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Transduction in Streptococcus lactis

Published online by Cambridge University Press:  01 June 2009

Lois K. Allen ,
W. E. Sandine  and
P. R. Elliker
Lois K. Allen
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
W. E. Sandine
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
P. R. Elliker
Affiliation:
Oregon Agricultural Experiment Station, Corvallis, Oregon, U. S. A.*
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Summary

Transduction experiments were carried out using recipient strain Streptococcus lactis C2Ss (streptomycin-sensitive) and virulent phages prepared on donor strain Str. lactis C2Sr (streptomycin-resistant). At a high multiplicity of infection of 10, transduction to streptomycin resistance occurred at a frequency approximately 1 in 30 surviving cells and the recombinant character exhibited a delayed expression. Transduction did not occur when phages propagated on Str. lactis C2Ss were used or when phage antiserum was added to the transducing phage suspension. The transducing phage manifested an altered lytic cycle following passage through the Str. lactis C2Sr donor which rendered it incapable of reinfecting the donor but did not alter its virulence for the Str. lactis C2Ss recipient.

Type
Original Articles
Information
Journal of Dairy Research , Volume 30 , Issue 3 , October 1963 , pp. 351 - 357
Copyright
Copyright © Proprietors of Journal of Dairy Research 1963

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References

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