A separated turbulent boundary layer over a flat plate was investigated by direct numerical simulation of the incompressible Navier–Stokes equations. A suction-blowing velocity distribution was prescribed along the upper boundary of the computational domain to create an adverse-to-favourable pressure gradient that produces a closed separation bubble. The Reynolds number based on inlet free-stream velocity and momentum thickness is 300. Neither instantaneous detachment nor reattachment points are fixed in space but fluctuate significantly. The mean detachment and reattachment locations determined by three different definitions, i.e. (i) location of 50% forward flow fraction, (ii) mean dividing streamline (ψ=0), (iii) location of zero wall-shear stress (τw=0), are in good agreement. Instantaneous vorticity contours show that the turbulent structures emanating upstream of separation move upwards into the shear layer in the detachment region and then turn around the bubble. The locations of the maximum turbulence intensities as well as Reynolds shear stress occur in the middle of the shear layer. In the detached flow region, Reynolds shear stresses and their gradients are large away from the wall and thus the largest pressure fluctuations are in the middle of the shear layer. Iso-surfaces of negative pressure fluctuations which correspond to the core region of the vortices show that large-scale structures grow in the shear layer and agglomerate. They then impinge on the wall and subsequently convect downstream. The characteristic Strouhal number St=fδ*in/U0 associated with this motion ranges from 0.0025 to 0.01. The kinetic energy budget in the detachment region is very similar to that of a plane mixing layer.