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Antibody responses in the wild vole, Clethrionomys rufocanus bedfordiae, naturally infected with Echinococcus multilocularis by Western blotting

Published online by Cambridge University Press:  05 June 2009

A. Ito ,
M. Nakao ,
M. Ito ,
T. Matsuzaki ,
M. Kamiya  and
H. Kutsumi
A. Ito
Affiliation:
Department of Parasitology, Gifu University School of Medicine, Gifu 500, Japan
M. Nakao
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
M. Ito
Affiliation:
Central Institute for Experimental Animals, Kawasaki 216, Japan
T. Matsuzaki
Affiliation:
National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo 187, Japan
M. Kamiya
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Hokkaido University Sapporo 060, Japan
H. Kutsumi
Affiliation:
Department of Parasitology, Asahikawa Medical College, Asahikawa 078, Japan
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Abstract

Antibody responses against crude antigens and the two (Em18 and Em16) epitopes of Echinococcus mulfilocularis were analysed by Western blotting using sera from the wild vole, Clethrionomys rufocanus bedfordiae (Crb), which were captured in Hokkaido, Japan and found to have been naturally infected with eggs, and compared with those in patients of AHD and mice experimentally infected with protoscoleces of this parasite. Antibody responses in the wild vole were demonstrated most clearly with anti-Crb-IgG antiserum but more faintly with anti-rat-IgG antiserum and poorly with anti-mouse-IgG or anti-human-IgG antisera or Protein G. Although only two serum samples of the wild vole found naturally infected were analysed, antibody responses against Em18 and Em16 in these voles appeared to be similar to those in AHD patients but differed from those in mice.

Type
Research Note
Information
Journal of Helminthology , Volume 68 , Issue 3 , September 1994 , pp. 267 - 269
Copyright
Copyright © Cambridge University Press 1994

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References

Craig, P.S., Deshan, L. & Zhaoxun, D. (1991) Hydatid disease in China. Parasitology Today 7, 46–50.Google Scholar
Craig, P.S., Deshan, L, Macpherson, C.N.L., Dazhong, S., Reynolds, D., Barnish, G., Gottstein, B. & Zhirong, W. (1992) A large focus of alveolar echinococcosis in central China. Lancet 340, 826–831.CrossRefGoogle ScholarPubMed
Ito, A., Nakao, M., Kutsumi, H., Lightowlers, M.W., Itoh, M. & Sato, S. (1993) Serodiagnosis of alveolar hydatid disease by Western blotting. Transactions of the Royal Society of Tropical Medicine and Hygiene 87, 170–172.CrossRefGoogle ScholarPubMed
Ito, A., Wang, X. & Liu, Y. (1993) Differential serodiagnosis of alveolar and cystic hydatid disease in the People's Republic of China. American Journal of Tropical Medicine and Hygiene 49, 208–213.CrossRefGoogle ScholarPubMed
Kariwa, H., Arikawa, J., Takashima, I. & Hashimoto, N. (1992) Development and application of protein G antibody assay for the detection of antibody to hantavirus. Journal of Virological Methods 37, 345–354.CrossRefGoogle ScholarPubMed
Lingelbach, K. & Hinz, E. (1984) Echinococcus multilocularis: identification of proteins including antibody formation in metacestode infection. Journal of Helminthology 58, 1–6.Google Scholar
Miyauchi, T., Sakui, M., Ishige, M., Fukumoto, S., Ueda, A., Ito, M. & Ohbayashi, M. (1984) A case of multilocularis echinococcosis in a horse. Japanese Journal of Veterinary Research 32, 171–173.Google Scholar
Ohbayashi, M., Rausch, R.L. & Fay, F.H. (1971) On the ecology and distribution of Echinococcus spp. (Cestoda: Taeniidae), and characteristics of their development in the intermediate host. Japanese Journal of Veterinary Research, 19, Suppl. 1–53.Google Scholar
Schantz, P.M., Gottstein, B., Ammann, R. & Lanier, A. (1991) Hydatid and the Arctic. Parasitology Today 7, 35–36.CrossRefGoogle Scholar