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Nutrient and hormone composition of milk is altered in rodent dams post-bariatric surgery

Published online by Cambridge University Press:  09 August 2019

Evangeline M. Deer
Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, MS, USA
Bradley Welch
Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, MS, USA
Laura L. Hernandez
Department of Dairy Science, University of Wisconsin-Madison, Madison, WI, USA
Randy J. Seeley
Department of Surgery, University of Michigan, Ann Arbor, MI, USA
Bernadette E. Grayson*
Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, MS, USA
Address for correspondence: Bernadette E. Grayson, Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, 2500 North State Street, Jackson, MS 39216, USA. Email:; Phone: 601-984-6809; Fax: 601-984-1655


Although bariatric surgery is approved for a woman of child-bearing age with an interest in subsequent pregnancy, reports of in utero growth issues during pregnancy have garnered a closer look at the impact of maternal surgical weight loss on the pre- and postpartum periods. Offspring of dams having received vertical sleeve gastrectomy (VSG) are born small-for-gestational age and have increased risk for metabolic syndrome later in life. Here, we aimed to determine whether the postnatal catch-up growth trajectory of bariatric offspring may be affected by milk composition. Milk samples were collected at postnatal day 15/16 from dams having received VSG surgery and fed a high-fat diet (HFD) (H-VSG), Sham surgery and fed chow (C-Sham), or Sham surgery and fed HFD (H-Sham). Milk obtained from H-VSG dams had elevated glucose (P < 0.05) and significantly reduced triglyceride content (P < 0.01). Milk from H-Sham dams had the lowest amount of milk protein (P < 0.05). Fatty acid composition measured by fractionation was largely not affected by surgery but rather maternal diet. No difference was observed in milk leptin levels; however, insulin, adiponectin, and growth hormone levels were significantly increased in milk from H-VSG animals. H-Sham had the lowest level of immunoglobulin (Ig)A, whereas IgG was significantly reduced in H-VSG. Taken together, the quality of milk from H-VSG dams suggests that milk composition could be a factor in reducing the rate of growth during the lactation period.

Original Article
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2019 

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Colquitt, JL, Pickett, K, Loveman, E, Frampton, GK. Surgery for weight loss in adults. Cochrane Database Syst Rev. 2014; 8, Cd003641. doi: 10.1002/14651858.CD003641.pub4.Google Scholar
Himpens, J, Dobbeleir, J, Peeters, G. Long-term results of laparoscopic sleeve gastrectomy for obesity. Ann Surg. 2010; 252, 319324.CrossRefGoogle ScholarPubMed
Peterli, R, Wölnerhanssen, B, Peters, T, et al. Improvement in glucose metabolism after bariatric surgery: comparison of laparoscopic Roux-en-Y gastric bypass and laparoscopic sleeve gastrectomy: a prospective randomized trial. Ann Surg. 2009: 250, 234241.CrossRefGoogle ScholarPubMed
Chambers, AP, Jessen, L, Ryan, KK, et al. Weight-independent changes in blood glucose homeostasis after gastric bypass or vertical sleeve gastrectomy in rats. Gastroenterology. 2011: 141, 950958.CrossRefGoogle ScholarPubMed
Schauer, PR, Kashyap, SR, Wolski, K, et al. Bariatric surgery versus intensive medical therapy in obese patients with diabetes. N Engl J Med. 2012: 366, 15671576.CrossRefGoogle ScholarPubMed
Stemmer, K, Bielohuby, M, Grayson, BE, et al. Roux-en-Y gastric bypass surgery but not vertical sleeve gastrectomy decreases bone mass in male rats. Endocrinology. 2013: 154, 20152024.CrossRefGoogle Scholar
Grayson, BE, Schneider, KM, Woods, SC, Seeley, RJ. Improved rodent maternal metabolism but reduced intrauterine growth after vertical sleeve gastrectomy. Sci Transl Med. 2013: 5, 199ra112. doi: 10.1126/scitranslmed.3006505.CrossRefGoogle ScholarPubMed
Teitelman, M, Grotegut, C, Williams, N, Lewis, J. The impact of bariatric surgery on menstrual patterns. Obes Surg. 2006: 16, 1457.CrossRefGoogle ScholarPubMed
Rochester, D, Jain, A, Polotsky, AJ, et al. Partial recovery of luteal function after bariatric surgery in obese women. Fertil Steril. 2009: 92, 1410.CrossRefGoogle ScholarPubMed
Tan, O, Carr, BR. The impact of bariatric surgery on obesity-related infertility and in vitro fertilization outcomes. Semin Reprod Med. 2012: 30, 517528. doi: 10.1055/s-0032-1328880.CrossRefGoogle ScholarPubMed
Johansson, K, Cnattingius, S, Näslund, I, et al. Outcomes of pregnancy after bariatric surgery. N Engl J Med. 2015: 372, 814824. doi: 10.1056/NEJMoa1405789.CrossRefGoogle ScholarPubMed
Galazis, N, Docheva, N, Simillis, C, Nicolaides, KH. Maternal and neonatal outcomes in women undergoing bariatric surgery: a systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol. 2014: 181, 4553. doi: 10.1016/j.ejogrb.2014.07.015.CrossRefGoogle ScholarPubMed
Parker, MH, Berghella, V, Nijjar, JB. Bariatric surgery and associated adverse pregnancy outcomes among obese women. J Maternal-fetal Neonatal Med. 2015: 29, 17471750. doi: 10.3109/14767058.2015.1060214.CrossRefGoogle ScholarPubMed
Baker, RW, Osman, J, Bodnar, RJ. Differential actions of dopamine receptor antagonism in rats upon food intake elicited by either mercaptoacetate or exposure to a palatable high-fat diet. Pharmacol Biochem Behav. 2001: 69, 201208.CrossRefGoogle ScholarPubMed
Gonzalez, I, Rubio, MA, Cordido, F, et al. Maternal and perinatal outcomes after bariatric surgery: a Spanish multicenter study. Obes Surg. 2015: 25, 436442. doi: 10.1007/s11695-014-1387-7.CrossRefGoogle ScholarPubMed
Stefater, M, Pérez–Tilve, D, Chambers, AP, et al. Sleeve gastrectomy induces loss of weight and fat mass in obese rats, but does not affect leptin sensitivity. Gastroenterology. 2010: 138, 24262436 e2423.CrossRefGoogle Scholar
Wilson-Perez, HE, Chambers, AP, Sandoval, DA, Stefater, MA, Woods, SC, Benoit, SC, Seeley, RJ. The effect of vertical sleeve gastrectomy on food choice in rats. Int J Obesit. 2013: 37, 288295.CrossRefGoogle ScholarPubMed
Parlee, SD, MacDougald, OA. Maternal nutrition and risk of obesity in offspring: the Trojan horse of developmental plasticity. Biochim Biophys Acta (BBA)-Mol Basis Dis. 2014: 1842, 495506.CrossRefGoogle ScholarPubMed
Lactation, I. o. M. U. C. o. N. S. D. P. a. Nutrition During Lactation. Washington (DC): National Academies Press (US) Milk Volume 5 (1991).Google Scholar
Ameye, L, Devlieger, R, De Preter, V, et al. Bariatric surgery does not appear to affect women’s breast-milk composition. J Nutr. 2018: 148, 10961102. doi: 10.1093/jn/nxy085.Google Scholar
Garretto, D, Kim, YK, Quadro, L, et al. Vitamin A and beta-carotene in pregnant and breastfeeding post-bariatric women in an urban population. J Perinatal Med. 2019: 47, 183189. doi: 10.1515/jpm-2018-0142.CrossRefGoogle Scholar
Ballard, O, Morrow, AL. Human milk composition: nutrients and bioactive factors. Pediatr Clin. 2013: 60, 4974.Google ScholarPubMed
Lönnerdal, B. Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr. 2003: 77, 1537S1543S.CrossRefGoogle ScholarPubMed
Bachour, P, Yafawi, R, Jaber, F, Choueiri, E, Abdel-Razzak, Z. Effects of Smoking, Mother’s age, body mass index, and parity number on lipid, protein, and secretory immunoglobulin A concentrations of human milk. Breastfeed Med. 2011: 7, 179188. doi: 10.1089/bfm.2011.0038.CrossRefGoogle ScholarPubMed
Rolls, BA, Gurr, MI, Van Duijvenvoorde, PM, Rolls, BJ, Rowe, EA. Lactation in lean and obese rats: effect of cafeteria feeding and of dietary obesity on milk composition. Physiol Behav. 1986: 38, 185190. doi: 10.1016/0031-9384(86)90153-8.CrossRefGoogle ScholarPubMed
Grayson, BE, Gutierrez-Aguilar, R, Sorrell, JE, et al. Bariatric surgery emphasizes biological sex differences in rodent hepatic lipid handling. Biol Sex differences. 2017: 8, 4. doi: 10.1186/s13293-017-0126-x.CrossRefGoogle ScholarPubMed
Fields, DA, Demerath, EW. Relationship of insulin, glucose, leptin, IL-6 and TNF-alpha in human breast milk with infant growth and body composition. Pediatr Obesit. 2012: 7, 304312. doi: 10.1111/j.2047-6310.2012.00059.x.CrossRefGoogle ScholarPubMed
Fields, DA, George, B, Williams, M, et al. Associations between human breast milk hormones and adipocytokines and infant growth and body composition in the first 6 months of life. Pediatr Obesit. 2017: 12 Suppl 1, 7885. doi: 10.1111/ijpo.12182.CrossRefGoogle ScholarPubMed
Miralles, O, Sánchez, J, Palou, A, Picó, C. A physiological role of breast milk leptin in body weight control in developing infants. Obesity. 2006: 14, 13711377. doi: 10.1038/oby.2006.155.CrossRefGoogle ScholarPubMed
Uysal, FK, Önal, EE, Aral, YZ, Adam, B, Dilmen, U, Ardicolu, Y. Breast milk leptin: its relationship to maternal and infant adiposity. Clin Nutr. 2002: 21, 157160. doi: 10.1054/clnu.2001.0525.CrossRefGoogle ScholarPubMed
Khodabakhshi, A, Ghayour-Mobarhan, M, Rooki, H, et al. Comparative measurement of ghrelin, leptin, adiponectin, EGF and IGF-1 in breast milk of mothers with overweight/obese and normal-weight infants. Eur J Clin Nutr. 2015: 69, 614618. doi: 10.1038/ejcn.2014.205.CrossRefGoogle ScholarPubMed
Goropashnaya, AV, Herron, J, Sexton, M, et al. Relationships between plasma adiponectin and body fat distribution, insulin sensitivity, and plasma lipoproteins in Alaskan Yup’ik Eskimos: the Center for Alaska Native Health Research study. Metab: Clin Exp. 2009: 58, 2229.CrossRefGoogle ScholarPubMed
Woo, JG, Guerrero, ML, Altaye, M, et al. Human milk adiponectin is associated with infant growth in two independent cohorts. Breastfeed Med: Off J Acad Breastfeed Med. 2009: 4, 101109. doi: 10.1089/bfm.2008.0137.CrossRefGoogle ScholarPubMed
Spann, RA, Lawson, WJ, Bidwell, GL, et al. Rodent vertical sleeve gastrectomy alters maternal immune health and fetoplacental development. Clin Sci (Lond). 2018: 132, 295312. doi: 10.1042/CS20171416.CrossRefGoogle ScholarPubMed
Weyermann, M, Brenner, H, Rothenbacher, D. Adipokines in human milk and risk of overweight in early childhood: a prospective cohort study. Epidemiology. 2007: 18, 722729. doi: 10.1097/EDE.0b013e3181567ed4.CrossRefGoogle ScholarPubMed
Fujimori, M, França, EL, Fiorin, V, et al. Changes in the biochemical and immunological components of serum and colostrum of overweight and obese mothers. BMC Pregnancy Childb. 2015: 15, 166. doi: 10.1186/s12884-015-0574-4.CrossRefGoogle ScholarPubMed