Unger, RH, Clark, GO, Scherer, PE, Orci, L. Lipid homeostasis, lipotoxicity and the metabolic syndrome. Biochim Biophys Acta. 2010; 1801, 209–214.
Berg, JM, Tymoczko, JL, Stryer, L. Triacylglycerols are highly concentrated energy stores. In Biochemistry (ed. Georgia Lee Hadler), 5th edn, 2002; pp. 641–642. WH Freeman and Company: New York.
Watt, MJ, Hoy, AJ. Lipid metabolism in skeletal muscle: generation of adaptive and maladaptive intracellular signals for cellular function. Am J Physiol Endocrinol Metabol. 2012; 302, E1315–E1328.
Ameer, F, Scandiuzzi, L, Hasnain, S, Kalbacher, H, Zaidi, N. De novo lipogenesis in health and disease. Metabolism. 2014; 63, 895–902.
Rosen, ED, Spiegelman, BM. Adipocytes as regulators of energy balance and glucose homeostasis. Nature. 2006; 444, 847–853.
Ducharme, NA, Bickel, PE. Minireview: lipid droplets in lipogenesis and lipolysis. Endocrinology. 2008; 149, 942–949.
Fu, S, Watkins, SM, Hotamisligil, GS. The role of endoplasmic reticulum in hepatic lipid homeostasis and stress signaling. Cell Metab. 2012; 15, 623–634.
Fagone, P, Jackowski, S. Membrane phospholipid synthesis and endoplasmic reticulum function. J Lipid Res. 2009; 50, S311–S316.
Jarvie, E, Hauguel-de-Mouzon, S, Nelson, SM, et al. Lipotoxicity in obese pregnancy and its potential role in adverse pregnancy outcome and obesity in the offspring. Clin Sci. 2010; 119, 123–129.
Walther, TC, Farese, RV. The life of lipid droplets. Biochim Biophys Acta. 2009; 1791, 459–466.
Gustafson, B, Gogg, S, Hedjazifar, S, et al. Inflammation and impaired adipogenesis in hypertrophic obesity in man. Am J Physiol Endocrinol Metabol. 2009; 297, E999–E1003.
Barker, DJ. The developmental origins of well-being. Philos Trans R Soc Lond B Biol Sci. 2004; 359, 1359–1366.
Barker, DJ, Osmond, C, Golding, J, Kuh, D, Wadsworth, ME. Growth in utero, blood pressure in childhood and adult life, and mortality from cardiovascular disease. BMJ. 1989; 298, 564–567.
Barker, DJ, Winter, PD, Osmond, C, Margetts, B, Simmonds, SJ. Weight in infancy and death from ischaemic heart disease. Lancet. 1989; 2, 577–580.
Fall, CH, Sachdev, HS, Osmond, C, et al. Adult metabolic syndrome and impaired glucose tolerance are associated with different patterns of BMI gain during infancy: data from the New Delhi Birth Cohort. Diabet Care. 2008; 31, 2349–2356.
McMillen, IC, Robinson, JS. Developmental origins of the metabolic syndrome: prediction, plasticity, and programming. Physiol Rev. 2005; 85, 571–633.
McGillick, EV, Lock, MC, Orgeig, S, Morrison, JL. Maternal obesity mediated predisposition to respiratory complications at birth and in later life: understanding the implications of the obesogenic intrauterine environment. Paediatr Respir Rev. 2016; 21, 11–18.
18. Australian Institute of Health and Welfare (AIHW). Australia’s mothers and babies 2014-in brief. Perinatal Statistics Series No. 32; Cat No. PER87. 2016.
Martin, S, Parton, RG. Lipid droplets: a unified view of a dynamic organelle. Nat Rev Mol Cell Biol. 2006; 7, 373–378.
Hall, PF, Almahbobi, G. Roles of microfilaments and intermediate filaments in adrenal steroidogenesis. Microsc Res Tech. 1997; 36, 463–479.
Almahbobi, G, Williams, LJ, Han, X-G, Hall, PF. Binding of lipid droplets and mitochondria to intermediate filaments in rat Leydig cells. J Reprod Fertil. 1993; 98, 209–217.
Merry, B. Mitochondrial structure in the rat adrenal cortex. J Anat. 1975; 119(Pt 3), 611–618.
Herms, A, Bosch, M, Ariotti, N, et al. Cell-to-cell heterogeneity in lipid droplets suggests a mechanism to reduce lipotoxicity. Curr Biol. 2013; 23, 1489–1496.
Blanchette-Mackie, EJ, Dwyer, NK, Barber, T, et al. Perilipin is located on the surface layer of intracellular lipid droplets in adipocytes. J Lipid Res. 1995; 36, 1211–1226.
Bartz, R, Li, W-H, Venables, B, et al. Lipidomics reveals that adiposomes store ether lipids and mediate phospholipid traffic. J Lipid Res. 2007; 48, 837–847.
Zicha, J, Kuneš, J, Devynck, M-A. Abnormalities of membrane function and lipid metabolism in hypertension: a review. Am J Hypertens. 1999; 12, 315–331.
van Meer, G. Cellular lipidomics. EMBO J. 2005; 24, 3159–3165.
Borkman, M, Storlien, LH, Pan, DA, et al. The relation between insulin sensitivity and the fatty-acid composition of skeletal-muscle phospholipids. N Engl J Med. 1993; 328, 238–244.
Holland, WL, Summers, SA. Sphingolipids, insulin resistance, and metabolic disease: new insights from in vivo manipulation of sphingolipid metabolism. Endocr Rev. 2008; 29, 381–402.
Wenk, MR. The emerging field of lipidomics. Nat Rev Drug Discov. 2005; 4, 594–610.
Ginsberg, HN. Insulin resistance and cardiovascular disease. J Clin Invest. 2000; 106, 453–458.
Kotronen, A, Velagapudi, V, Yetukuri, L, et al. Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations. Diabetologia. 2009; 52, 684–690.
Elle, IC, Olsen, LCB, Pultz, D, Rødkær, SV, Færgeman, NJ. Something worth dyeing for: molecular tools for the dissection of lipid metabolism in Caenorhabditis elegans. FEBS Lett. 2010; 584, 2183–2193.
Seppänen-Laakso, T, Laakso, I, Hiltunen, R. Analysis of fatty acids by gas chromatography, and its relevance to research on health and nutrition. Anal Chim Acta. 2002; 465, 39–62.
Peterson, BL, Cummings, BS. A review of chromatographic methods for the assessment of phospholipids in biological samples. Biomed Chromatogr. 2006; 20, 227–243.
Lemaitre, RN, King, IB, Mozaffarian, D, et al. Plasma phospholipid trans fatty acids, fatal ischemic heart disease, and sudden cardiac death in older adults the cardiovascular health study. Circulation. 2006; 114, 209–215.
Rissanen, T, Voutilainen, S, Nyyssönen, K, Lakka, TA, Salonen, JT. Fish oil–derived fatty acids, docosahexaenoic acid and docosapentaenoic acid, and the risk of acute coronary events. Circulation. 2000; 102, 2677–2679.
Patel, PS, Sharp, SJ, Jansen, E, et al. Fatty acids measured in plasma and erythrocyte-membrane phospholipids and derived by food-frequency questionnaire and the risk of new-onset type 2 diabetes: a pilot study in the European Prospective Investigation into Cancer and Nutrition (EPIC)–Norfolk cohort. Am J Clin Nutr. 2010; 92, 1214–1222.
García-Fontana, B, Morales-Santana, S, Navarro, CD, et al. Metabolomic profile related to cardiovascular disease in patients with type 2 diabetes mellitus: a pilot study. Talanta. 2016; 148, 135–143.
