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Interferences on microbial inhibitor tests related to ivermectin treatment in lactating dairy goats

Published online by Cambridge University Press:  07 September 2016

Tamara Romero ,
Vicente Javier Moya ,
Nemesio Fernández ,
Rafael Althaus ,
Wim Reybroeck  and
María Pilar Molina
Tamara Romero*
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Vicente Javier Moya
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Nemesio Fernández
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
Rafael Althaus
Affiliation:
Cátedra de Biofísica, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral. R.P.L., Kreder. 3080. Esperanza, Argentina
Wim Reybroeck
Affiliation:
Institute for Agricultural and Fisheries Research, Technology and Food Science Unit (ILVO-T&V), Brusselsesteenweg 370, 9090 Melle, Belgium
María Pilar Molina
Affiliation:
Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, Camino de Vera, s/n. 46022. Valencia, Spain
*
*For correspondence; e-mail: tarorue@upvnet.upv.es
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Abstract

This Research Communication reports interferences related to the administration of ivermectin in lactating dairy goats on the response of microbial tests for screening antibiotics in milk. Twenty-eight Murciano-Granadina goats, naturally infested with Sarcoptes scabiei var. caprae, were treated with a subcutaneous injection of ivermectin (200 µg/kg b.w.). To prevent re-infestation, a second dose was applied 7 d later. Individual milk samples were collected, daily, up to 15 d post-treatment. Milk samples were analysed by microbial inhibitor tests (BRT MRL, Delvotest SP-NT MCS and Eclipse 100) and ivermectin residues were quantified by HPLC. A large number of positive results were obtained for all microbial tests, especially on the first day after treatment (BRT MRL = 46·4%; Delvotest SP-NT MCS = 14·3%; and Eclipse 100 = 17·8%). However, the highest concentration of drug residues in milk (24·3 ng/ml) was detected on the tenth day after treatment, when positive outcomes were relatively lower (BRT MRL = 17·8%; Delvotest SP-NT MCS = 10·7%; and Eclipse 100 = 7·4%). Results herein suggest that factors related to the ivermectin treatment other than drug residues in milk, or alterations produced by the parasitic disease itself affecting the immune response of animals, could be the cause of false-positive results in microbial tests. It can be concluded that the application of ivermectin in dairy goats infested with sarcoptes mange during lactation produces persistent drug residues in milk, and could also cause false-positive results in microbial inhibitor tests for screening antibiotics.

Keywords

Goat milkivermectinmicrobial inhibitor tests
Type
Research Article
Information
Journal of Dairy Research , Volume 83 , Issue 3 , August 2016 , pp. 341 - 344
Copyright
Copyright © Proprietors of Journal of Dairy Research 2016 

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