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Optimisation of medium composition for probiotic biomass production using response surface methodology

Published online by Cambridge University Press:  19 December 2013

Masumeh Anvari ,
Gholam Khayati  and
Shora Rostami
Masumeh Anvari*
Affiliation:
Department of Microbiology, Faculty of Sciences, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht, Iran
Gholam Khayati
Affiliation:
Department of Chemical Engineering, Faculty of Eng., Guilan University, P. O. Box 3756-41635, Rasht, Iran
Shora Rostami
Affiliation:
Department of Microbiology, Sciences and Research Branch, Islamic Azad University, Guilan, Iran
*
*Corresponding author; e-mail: anvari@iaurasht.ac.ir
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Abstract

This study was aimed to optimise lactose, inulin and yeast extract concentration and also culture pH for maximising the growth of a probiotic bacterium, Bifidobacterium animalis subsp. lactis in apple juice and to assess the effects of these factors by using response surface methodology. A second-order central composite design was applied to evaluate the effects of these independent variables on growth of the microorganism. A polynomial regression model with cubic and quadratic terms was used for analysis of the experimental data. It was found that the effects involving inulin, yeast extract and pH on growth of the bacterium were significant, and the strongest effect was given by the yeast extract concentration. Estimated optimum conditions of the factors on the bacterial growth are as follows: lactose concentration=9·5 g/l; inulin concentration=38·5 mg/l; yeast extract concentration=9·6 g/l and initial pH=6·2.

Keywords

Bifidobacterium animalis subsp. Lactisprobioticresponse surface methodologyapple juice
Type
Research Article
Information
Journal of Dairy Research , Volume 81 , Issue 1 , February 2014 , pp. 59 - 64
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013 

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References

Akalın, AS, Fenderya, S & Akbulut, N 2004 Viability and activity of Bifidobacteria in yoghurt containing fructo oligosaccharide during refrigerated storage. International Journal of Food Science and Technology 39 613–621 Google Scholar
Alander, M & Mattila-Sandholm, T 2000 Functional foods for EU-Health in 2000, in 4th Workshop, FAIR CT96-1028, PROBDEMO, VTT Symposium 198, Rovaniemi, Finland Google Scholar
Avonts, L, Van Uytven, E & De Vuyst, L 2004 Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media. International Dairy Journal 14 947–955 Google Scholar
Bousvaros, A, Guandalini, S, Baldassano, RN, Botelho, C, Evans, J, Ferry, GD, Goldin, B, Hartigan, L, Kugathasan, S, Levy, J, Murray, KF, Oliva-Hemker, M, Rosh, JR, Tolia, V, Zholudev, A, Vanderhoof, JA & Hibberd, PL 2005 A randomized, double-blind trial of Lactobacillus GG versus placebo in addition to standard maintenance therapy for children with Crohn's disease. Inflammatory Bowel Disease 11 833–839 Google Scholar
Bruno, A, Lankaputhra, WEV & Shah, NP 2002 Growth viability and activity of Bifidobacterium spp. in skim milk containing prebiotics. Journal of Food Science 67 2740–2744 Google Scholar
Costa, MGM, Fonteles, TV, de Jesus, ALT & Rodrigues, S 2013 Sonicated pineapple juice as substrate for L. casei cultivation for probiotic beverage development: Process optimisation and product stability. Food Chemistry 139 261–266 CrossRefGoogle ScholarPubMed
Ding, WK & Shah, NP 2008 Survival of free and microencapsulated probiotic bacteria in orange and apple juices. International Food Research Journal 15 219–232 Google Scholar
Doleyres, Y & Lacroix, C 2005 Technologieswith free and immobilised cells for probiotic bifidobacteria production and protection. International Dairy Journal 15 973–988 Google Scholar
Doleyres, Y, Fliss, I & Lacroix, C 2002 Quantitative determination of the spatial distribution of pure- and mixed-strain immobilized cells in gel beads by immunofluorescence. Applied Microbiology and Biotechnology 59 297–302 Google ScholarPubMed
Du Toit, M, Engelbrecht, L, Lerm, E & Krieger-Weber, S 2011 Lactobacillus: The next generation of malolactic fermentation starter cultures—An overview. Food Bioprocess and Technology 4 876–906 Google Scholar
Elbert, EM & Esselen, WB 1959 Free Amino Acids and Thiamine of Apple Juice and their relation of Fermentation. Journal of Food Science 24 352–361 CrossRefGoogle Scholar
Fonteles, TV, Costa, MG, de Jesus, ALT & Rodrigues, S 2012 Optimization of the fermentation of cantaloupe juice by Lactobacillus casei NRRL B-442. Food and Bioprocess Technology 5 2819–2826 CrossRefGoogle Scholar
Guarner, F & Schaafsma, GJ 1998 Probiotics. International Journal of Food Microbiology 39 237–238 Google Scholar
Heller, KJ 2001 Probiotic bacteria in fermented foods: product characteristics and starter organisms. American Journal of Clinical Nutrition 73 374–379 Google Scholar
Khayati, G 2013 Optimization of Propionic Acid Extraction by Aqueous Two-Phase System Using Response Surface Methodology. Chemical Engineering Communications 200 667–677 Google Scholar
Khayati, G & Kiyani, F 2012 A statistical approach for optimization of lipase production by using rice straw: analysis of different inducers and nitrogen sources effect. Minerva Biotecnologica 24 83–89 Google Scholar
Kodaka, H, Mizuochi, S, Teramura, H & Nirazuka, T 2005 Comparison of the compact dry TC method with the standard pour plate method (AOAC Official Method 966·23) for determining aerobic colony counts in food samples: performance-tested method SM. Journal of AOAC International 88 1702–1713 Google Scholar
Kuehbacher, T, Ott, SJ, Helwig, U, Mimura, T, Rizzello, F, Kleessen, B, Gionchetti, P, Blaut, M, Campieri, M, Folsch, UR, Kamm, MA & Schreiber, S 2006 Bacterial and fungal microbiota in relation to probiotic therapy (VSL#3) in pouchitis. Gut 55 833–841 Google Scholar
Lee, WC, Yusof, S, Hamid, NSA & Baharin, BS 2006 Optimizing conditions for hot water extraction of banana juice using response surface methodology (RSM). Journal of Food Engineering 75 473–479 CrossRefGoogle Scholar
Lin, DC 2003 Probiotics as functional foods. Nutrition and Clinical Practice 18 497–506 CrossRefGoogle ScholarPubMed
Liyana-Pathirana, C & Shahidi, F 2005 Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chemistry 93 47–56 CrossRefGoogle Scholar
Lourens-Hattingh, A & Viljoen, BC 2001 Yogurt as probiotic carrier food. International Dairy Journal 11 1–17 CrossRefGoogle Scholar
Montgomery DC 2001 Design and Analysis of Experiments. 5th edition. New York: John Wiley and Sons Google Scholar
Mousavi, ZE, Mousavi, SM, Razavi, SH, Emam-Djomeh, Z & Kiani, H 2011 Fermentation of pomegranate juice by probiotic lactic acid bacteria. World Journal of Microbiology and Biotechnology 27 123–128 Google Scholar
Nath, A & Chattopadhyay, PK 2007 Optimization of oven toasting for improving crispness and other quality attributes of ready to eat potato–soy snack using response surface methodology. Journal of Food Engineering 80 1282–1292 Google Scholar
Oliveira, MN & Damin, MR 2003 Effect of total solids and sucrose contents on acidity, firmness and viability of yogurt and probiotic bacteria in fermented milk. Ciência e Tecnologia de Alimentos 23 172–176 Google Scholar
Pereira, ALF, Maciel, TC & Rodrigues, S 2011 Probiotic beverage from cashew apple juice fermented with Lactobacillus casei . Food Research International 44 1276–1283 Google Scholar
Petschow, BW & Talbott, RD 1990 Growth promotion of Bifidobacterium species by whey and casein fractions from human and bovine milk. Journal of Clinical Microbiology 28 287–292 Google Scholar
Poch, M & Bezkorovainy, A 1988 Growth-enhancing supplements for various pieces of the genus Bifidobacterium . Journal of Dairy Science 71 3214–3221 CrossRefGoogle Scholar
Poolman, B & Konings, WN 1988 Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport. Journal of Bacteriology 170 700–707 Google Scholar
Reid, G, Charbonneau, D, Erb, J, Kochanowski, B, Beuerman, D, Poehner, R & Bruce, AW 2003 Oral use of Lactobacillus rhamnosus GR-1 and L. fermentum RC-14 significantly alters vaginal flora: randomized, placebo-controlled trial in 64 healthy women. FEMS Immunology and Medicinal Microbiology 35 131–134 CrossRefGoogle ScholarPubMed
Rozada-Sánchez, R, Sattur, AP, Thomas, K & Pandiella, SS 2008 Evaluation of Bifidobacterium spp. for the production of a potentially probiotic malt-based beverage. Process Biochemistry 43 848–854 CrossRefGoogle Scholar
Shin, HS, Lee, JH, Pestka, JJ & Ustunol, Z 2000 Growth and viability of commercial Bifidobacterium spp. in skim milk containing oligosaccharides and inulin. Journal of Food Science 65 885–887 Google Scholar
Sin, HN, Yusof, S, Hamid, NSA & Rahman, RA 2006 Optimization of enzymatic clarification of sapodilla juice using response surface methodology. Journal of Food Engineering 73 313–319 Google Scholar
Szymanski, H, Pejcz, J, Jawien, M, Chmielarczyk, A, Strus, M & Heczko, PB 2006 Treatment of acute infectious diarrhoea in infants and children with a mixture of three Lactobacillus rhamnosus strains—a randomized, doubleblind, placebo-controlled trial. Alimentary Pharmacology & Therapeutics 23 247–253 Google Scholar
Weinbreck, F, Bodnár, I & Marco, ML 2010 Can encapsulation lengthen the shelf-life of probiotic bacteria in dry products? International Journal of Food Microbiology 136 364–367 Google Scholar