Blanksby, SJ, Mitchell, TW. Advances in mass spectrometry for lipidomics. Ann Rev Anal Chem. 2010; 3, 433–465.
Brügger, B. Lipidomics: analysis of the lipid composition of cells and subcellular organelles by electrospray ionization mass spectrometry. Ann Rev Biochem. 2014; 83, 79–98.
Park, JY, Lee, SH, Shin, MJ, Hwang, GS. Alteration in metabolic signature and lipid metabolism in patients with angina pectoris and myocardial infarction. PloS One. 2015; 10, e0135228.
Furse, S, Egmond, MR, Killian, JA. Isolation of lipids from biological samples. Mol Membr Biol. 2015; 32, 55–64.
Li, M, Zhou, Z, Nie, H, Bai, Y, Liu, H. Recent advances of chromatography and mass spectrometry in lipidomics. Anal Bioanal Chem. 2011; 399, 243–249.
Alberici, RM, Simas, RC, Sanvido, GB, et al. Ambient mass spectrometry: bringing MS into the ‘real world’. Anal Bioanal Chem. 2010; 398, 265–294.
Annesley, TM. Ion suppression in mass spectrometry. Clin Chem. 2003; 49, 1041–1044.
Colas, R, Pruneta-Deloche, V, Guichardant, M, et al. Increased lipid peroxidation in LDL from type-2 diabetic patients. Lipids. 2010; 45, 723–731.
Ando, J, Kinoshita, M, Cui, J, et al. Sphingomyelin distribution in lipid rafts of artificial monolayer membranes visualized by Raman microscopy. Proc Natl Acad Sci U S A. 2015; 112, 4558–4563.
Daemen, S, van Zandvoort, MAMJ, Parekh, SH, Hesselink, MKC. Microscopy tools for the investigation of intracellular lipid storage and dynamics. Mol Metab. 2016; 5, 153–163.
Mehlem, A, Hagberg, CE, Muhl, L, Eriksson, U, Falkevall, A. Imaging of neutral lipids by oil red O for analyzing the metabolic status in health and disease. Nat Protoc. 2013; 8, 1149–1154.
Goodpaster, BH, He, J, Watkins, S, Kelley, DE. Skeletal muscle lipid content and insulin resistance: evidence for a paradox in endurance-trained athletes. J Clin Endocrinol Metabol. 2001; 86, 5755–5761.
Chiu, H-C, Kovacs, A, Ford, DA, et al. A novel mouse model of lipotoxic cardiomyopathy. J Clin Investig. 2001; 107, 813–822.
Fukumoto, S, Fujimoto, T. Deformation of lipid droplets in fixed samples. Histochem Cell Biol. 2002; 118, 423–428.
Stadtländer, CT. Scanning electron microscopy and transmission electron microscopy of mollicutes: challenges and opportunities. In Modern Research and Educational Topics in Microscopy (eds. Méndez-Vilas A, and Díaz J), 2007; pp. 122–131. Formatex; Badajoz, Spain.
de Jonge, N, Ross, FM. Electron microscopy of specimens in liquid. Nat Nanotechnol. 2011; 6, 695–704.
Thiam, AR, Farese, RV Jr, Walther, TC. The biophysics and cell biology of lipid droplets. Nat Rev Mol Cell Biol. 2013; 14, 775–786.
Binns, D, Januszewski, T, Chen, Y, et al. An intimate collaboration between peroxisomes and lipid bodies. J Cell Biol. 2006; 173, 719–731.
Belazi, D, Sole-Domenech, S, Johansson, B, Schalling, M, Sjovall, P. Chemical analysis of osmium tetroxide staining in adipose tissue using imaging ToF-SIMS. Histochem Cell Biol. 2009; 132, 105–115.
Orlova, EV, Sherman, MB, Chiu, W, et al. Three-dimensional structure of low density lipoproteins by electron cryomicroscopy. Proc Natl Acad Sci. 1999; 96, 8420–8425.
Kizilyaprak, C, Daraspe, J, Humbel, BM. Focused ion beam scanning electron microscopy in biology. J Microsc. 2014; 254, 109–114.
Schertel, A, Snaidero, N, Han, HM, et al. Cryo FIB-SEM: volume imaging of cellular ultrastructure in native frozen specimens. J Struct Biol. 2013; 184, 355–360.
Haider, M, Muller, H, Uhlemann, S, et al. Prerequisites for a Cc/Cs-corrected ultrahigh-resolution TEM. Ultramicroscopy. 2008; 108, 167–178.
Flannigan, DJ, Zewail, AH. 4D electron microscopy: principles and applications. Acc Chem Res. 2012; 45, 1828–1839.
Charan, S, Chien, FC, Singh, N, Kuo, CW, Chen, P. Development of lipid targeting Raman probes for in vivo imaging of Caenorhabditis elegans. Chemistry. 2011; 17, 5165–5170.
Evans, CL, Xie, XS. Coherent anti-Stokes Raman scattering microscopy: chemical imaging for biology and medicine. Ann Rev Anal Chem. 2008; 1, 883–909.
Carter, EA, Tam, KK, Armstrong, RS, Lay, PA. Vibrational spectroscopic mapping and imaging of tissues and cells. Biophys Rev. 2009; 1, 95–103.
Enejder, A, Brackmann, C, Axäng, C, Åkeson, M, Pilon, M. CARS microscopy for the monitoring of lipid storage in C. elegans. In Proceedings of Biomedical Optics (BiOS) 2008, International Society for Optics and Photonics, 2008, pp. 686012.
Czamara, K, Majzner, K, Pacia, M, et al. Raman spectroscopy of lipids: a review. J Raman Spectrosc. 2015; 46, 4–20.
Billecke, N, Bosma, M, Rock, W, et al. Perilipin 5 mediated lipid droplet remodelling revealed by coherent Raman imaging. Integr Biol. 2015; 7, 467–476.
Sztalryd, C, Xu, G, Dorward, H, et al. Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation. J Cell Biol. 2003; 161, 1093–1103.
Nan, X, Potma, EO, Xie, XS. Nonperturbative chemical imaging of organelle transport in living cells with coherent anti-Stokes Raman scattering microscopy. Biophys J. 2006; 91, 728–735.
Song, YS, Won, YJ, Kim, DY. Time-lapse in situ fluorescence lifetime imaging of lipid droplets in differentiating 3T3-L1 preadipocytes with Nile Red. Curr Appl Phys. 2015; 15, 1634–1640.
Amaya, KR, Monroe, EB, Sweedler, JV, Clayton, DF. Lipid imaging in the zebra finch brain with secondary ion mass spectrometry. Int J Mass Spectrom. 2007; 260, 121–127.
Colliver, TL, Brummel, CL, Pacholski, ML, et al. Atomic and molecular imaging at the single-cell level with TOF-SIMS. Anal Chem. 1997; 69, 2225–2231.
Chen, R, Hui, L, Sturm, RM, Li, L. Three dimensional mapping of neuropeptides and lipids in crustacean brain by mass spectral imaging. J Am Soc Mass Spectrom. 2009; 20, 1068–1077.
Chen, Y, Allegood, J, Liu, Y, et al. Imaging MALDI mass spectrometry using an oscillating capillary nebulizer matrix coating system and its application to analysis of lipids in brain from a mouse model of Tay-Sachs/Sandhoff disease. Anal Chem. 2008; 80, 2780–2788.
Kner, P, Sedat, JW, Agard, DA, Kam, Z. High-resolution wide-field microscopy with adaptive optics for spherical aberration correction and motionless focusing. J Microsc. 2010; 237, 136–147.
Huang, B, Bates, M, Zhuang, X. Super-resolution fluorescence microscopy. Ann Rev Biochem. 2009; 78, 993–1016.
Huang, B, Wang, W, Bates, M, Zhuang, X. Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science. 2008; 319, 810–813.
Schmidt, R, Wurm, CA, Jakobs, S, et al. Spherical nanosized focal spot unravels the interior of cells. Nat Methods.. 2008; 5, 539–544.
Stehbens, S, Pemble, H, Murrow, L, Wittmann, T. Imaging intracellular protein dynamics by spinning disk confocal microscopy. Methods Enzymol. 2012; 504, 293–313.
Li, S, Hu, P, Malmstadt, N. Confocal imaging to quantify passive transport across biomimetic lipid membranes. Anal Chem. 2010; 82, 7766–7771.
Santi, PA. Light sheet fluorescence microscopy: a review. J Histochem Cytochem. 2011; 59, 129–138.
Girstmair, J, Zakrzewski, A, Lapraz, F, et al. Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development. BMC Dev Biol. 2016; 16, 22.
Royer, LA, Lemon, WC, Chhetri, RK, et al. Adaptive light-sheet microscopy for long-term, high-resolution imaging in living organisms. Nat Biotechnol. 2016; 34, 1267–1278.
Greenspan, P, Mayer, EP, Fowler, SD. Nile red: a selective fluorescent stain for intracellular lipid droplets. J Cell Biol. 1985; 100, 965–973.
Singh, R, Kaushik, S, Wang, Y, et al. Autophagy regulates lipid metabolism. Nature. 2009; 458, 1131–1135.
Sinha, RA, You, S-H, Zhou, J, et al. Thyroid hormone stimulates hepatic lipid catabolism via activation of autophagy. J Clin Invest. 2012; 122, 2428–2438.
Shibata, M, Yoshimura, K, Furuya, N, et al. The MAP1-LC3 conjugation system is involved in lipid droplet formation. Biochem Biophys Res Commun. 2009; 382, 419–423.
Koga, H, Kaushik, S, Cuervo, AM. Altered lipid content inhibits autophagic vesicular fusion. FASEB J. 2010; 24, 3052–3065.
Hölttä‐Vuori, M, Uronen, RL, Repakova, J, et al. BODIPY‐Cholesterol: a new tool to visualize sterol trafficking in living cells and organisms. Traffic. 2008; 9, 1839–1849.
Ishitsuka, R, Sato, SB, Kobayashi, T. Imaging lipid rafts. J Biochem. 2005; 137, 249–254.
O’Rourke, EJ, Soukas, AA, Carr, CE, Ruvkun, G. C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab. 2009; 10, 430–435.
Bader, C, Carter, E, Safitri, A, et al. Unprecedented staining of polar lipids by a luminescent rhenium complex revealed by FTIR microspectroscopy in adipocytes. Mol Biosyst. 2016; 12, 2064–2068.
Bader, CA, Brooks, RD, Ng, YS, et al. Modulation of the organelle specificity in Re(i) tetrazolato complexes leads to labeling of lipid droplets. RSC Adv. 2014; 4, 16345–16351.
Bader, CA, Shandala, T, Carter, EA, et al. A molecular probe for the detection of polar lipids in live cells. PloS One. 2016; 11, E0161557.
Bader, CA, Sorvina, A, Simpson, PV, et al. Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time. FEBS Lett. 2016; 590, 3051–3060.
Lo, K, Choi, A, Law, W. Applications of luminescent inorganic and organometallic transition metal complexes as biomolecular and cellular probes. Dalton Trans. 2012; 41, 6021–6047.
Svoboda, K, Yasuda, R. Principles of two-photon excitation microscopy and its applications to neuroscience. Neuron. 2006; 50, 823–839.
Hackett, MJ, McQuillan, JA, El-Assaad, F, et al. Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis. Analyst. 2011; 136, 2941–2952.