A SspI PCR-RFLP detecting a silent allele at the goat CSN2 locus

Gianfranco Cosenza; Alfredo Pauciullo; Daniela Gallo; Dino Di Berardino; Luigi Ramunno

doi:10.1017/S0022029905001342

Abstract

The goat calcium-sensitive caseins (αs1, β and αs2) represent, over many years, an excellent model for demonstrating that the major part of the variability observed in the content of these proteins in goat milk is mostly due to the presence of autosomal alleles at single structural loci (CSN1S1, CSN2 and CSN1S2 respectively) clustered on a 200 kb segment of chromosome 6; furthermore, CSN1S1 and CSN2 are convergently transcribed and are only 12 kb apart (Rijnkels, 2002).

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Caroli, A. Chiatti, F. Chessa, S. Rignanese, D. Bolla, P. and Pagnacco, G. 2006. Focusing on the Goat Casein Complex. Journal of Dairy Science, Vol. 89, Issue. 8, p. 3178.

Caroli, A. Chiatti, F. Chessa, S. Rignanese, D. Ibeagha-Awemu, E.M. and Erhardt, G. 2007. Characterization of the Casein Gene Complex in West African Goats and Description of a New αs1-Casein Polymorphism. Journal of Dairy Science, Vol. 90, Issue. 6, p. 2989.

Moatsou, Golfo Mollé, Daniel Moschopoulou, Ekaterini and Valérie, Gagnaire 2007. Study of Caprine β-casein using Reversed-phase High-performance Liquid Chromatography and Mass Spectroscopy: Identification of a New Genetic Variant. The Protein Journal, Vol. 26, Issue. 8, p. 562.

Cosenza, G. Pauciullo, A. Colimoro, L. Mancusi, A. Rando, A. Di Berardino, D. and Ramunno, L. 2007. An SNP in the goat CSN2 promoter region is associated with the absence of β‐casein in milk. Animal Genetics, Vol. 38, Issue. 6, p. 655.

Sztankóová, Z. Kysel’ová, J. Kott, T. and Kottová, E. 2008. Technical Note: Detection of the C Allele of β-Casein (CSN2) in Czech Dairy Goat Breeds Using LightCycler Analysis. Journal of Dairy Science, Vol. 91, Issue. 10, p. 4053.

Moatsou, G. Moschopoulou, E. Mollé, D. Gagnaire, V. Kandarakis, I. and Léonil, J. 2008. Comparative study of the protein fraction of goat milk from the Indigenous Greek breed and from international breeds. Food Chemistry, Vol. 106, Issue. 2, p. 509.

Chessa, S. Rignanese, D. Küpper, J. Pagnacco, G. Erhardt, G. and Caroli, A. 2008. Short Communication: The β-Casein (CSN2) Silent Allele C1 Is Highly Spread in Goat Breeds. Journal of Dairy Science, Vol. 91, Issue. 11, p. 4433.

Küpper, Julia Chessa, Stefania Rignanese, Daniela Caroli, Anna and Erhardt, Georg 2010. Divergence at the casein haplotypes in dairy and meat goat breeds. Journal of Dairy Research, Vol. 77, Issue. 1, p. 56.

Pauciullo, A. Giambra, I.J. Iannuzzi, L. and Erhardt, G. 2014. The β-casein in camels: molecular characterization of the CSN2 gene, promoter analysis and genetic variability. Gene, Vol. 547, Issue. 1, p. 159.

Selvaggi, Maria Laudadio, Vito Dario, Cataldo and Tufarelli, Vincenzo 2014. Major proteins in goat milk: an updated overview on genetic variability. Molecular Biology Reports, Vol. 41, Issue. 2, p. 1035.

Tortorici, Lina Di Gerlando, Rosalia Mastrangelo, Salvatore Sardina, Maria T. and Portolano, Baldassare 2014. Genetic Characterisation ofCSN2Gene inGirgentanaGoat Breed. Italian Journal of Animal Science, Vol. 13, Issue. 4, p. 3414.

Montalbano, Maria Segreto, Roberta Di Gerlando, Rosalia Mastrangelo, Salvatore and Sardina, Maria Teresa 2016. Quantitative determination of casein genetic variants in goat milk: Application in Girgentana dairy goat breed. Food Chemistry, Vol. 192, Issue. , p. 760.

Cosenza, G. Iannaccone, M. Pico, B. A. Ramunno, L. and Capparelli, R. 2016. The SNP g.1311T>C associated with the absence of β‐casein in goat milk influences CSN2 promoter activity. Animal Genetics, Vol. 47, Issue. 5, p. 615.

BOZKAYA, Faruk YİĞİN, Akın and ATLI, Mehmet Osman 2019. Kilis, Halep ve Kıl Keçilerinde Beta-Kazein (CSN2) Genindeki Çeşitliliğin Allel Spesifik PCR, Real-Time PCR ve Sekans Analizi ile Araştırılması. Harran Üniversitesi Veteriner Fakültesi Dergisi, Vol. 8, Issue. 1, p. 70.

Rahmatalla, Siham A. Arends, Danny Said Ahmed, Ammar Hassan, Lubna M. A. Krebs, Stefan Reissmann, Monika and Brockmann, Gudrun A. 2021. Capture Sequencing to Explore and Map Rare Casein Variants in Goats. Frontiers in Genetics, Vol. 12, Issue. ,

Nicolai, Maria Adalgisa Garro, Giuseppina Caira, Simonetta Mauriello, Rosalba Quarto, Maria De Pascale, Sabrina and Chianese, Lina 2021. Occurrence of quantitative genetic polymorphism at the caprine β-CN locus, as determined by a proteomic approach. International Dairy Journal, Vol. 112, Issue. , p. 104855.

Li, Xu Spencer, Garrick W.K. Ong, Lydia and Gras, Sally L. 2022. Beta casein proteins – A comparison between caprine and bovine milk. Trends in Food Science & Technology, Vol. 121, Issue. , p. 30.

Rahmatalla, Siham A. Arends, Danny and Brockmann, Gudrun A. 2022. Review: Genetic and protein variants of milk caseins in goats. Frontiers in Genetics, Vol. 13, Issue. ,

Cosenza, Gianfranco Albarella, Sara D’Anza, Emanuele Iannuzzi, Alessandra Selvaggi, Maria Pugliano, Mariagiulia Galli, Tiziana Saralli, Giorgio Ciotola, Francesca and Peretti, Vincenzo 2023. A New AS-PCR Method to Detect CSN201 Allele, Genotyping at Ca-Sensitive Caseins Loci and Milk Traits Association Studies in Autochthonous Lazio Goats. Animals, Vol. 13, Issue. 2, p. 239.

Article contents

A SspI PCR-RFLP detecting a silent allele at the goat CSN2 locus

Abstract

Keywords

Access options

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Article contents

A SspI PCR-RFLP detecting a silent allele at the goat CSN2 locus

Abstract

Keywords

Access options

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests