Hostname: page-component-84b7d79bbc-c654p Total loading time: 0 Render date: 2024-07-26T08:01:06.579Z Has data issue: false hasContentIssue false
  • English
  • Français

Oestradiol enhances apoptosis in bovine mammary epithelial cells in vitro

Published online by Cambridge University Press:  14 December 2012

Lucile Yart ,
Laurence Finot ,
Vanessa Lollivier  and
Frederic Dessauge
Lucile Yart
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
Laurence Finot
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
Vanessa Lollivier
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France Université européenne de Bretagne, France
Frederic Dessauge*
Affiliation:
INRA, UMR1348 Pegase, F-35590 Saint-Gilles, France Agrocampus Ouest, UMR1348 Pegase, F-35590 Rennes, France
*
*For correspondence; e-mail: frederic.dessauge@rennes.inra.fr
Get access Rights & Permissions [Opens in a new window]

Abstract

Ovarian steroids, oestradiol and progesterone, are required for normal mammary growth at puberty and during pregnancy. They contribute to mammary parenchyma development by stimulating mammary epithelial cell (MEC) proliferation. However several studies demonstrate that oestradiol negatively affects milk production during the declining phase of lactation, but the oestradiol effect on MEC in lactating mammary gland remains unclear. The objective of this study was to investigate the differential effect of oestradiol on bovine MECs mimicking two physiological statuses: active and early apoptotic MECs. We demonstrated that oestradiol has a major effect on early apoptotic MECs and might accelerate MEC apoptosis by activation of caspases rather than by inducing apoptosis in active MECs. Early apoptotic MECs could be compared with senescent cells in the late-lactation mammary gland. These results suggest that the negative effect of oestradiol on milk production during the declining phase of lactation would be due to an enhancement of apoptotic processes in MECs.

Keywords

Oestradiolmammary epithelial cellapoptosiscowMAC-T cell
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 1 , February 2013 , pp. 113 - 121
Copyright
Copyright © Proprietors of Journal of Dairy Research 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aker, RM 2002 Lactation and the Mammary Gland. Ames: Blackwell PublishingGoogle Scholar
Athie, F, Bachman, KC, Head, HH, Hayen, MJ & Wilcox, CJ 1996 Estrogen administered at final milk removal accelerates involution of bovine mammary gland. Journal of Dairy Science 79 220–226CrossRefGoogle ScholarPubMed
Berry, SD, Jobst, PM, Ellis, SE, Howard, RD, Capuco, AV & Akers, RM 2003a Mammary epithelial proliferation and estrogen receptor alpha expression in prepubertal heifers: effects of ovariectomy and growth hormone. Journal of Dairy Science 86 2098–2105CrossRefGoogle ScholarPubMed
Berry, SD, Weber Nielsen, MS, Sejrsen, K, Pearson, RE, Boyle, PL & Akers, RM 2003b Use of an immortalized bovine mammary epithelial cell line (MAC-T) to measure the mitogenic activity of extracts from heifer mammary tissue: effects of nutrition and ovariectomy. Domestic Animal Endocrinology 25 245–253Google Scholar
Capuco, AV, Wood, D, Baldwin, R, Mcleod, K & Paape, M 2001 Mammary cell number, proliferation, and apoptosis during a bovine lactation: relation to milk production and effect of bST. Journal of Dairy Science 84 2177–2187CrossRefGoogle ScholarPubMed
Clarke, R 2000 Introduction and overview: sex steroids in the mammary gland. Journal of Mammary Gland Biology and neoplasia 5 245–50CrossRefGoogle ScholarPubMed
Delbecchi, L & Lacasse, P 2006 Suppression of estrous cycles in lactating cows has n effect on milk production. Journal of Dairy Science 89 636–639Google Scholar
Delbecchi, L, Miller, M, Prud'homme, C, Petitclerc, D, Wagner, GF & Lacasse, P 2005 17beta-oestradiol reduces milk synthesis and increases stanniocalcin gene expression in the mammary gland of lactating cows. Livestock Production Science 98 57–66Google Scholar
Desjardins, C, Paape, MJ & Tucker, HA 1968 Contribution of pregnancy, fetuses, fetal placentas and deciduomas to mammary gland and uterine development. Endocrinology 83 907–910Google Scholar
Forsyth, IA 1986 Variation among species in the endocrine control of mammary growth and function: the roles of prolactin, growth hormone, and placental lactogen. Journal of Dairy Science 69 886–903Google Scholar
Hurley, WL 1989 Mammary gland function during involution. Journal of Dairy Science 72 1637–1646Google Scholar
Huynh, HT, Robitaille, G & Turner, JD 1991 Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation. Experimental Cell Research 197 191–199Google Scholar
Jordan, VC, Lewis, JS, Osipo, C & Cheng, D 2005 The apoptotic action of estrogen following exhaustive antihormonal therapy: a new clinical treatment strategy. Breast 14 624–630Google Scholar
Kolek, O, Gajkowska, B, Godlewski, MM & Motyl, T 2003 Antiproliferative and apoptotic effect of TGF-beta 1 in bovine mammary epithelial BME-UV1 cells. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology 134 417–430Google ScholarPubMed
Lewis, JS, Meeke, K, Osipo, C, Ross, EA, Kidawi, N, Li, T, Bell, E, Chandel, NS & Jordan, VC 2005 Intrinsic mechanism of estradiol-induced apoptosis in breast cancer cells resistant to estrogen deprivation. Journal of the National Cancer Institute 97 1746–1759Google Scholar
Mollett, TA, Erb, RE, Monk, EL & Malven, PV 1976 Changes in estrogen, progesterone, prolactin and lactation traits associated with injection of estradiol-17beta and progesterone into lactating cows. Journal of Animal Science 42 655–663CrossRefGoogle ScholarPubMed
Mor, G, Kohen, F, Garcia-Velasco, J, Nilsen, J, Brown, W, Song, J & Naftolin, F 2000 Regulation of fas ligand expression in breast cancer cells by estrogen: functional differences between estradiol and tamoxifen. Journal of Steroid Biochemistry and Molecular Biology. 73 185–194Google Scholar
Patel, OV, Takenouchi, N, Takahashi, T, Hirako, M, Sasaki, N & Domeki, I 1999 Plasma oestrone and oestradiol concentrations throughout gestation in cattle: relationship to stage of gestation and fetal number. Research in Veterinary Science 66 129–33Google Scholar
Russo, J, Yang, X, Hu, YF, Bove, BA, Huang, Y, Silva, ID, Tahin, Q, Wu, Y, Higgy, N, Zekri, A & Russo, IH 1998 Biological and molecular basis of human breast cancer. Frontiers in Bioscience 3 D944–D960CrossRefGoogle ScholarPubMed
Stefanon, B, Colitti, M, Gabai, G, Knight, CH & Wilde, CJ 2002 Mammary apoptosis and lactation persistency in dairy animals. Journal of Dairy Research 69 37–52Google Scholar
Tucker, HA 2000 Hormones, mammary growth, and lactation: a 41-year perspective. Journal of Dairy Science 83 874–884Google Scholar
Wilde, CJ & Knight, CH 1989 Metabolic adaptations in mammary gland during the declining phase of lactation. Journal of Dairy Science 72 1679–1692Google Scholar
Woodward, TL, Beal, WE & Akers, RM 1993 Cell interactions in initiation of mammary epithelial proliferation by oestradiol and progesterone in prepubertal heifers. Journal of Endocrinology 136 149–157Google Scholar
Yart, L, Finot, L, Marnet, PG & Dessauge, F 2012a Suppression of ovarian secretions before puberty strongly affects mammogenesis in the goat. Journal of Dairy Research 79 157–167Google Scholar
Yart, L, Dessauge, F, Finot, L, Barbey, S, Marnet, PG & Lollivier, V 2012b Ovariectomy improves lactation persistency in dairy cows. Journal of Dairy Science 95 3794–3802Google Scholar
Zarzynska, J, Gajewska, M & Motyl, T 2005 Effects of hormones and growth factors on TGF-beta1 expression in bovine mammary epithelial cells. Journal of Dairy Research 72 39–48Google Scholar