Skip to main content Accessibility help
×
Home

Toll-like receptor 4 gene polymorphisms influence milk production traits in Chinese Holstein cows

  • Mengqi Wang (a1), Hailiang Song (a2), Xiaorui Zhu (a1), Shiyu Xing (a1), Meirong Zhang (a1), Huimin Zhang (a1), Xiaolong Wang (a1), Zhangping Yang (a1), Xiangdong Ding (a2), Niel A. Karrow (a3), Sven König (a4) and Yongjiang Mao (a1) (a3) (a5)...

Abstract

The research reported in this Research Communication aimed to describe the influence of Toll-like receptor 4 gene polymorphisms on milk production traits in Chinese Holstein cows. Toll-like receptor 4 (TLR4) is an important member of the toll-like receptor gene family that is widely found in various organisms. Since TLR4 can identify molecular patterns from various pathogenic microorganisms and induce natural and acquired immunity, it plays an important role in disease resistance in dairy cows. Two single nucleotide polymorphisms (SNPs) of TLR4 (c.−226 G > C and c.2021 C > T) that were previously found to be associated with health traits were genotyped using Sequenom MassARRAY (Sequenom Inc., San Diego, CA) for Chinese Holstein cows (n = 866). The associations between SNPs or their haplotypes and milk production traits and somatic cell count were analyzed by the generalized linear model procedure of Statistics Analysis System software (SAS). The c.−226 G > C and c.2021 C > T showed low linkage disequilibrium (r2 = 0·192). There was no association between these two SNPs and SCC, but significant effects were found for SNP c.−226 G > C on test-day milk yield, fat content, protein content, and total solid and milk urea nitrogen (P < 0·05), and SNP c.2021 C > T and the SNP haplotypes on test-day milk yield, fat content, protein content, lactose content and total solids (P < 0·05). The software MatInspector revealed that c.−226 G > C was located within several potential transcription factor binding sites, including transcription factor AP-2. The polymorphisms c.−226 G > C and c.2021 C > T had significant effects on the milk production for Chinese Holstein, and these SNP could be used for molecular marker-assisted selection of milk production.

Copyright

Corresponding author

*For correspondence; e-mail: cattle@yzu.edu.cn

Footnotes

Hide All

These authors contributed equally to this work.

Footnotes

References

Hide All
Beecher, C, Daly, M, Childs, S, Berry, DP, Magee, DA, McCarthy, TV & Giblin, L 2010 Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genetics 11 99
Beecher, C, Daly, M, Ross, RP, Flynn, J, McCarthy, TV & Giblin, L 2012 Characterization of the bovine innate immune response in milk somatic cells following intramammary infection with Streptococcus dysgalactiae subspecies dysgalactiae. Journal of Dairy Science 95 57205729
Cartharius, K, Frech, K, Grote, K, Klocke, B, Haltmeier, M, Klingenhoff, A, Frisch, M, Bayerlein, M & Werner, T 2005 Matinspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics 21 29332942
Hilger-Eversheim, K, Moser, M, Schorle, H & Buettner, R 2000 Regulatory roles of AP-2 transcription factors in vertebrate development, apoptosis and cell-cycle control. Gene 260 112
Hill, WG & Robertson, A 1968 Linkage disequilibrium in finite populations. Theoretical & Applied Genetics 38 226231
Mishra, C, Kumar, S, Yathish, HM, Choudhary, R & Kumar, A 2016. Association of tlr4 gene variants with milk production traits in crossbred cattle. Agri Gene 1 6971.
Miyake, K 2007 Innate immune sensing of pathogens and danger signals by cell surface Toll-like receptors. Seminars in Immunology 19 310
Ruiz-Larrañaga, O, Manzano, C, Iriondo, M, Garrido, JM, Molina, E, Vazquez, P, Juste, RA & Estonba, A 2011 Genetic variation of toll-like receptor genes and infection by Mycobacterium avium ssp. paratuberculosis in Holstein-Friesian cattle. Journal of Dairy Science 94 36353641
Rupp, R & Boichard, D 1999 Genetic parameters for clinical mastitis, somatic cell score, production, udder type and milking easy in first lactation Holsteins. Journal of Dairy Science 82 21982204
Sharma, BS, Mount, J & Karrow, NA 2008 Functional characterization of a single nucleotide polymorphism in the 5′ UTR region of the bovine toll-like receptor 4 gene. Developmental Biology 132 331336
Sharma, BS, Abo-Ismail, MK, Schenkel, FS, You, Q, Verschoor, CP, Pant, SD, Karrow, NA 2015 Association of TLR4 polymorphisms with Mycobacterium avium subspecies paratuberculosis infection status in Canadian Holsteins. Animal Genetics 46 560565
Shi, YY & He, L 2005 SHEsis, a powerful software platform for analyses of linkage disequilibrium, haplotype construction & genetics association at polymorphism loci. Cell Research 15 9798
Stephens, M, Smith, N & Donnelly, P 2001 A new statistical method for haplotype reconstruction from population data. American Journal of Human Genetics 68 978989
Tian, W, Wu, T, Zhao, R, Xu, J, He, Y, & Wang, H 2017 Responses of milk production of dairy cows to jugular infusions of a mixture of essential amino acids with or without exclusion leucine or arginine. Animal Nutrition 3 271275
Wang, WP, Xu, SZ, Gao, X, Li, JY, Ren, HY & Zhuo-ma, LR 2008 Cloning and SNP screening of the TLR4 gene and the association between its polymorphism and somatic cell score in dairy cattle. South African Journal of Animal Science 38 101109
Wang, XP, Luoreng, ZM, Gao, SX, Guo, DS, Li, JY, Gao, X, Xu, SZ, Li, F, Chen, G & Wang, JR 2014 Haplotype analysis of TLR4 gene and its effects on milk somatic cell score in Chinese commercial cattle. Molecular Biology Reports 41 23452351
Wiggans, GR & Shook, GE 1987 A lactation measure of somatic cell count. Journal of Dairy Science 70 26662672
Winfrey, MR, Rott, MA & Wortman, AT 1997 Unraveling DNA: Molecular Biology for the Laboratory. Upper Saddle River, NJ, USA: Prentice-Hall
Zhou, H, Cheng, L, Gong, H, Byun, SO, Edwards, GR, & Hickford, J 2017 Variation in the toll-like receptor 4 (tlr4) gene affects milk traits in dairy cows. Journal of Dairy Research 84 426429

Keywords

Type Description Title
PDF
Supplementary materials

Wang et al. supplementary material
Wang et al. supplementary material 1

 PDF (321 KB)
321 KB

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed