
Copyright
Copyright © Cambridge University Press 1985

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