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Recovery of whey proteins from precipitated complexes of carboxymethyl cellulose and protein

Published online by Cambridge University Press:  01 June 2009

R. D. Hill
Affiliation:
Division of Food Research, Dairy Research Laboratory, C.S.I.R.O., Highett, Victoria, Australia
J. G. Zadow
Affiliation:
Division of Food Research, Dairy Research Laboratory, C.S.I.R.O., Highett, Victoria, Australia

Summary

The recovery of proteins from whey by complex formation with carboxymethyl cellulose (CMC) has been studied. After precipitation of the CMC–whey protein complex at pH 3·2, the complex was separated, washed and redissolved at pH 7. The CMC was separated from the protein by precipitation as its Ca salt. By this method protein concentrations in the supernatant liquor of up to 6 times that of the original whey were achieved. Between 10 and 70% of the protein was precipitated with the Ca CMC, depending on the pH and the excess of Ca present. The effective protein binding capacity of the recovered CMC decreased on re-use because of firm binding of both protein and Ca to the CMC.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1978

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References

REFERENCES

American Society for Testing and Materials (1971). ASTM D-1439–65.Google Scholar
Cluskey, F. J.Thomas, E. L. & Coulter, S. T. (1973). Journal of Dairy Science 52, 1181.CrossRefGoogle Scholar
Dunkerley, J. A., Hayes, J. F. (1967). Australian Journal of Dairy Technology 22, 19.Google Scholar
Fleck, A., Munro, H. N. (1965). Clinica Chimica Acta 11, 2.CrossRefGoogle Scholar
Hansen, P. M. T., Chang, J. C. (1968). Journal of Agricultural and Food Chemistry 16, 77.CrossRefGoogle Scholar
Hansen, P. M. T., Hidalgo, J. & Gould, I. A. (1971). Journal of Dairy Science 54, 830.CrossRefGoogle Scholar
Hill, R. D. & Zadow, J. G. (1974). Journal of Dairy Research 41, 373.CrossRefGoogle Scholar
Klug, E. D. & Tinsley, J. S. (1950). United States Patent 2517577.Google Scholar
Rowland, S. J. (1938). Journal of Dairy Research 9, 42.CrossRefGoogle Scholar
Zadow, J. G. (1975). Svensk Papperstidning 78, 376.Google Scholar