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Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation

  • Giuseppe Piccione (a1), Vanessa Messina (a1), Angelo Schembari (a1), Stefania Casella (a1), Claudia Giannetto (a1) and Daniela Alberghina (a1)...

Abstract

In dairy cows the period of transition from late gestation to early lactation is recognized as inducing considerable metabolic adaptation. The aim of this study was to analyse modifications in serum protein values occurring during the dry and the transition period and during lactation in a group of five Holstein cows of high average milk production. For all subjects, selected on the basis of their pregnancy status, blood samples were collected at different physiological phases: dry period (−60, −30 d to calving), transition period (almost 7 d to calving, 7 d after calving), and lactation (weeks 2, 5 and 15 after calving), for a total of eight blood samples for each cow. On each blood sample total proteins and electrophoresis analysis were performed. On the data obtained, normally distributed (P<0·05, Kolmogorov-Smirnov's test), one-way Repeated Measure Analysis of Variance (ANOVA), was applied to evaluate the influence of different stages of gestation and lactation on the considered parameters. Results showed a significant effect on total proteins, α1-globulins, β-globulins, γ-globulins and albumin/globulin ratio. Most of the detected modifications were related to the transition from gestation to lactation, indicating that it is a period of great metabolic stress for cows. On the basis of the obtained results we can affirm that the pattern of serum protein fraction rn could give information about dehydration, plasma volume expansion and hepatic function occurring during the peripartum period in dairy cows.

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Corresponding author

*For correspondence; e-mail: giuseppe.piccione@unime.it

References

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Alberghina, D, Casella, S, Vazzana, I, Ferrantelli, V, Giannetto, C & Piccione, G 2010 Analysis of serum proteins in clinically healthy goats (Capra hircus) using agarose gel electrophoresis. Veterinary Clinical Pathology 39 317321
Alberghina, D, Giannetto, C, Vazzana, I, Ferrantelli, V & Piccione, G 2011 Reference intervals for total protein concentration, serum protein fractions, and albumin/globulin ratios in clinically healthy dairy cow. Journal of Veterinary Diagnostic Investigations 23 111114
Bonfatti, V, Di Martino, G, Cecchinato, A, Vicario, D & Carnier, P 2010 Effects of beta-kappa-casein (CSN2-CSN3) haplotypes and beta-lactoglobulin (BLG) genotypes on milk production traits and detailed protein composition of individual milk of Simmental cows. Journal of Dairy Science 93 37973808
Bush, BM 1991 Nutrients and metabolites. In: Interpretation of Laboratory Results for Small Animal Clinicians, pp. 221310. Osney Mead, Oxford, UK: Blackwell Scientific Publications
Cairoli, F, Battocchio, M, Veronesi, MC, Brambilla, D, Conserva, F, Eberini, I, Wait, R & Gianazza, E 2006 Serum protein pattern during cow pregnancy: Acute-phase proteins increase in the peripartum period. Electrophoresis 27 16171625
Castillo, C, Hernandez, J, Bravo, A, Lopez-Alonso, M, Pereira, V & Bendito, JL 2005 Oxidative status during late pregnancy and early lactation in dairy cows. Veterinary Journal 169 286292
Grünberg, W, Donkin, SS & Constable, PD 2011 Periparturient effects of feeding a low dietary cation-anion difference diet on acid-base, calcium, and phosphorus homeostasis and on intravenous glucose tolerance test in high producing dairy cows. Journal of Dairy Science 94 727745
Hopster, H, Bruckmaier, RM, van der Werf, JTN, Korte, SM, Machuova, J, Korte-Bouws, G & van Reenen, CG 2002 Stress responses during milking; comparing conventional and automatic milking in primiparous dairy cows. Journal of Dairy Science 85 32063216
Jansakul, AC, King, RG, Boura, ALA, Brennecke, SP & Handberg, GM 1989 Plasma levels of atrial natriuretic peptides during pregnancy and post partum in the rat. Journal of Endocrinology 120 113117
Larson, BL & Kendall, KA 1957 Changes in specific blood serum protein levels associated with parturition in the bovine. Journal of Dairy Science 40 659666
Little, W 1974 An effect of the stage of lactation on the concentration of albumin in the serum of dairy cows. Research in Veterinary Science 17 193199
Kaneko, JJ 1997 Serum proteins and dysproteinemias. In: Clinical Biochemistry of Domestic Animals (Eds Kaneko, JJ, Harvey, JH & Bruss, ML) pp. 117138. London, UK: Academic Press
Mallard, BA, Dekkers, JC, Ireland, MJ, Leslie, KE, Sharif, S, Lacey-Vankampen, C, Wagter, L & Wilkie, BN 1998 Alteration in immune responsiveness during the peripartum period and its ramification on dairy cow and calf health. Journal of Dairy Science 81 585595
McLennan, MW & Willoughby, RA 1973 Observations on ionized calcium in the plasma of cows during estrus. Canadian Veterinary Journal 14 229232
Ontsouka, CE, Bruckmaier, RM & Blum, JW 2003 Fractionized milk composition during removal of colostrum and mature milk. Journal of Dairy Science 86 20052011
Park, AF, Shirley, JE, Titgemeyer, EC, Cochran, RC, DeFrain, JM, Wickersham, EE & Johnson, DE 2010 Characterization of plasma metabolites in Holstein dairy cows during the periparturient period. International Journal of Dairy Science 5 253263
Polakova, K, Kudrna, V, Kodes, A, Hucko, B & Mudrik, Z 2010. Non-structural carbohydrates in the nutrition of high yielding dairy cows during a transition period. Czech Journal of Animal Science 55 468478
Rowlands, GJ & Manston, R 1983 Decline of serum albumin concentration at calving in dairy cow: its relationship with age and association with subsequent fertility. Research in Veterinary Science 34 9096
Rowlands, GJ, Manston, R, Stark, AJ, Russel, AM, Collis, KA & Collis, SC 1980 Changes in albumin, globulin, glucose and cholesterol concentrations in the blood of dairy cows in late pregnancy and early lactation and relationships with subsequent fertility. Journal of Agricultural Science 94 517527
Sánchez, L, Aranda, P, Pérez, MD & Calvo, M 1983 Concentration of lactoferrin and transferrin throughout lactation in cow's colostrum and milk. Biological Chemistry Hoppe Seyler 369 10051008
Schopen, GC, Heck, JM, Bovenhuis, H, Visker, MH, van Valenberg, HJ & van Arendonk, JA 2009 Genetic parameters for major milk proteins in Dutch Holstein-Friesians. Journal of Dairy Science 92 11821191
Stelwagen, K, Carpenter, E, Haigh, B, Hodgkinson, A & Wheeler, TT 2009 Immune components of bovine colostrum and milk. Journal of Animal Science 87 39
Van Dorland, HA, Richter, S, Morel, I, Doherr, MG, Castro, N & Bruckmaier, RM 2009 Variation in hepatic regulation of metabolism during the dry period and in early lactation in dairy cows. Journal of Dairy Science 92 19241940

Keywords

Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation

  • Giuseppe Piccione (a1), Vanessa Messina (a1), Angelo Schembari (a1), Stefania Casella (a1), Claudia Giannetto (a1) and Daniela Alberghina (a1)...

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