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Inorganic constituents of cheese: analysis of juice from a one-month-old Cheddar cheese and the use of light and electron microscopy to characterize the crystalline phases

  • Howard A. Morris (a1), Carl Holt (a1), Brian E. Brooker (a2), Jean M. Banks (a1) and William Manson (a1)...


A method of extracting cheese juice is described. Juice from a single one-month-old Cheddar cheese was used to investigate the relationship between the composition of the cheese juice and the occurrence of crystalline aggregates in the cheese. Two classes of crystalline aggregates were identified by light microscopy and also by electron microscopy in combination with X-ray microanalysis: the larger class contained both Ca and P whereas the smaller class contained Ca but not P. Concentrations of ions in the cheese and cheese juice were determined and corrected for the excluded volume of co-solutes and other phases present. When this was done, comparison of the concentrations of Na, K, Cl and lactate in the cheese and the cheese juice indicated that they were virtually entirely in solution, whereas appreciable amounts of the total Ca, P and P1 in the cheese were not extracted in the cheese juice. The amounts of the non-extractable salts were consistent with the volume fractions of the crystalline aggregates found by microscopy; calculations of the ion equilibria in the cheese juice showed that it was supersaturated with respect to various Ca phosphate salts and to tricalcium citrate. It is concluded that the physicochemical origin of the crystalline aggregates was the nucleation and growth of crystals from a supersaturated solution, though the sites of nucleation may have been formed by the microbial activity in the cheese. Of the free amino acids found in the juice, none was at a sufficiently high concentration to form a saturated solution, indicating that in this relatively young cheese the crystalline aggregates were not formed from amino acids.



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Allen, R. J. L. 1940 The estimation of phosphorus. Biochemical Journal 34 858865
Aston, J. W. & Creamer, L. K. 1986 Contribution of the components of the water-soluble fraction to the flavour of Cheddar cheese. New Zealand Journal of Dairy Science and Technology 21 229248
Banks, J. M. & Muir, D. D. 1985 Effect of incorporation of denatured whey protein on the yield and quality of Cheddar cheese. Journal of the Society of Dairy Technology 38 2732
Banks, J. M., Muir, D. D. & Tamime, A. Y. 1984 A comparison of cheese yield and cheesemaking efficiency using seasonal and standardized milk. Journal of the Society of Dairy Technology 37 8388
Barthel, Chr., Sandberg, E. & Haglund, E. 1928 [Research on rennin in cheeses.] Lait 8 762768
British Standards Institution 1976 Methods for the chemical analysis of cheese. London: BSI (BS No. 770).
Brooker, B. E., Hobbs, D. G. & Turvey, A. 1975 Observations on the microscopic crystalline inclusions in Cheddar cheese. Journal of Dairy Research 42 341348
Carleton, H. M. & Drury, R. A. B. 1957 Histological Technique, 3rd edn.London: Oxford University Press.
Conochie, J. & Sutherland, B. J. 1965 The nature and cause of seaminess in Cheddar cheese. Journal of Dairy Research 32 3544
Glagoleff, A. A. 1933 [On the geometrical methods of quantitative mineralogic analysis of rocks]. Trud Instituta Prikladnoiˇ Mineralogiiˇ i Metallurgiiˇ 59 1–47
Harper, W. J., Swanson, A. M. & Sommer, H. H. 1953 Observations on the chemical composition of white particles in several lots of Cheddar cheese. Journal of Dairy Science 36 368372
Holt, C. 1982 Inorganic constituents of milk. III. The colloidal calcium phosphate of cow's milk. Journal of Dairy Research 49 2938
Holt, C., Dalgleish, D. G. & Jenness, R. 1981 Calculation of the ion equilibria in milk diffusate and comparison with experiment. Analytical Biochemistry 113 154163
Johnson, M. E., Severn, D. & Olsen, N. F. 1986 Development of manufacturing and packaging processes of cheese to minimize milk salt crystallization in cheese during storage and distribution. Report of Research Activities, Walter U. Price Cheese Research Institute, University of Wisconsin 1985 pp. 2122
Kalab, M. 1980 Decayed lactic bacteria - a possible source of crystallization nuclei in cheese. Journal of Dairy Science 63 301304
Knoop, A.-M. & Peters, K.-H. 1971 [Submicroscopic structure variations during the ripening of Camembert cheese.] Milchwissenschaft 26 193198
McDowall, F. H. & Dolby, R. M. 1936 Studies on the chemistry of Cheddar cheese making. IV. Lactose and lactic acid in whey and curd; the presence of bound water in curd; the existence of a Donnan equilibrium between curd and whey; and the rate of penetration of salt into curd. Journal of Dairy Research 7 156175
Monir, A. M. M. F. 1962 The calcium-paracaseinate-phosphate-complex under conditions similar to those in cheese. Mededelingen van de Landbouwhogeschool te Wageningen, Nederland 62(10) 176
Pearce, K. N., Creamer, L. K. & Gilles, J. 1973 Calcium lactate deposits on rindless Cheddar cheese. New Zealand Journal of Dairy Science and Technology 8 37.
Sandberg, E., Haglund, E. & Barthel, Chr. 1930 [Analysis of cheese juice as a method of determining the degree of maturation.] Lait 10 121
Smith, R. M. & Martell, A. E. 1977 Critical Stability Constants, Vol. 3. New York: Plenum Press
Spackman, D. H., Stein, W. H. & Moore, S. 1958 Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30 11901206
Walstra, P. & Jenness, R. 1984 Dairy Chemistry and Physics, p. 408. New York: John Wiley and Sons
Washam, C. J., Kerr, T. J. & Hurst, V. J. 1982 Microstructure of various chemical compounds crystallized on Cheddar cheese. Journal of Food Protection 45 594596
Watt, B. K. & Merrill, A. L. 1976 Cheddar cheese-item no. 01–009. In Composition of Foods (rev.). Washington, DC: United States Department of Agriculture (Agricultural Handbook no. 8–1)
White, J. C. D. & Davies, D. T. 1958 The relation between the chemical composition of milk and the stability of the caseinate complex. I. General introduction, description of samples, methods and chemical composition of samples. Journal of Dairy Research 25 236255

Inorganic constituents of cheese: analysis of juice from a one-month-old Cheddar cheese and the use of light and electron microscopy to characterize the crystalline phases

  • Howard A. Morris (a1), Carl Holt (a1), Brian E. Brooker (a2), Jean M. Banks (a1) and William Manson (a1)...


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