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Polyclonal antibodies to human milk caseins

Published online by Cambridge University Press:  01 June 2009

Marta Šimíčková ,
Bohumír A. Lang  and
Ladislav Slepička
Marta Šimíčková
Affiliation:
Institutes of Medical Research, Research Institute of Clinical and Experimental Oncology, 656 53 Brno, Czechoslovakia
Bohumír A. Lang
Affiliation:
Institutes of Medical Research, Research Institute of Clinical and Experimental Oncology, 656 53 Brno, Czechoslovakia
Ladislav Slepička
Affiliation:
Institute of Sera and Vaccines, 775 00 Olomouc, Czechoslovakia
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Summary

Preparations of total human casein and its individual fractions were isolated for production of specific polyclonal antibodies. Immunization procedures used differed in the schedule for antigen administration, antigen concentrations, degree of additional purification, and modification of the size of molecules. Immunoprecipitation techniques failed to provide an unambiguous proof of the presence of antibodies to human milk caseins in antisera even after immunization of animals phylogenetically considerably different from man. Immunoblotting, however, allowed antibodies to β-casein to be identified and compared with available monoclonal antibodies.

Type
Original articles
Information
Journal of Dairy Research , Volume 58 , Issue 1 , February 1991 , pp. 115 - 125
Copyright
Copyright © Proprietors of Journal of Dairy Research 1991

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