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Variation in the yak lipin-1 gene and its association with milk traits

  • Xin Wang (a1) (a2), Huitong Zhou (a1) (a2) (a3), Jon G. H. Hickford (a2) (a3), Shaobin Li (a1) (a2), Jiqing Wang (a1) (a2), Xiu Liu (a1) (a2), Jiang Hu (a1) (a2) and Yuzhu Luo (a1) (a2)...

Abstract

The aim of this research was to identify variation in the yak lipin-1 gene (LPIN1) and determine whether this variation affects milk traits. PCR-single stranded conformational polymorphism (PCR-SSCP) analysis was used to detect variation in the 5′ untranslated region of LPIN1 in 500 yaks from four populations: Tianzhu white yaks, Qinghai yaks, wild × domestic-cross yaks and Gannan yaks. Four unique PCR-SSCP patterns, representing four different DNA sequence variants (named A, B, C and D), were observed. These contained six single nucleotide polymorphisms. Female Gannan yaks with BC genotype produced milk with a higher fat content (P < 0.001) and total milk solids (P < 0.001), than those with the AA, AB and BB genotypes. These results would suggest that LPIN1 is having an effect on yak milk fat synthesis.

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Corresponding author

Authors for correspondence: Jiang Hu, Email huj@gsau.edu.cn and Yuzhu Luo, luoyz@gsau.edu.cn

References

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Bionaz, M and Loor, JJ (2008) ACSL1, AGPAT6, FABP3, LPIN1, and SLC27A6 are the most abundant isoforms in bovine mammary tissue and their expression is affected by stage of lactation. Journal of Nutrition 138, 10191024.
Byun, SO, Fang, Q, Zhou, H and Hickford, JGH (2009) An effective method for silver-staining DNA in large numbers of polyacrylamide gels. Analytical Biochemistry 385, 174175.
Cecchinato, A, Ribeca, C, Chessa, S, Cipolat-Gotet, C, Maretto, F, Casellas, J and Bittante, G (2014) Candidate gene association analysis for milk yield, composition, urea nitrogen and somatic cell scores in Brown Swiss cows. Animal: An International Journal of Animal Bioscience 8, 10621070.
Csaki, LS and Reue, K (2010) Lipins: multifunctional lipid metabolism proteins. Annual Review of Nutrition 30, 257272.
Gong, H, Zhou, H and Hickford, JGH (2011) Diversity of the glycine/tyrosine-rich keratin-associated protein 6 gene (KAP6) family in sheep. Molecular Biology Reports 38, 3135.
He, XP, Xu, XW, Zhao, SH, Fan, B, Yu, M, Zhu, MJ, Li, CC, Peng, ZZ and Liu, B (2009) Investigation of LPIN1 as a candidate gene for fat deposition in pigs. Molecular Biology Reports 36, 11751180.
Mele, M, Conte, G, Castiglioni, B, Chessa, S, Macciotta, NP, Serra, A, Buccioni, A, Pagnacco, G and Secchiari, P (2007) Stearoylcoenzyme A desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. Journal of Dairy Science 90, 44584465.
Nafikov, RA, Schoonmaker, JP, Korn, KT, Noack, K, Garrick, DJ, Koehler, KJ, Minick-Bormann, J, Reecy, JM, Spurlock, DE and Beitz, DC (2014) Polymorphisms in lipogenic genes and milk fatty acid composition in Holstein dairy cattle. Genomics 104, 572581.
Nikkhah, A (2011) Science of camel and yak milks: human nutrition and health perspectives. Food and Nutrition Sciences 2, 667673.
Pegolo, S, Cecchinato, A, Mele, M, Conte, G, Schiavon, S and Bittante, G (2016) Effects of candidate gene polymorphisms on the detailed fatty acids profile determined by gas chromatography in bovine milk. Journal of Dairy Science 99, 45584573.
Schaller, GB and Liu, W (1996) Distribution, status and conservation of wild yak Bos grunniens. Biological Conservation 76, 18.
Zhou, H, Hickford, JG and Fang, Q (2006) A two-step procedure for extracting genomic DNA from dried blood spots on filter paper for polymerase chain reaction amplification. Analytical Biochemistry 354, 159161.

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Variation in the yak lipin-1 gene and its association with milk traits

  • Xin Wang (a1) (a2), Huitong Zhou (a1) (a2) (a3), Jon G. H. Hickford (a2) (a3), Shaobin Li (a1) (a2), Jiqing Wang (a1) (a2), Xiu Liu (a1) (a2), Jiang Hu (a1) (a2) and Yuzhu Luo (a1) (a2)...

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