Skip to main content Accessibility help
×
Home

Identification of Bacillus strains isolated from milk and cream with classical and nucleic acid hybridization methods

  • Ralf Tatzel (a1), Wolfgang Ludwig (a2), Karl Heinz Schleifer (a2) and Peter R. Wallnöfer (a1)

Summary

A total of 529 bacterial strains have been isolated from milk and cream sampled at different sites in a dairy production plant under conditions selective for aerobic sporeforming bacteria. Identification with classical methods based on morphological, physiological and biochemical criteria showed Bacillus licheniformis to be the most frequently occurring Bacillus sp. The investigation also revealed 62 unidentified strains. Classical identification methods were time consuming (3–7d), lacked specificity and —because of their dependence on phenotypic gene expression—sometimes produced ambiguous results. Consequently, a colony hybridization method developed for the identification of B. licheniformis strains and using non-radioactive labelled 23S rRNA targeted oligonucleotide probes was also used. Identification of B. licheniformis with borth classical and hybridization methods revealed diverging identification results for 70 strains.

Copyright

References

Hide All
Baird-Parker, A. C. 1963 A classification of micrococci and staphylococci based on physiological and biochemical tests. Journal of General Microbiology 30 409427
Carlisle, G. E. & Falkinham, J. O. 1989 Enzyme activities and antibiotic susceptibility of colonial variants of Bacillus subtilis and Bacillus licheniformis. Applied and Environmental Microbiology 55 30263028
Cromie, S. J., Dommett, T. W. & Schmidt, D. 1989 Changes in the microflora of milk with different pasteurization and storage conditions and aseptic packaging. Australian Journal of Dairy Technology 44 7477
Deák, T. & Timár, É. 1988 Simplified identification of aerobic spore-formers in the investigation of foods. International Journal of Food Microbiology 6 115125
Gayer-Herkert, G. 1992 [Molecular methods for the assay of microorganisms in mixed populations—a review of the most recent literature.] BioEngineering 8 5564
Gordon, R. E., Haynes, W. C. & Pang, C. H.-N. 1973 The genus Bacillus. Washington, DC: US Department of Agriculture (Agriculture Handbook no. 427)
Griffiths, M. W. & Phillips, J. D. 1990 Strategies to control the outgrowth of spores of psychrotrophic Bacillus spp. in dairy products. I. Use of naturally-occurring materials. Milchwissenschaft 45 621626
Grunstein, M. & Hogness, D. S. 1975 Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, USA 72 39613965
Kramer, J. M. & Gilbert, R. J. 1989 Bacillus cereus and other Bacillus species. In Foodborne Bacterial Pathogens, pp. 2170 (Ed. Doyle, M. P.). New York: Marcel Dekker (Food Science and Technology 31)
Meer, R. R., Baker, J., Bodyfelt, F. W. & Griffiths, M. W. 1991 Psychrotrophic Bacillus spp. in fluid milk products: a review. Journal of Food Protection 54 969979
Norris, J. R., Berkeley, R. C. W., Logan, N. A. & O'Donnell, A. G. 1981 The Genera Bacillus and Sporolactobacillus. In The Prokaryotes, pp. 17111742 (Eds Starr, M. P., Stolp, H., Trüper, H. G., Balows, A. and Schlegel, H. G.). New York: Springer Verlag
Rajarathinam, R., Shankar, P. A. & Laxminarayana, H. 1986 Incidence of aerobic spore-forming bacteria in milk. Indian Journal of Dairy Science 39 335336
Schleifer, K. H. 1990 DNA probes in food microbiology. Food Biotechnology 4 585598
Sneath, P. H. A. 1986 Endospore-forming Gram-positive rods and cocci. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 11041207 (Eds Sneath, P. H. A., Mair, N. S., Sharpe, M. E. and Holt, J. G.). Baltimore, MD: Williams and Wilkins
Tatzel, R., Ludwig, W., Schleifer, K. H. & Wallnöfer, P. R. 1994 Identification of Bacillus licheniformis by colony hybridization with 23S rRNA-targeted oligonucleotide probes. Systematic and Applied Bacteriology 17 99103
Waes, G. 1976 Aerobic mesophilic spores in raw milk. Milchwissenschaft 31 521525

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed