Hostname: page-component-7479d7b7d-68ccn Total loading time: 0 Render date: 2024-07-12T15:04:47.228Z Has data issue: false hasContentIssue false
  • English
  • Français

Hypoglycaemic effect of galactooligosaccharides in alloxan-induced diabetic rats

Published online by Cambridge University Press:  10 November 2014

Vikas Sangwan ,
Sudhir K Tomar ,
Babar Ali ,
Ram R B Singh  and
Ashish K Singh
Vikas Sangwan
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Sudhir K Tomar*
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Babar Ali
Affiliation:
Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India
Ram R B Singh
Affiliation:
Dairy Technology Division, National Dairy Research Institute, Karnal 132001, India
Ashish K Singh
Affiliation:
Dairy Technology Division, National Dairy Research Institute, Karnal 132001, India
*
*For correspondence; e-mail: sudhirndri@gmail.com
Get access Rights & Permissions [Opens in a new window]

Abstract

This study was conducted to assess the effect of prebiotic galactooligosaccharides (GOS) on alloxan-induced diabetes in male Sprague-Dawley (SD) rats. Diabetes was induced by administration of alloxan (100 mg/kg) and rats were divided in 4 groups: normal control group (NCG), prebiotic control group (PCG), diabetic control group (DCG) and diabetic prebiotic group (DPG). While PCG and DPG were fed with GOS supplemented (10% w/w) diet, NCG and DCG were administered with basal diet. Rats were sacrificed after 42 d for collection of blood and liver. Faecal samples were collected at the interval of 7 d throughout the study for measurement of lactobacilli and coliform count. Feeding of GOS decreased or delayed the severity of diabetes by amelioration of diabetes associated markers including fasting blood glucose, haemoglobin, glycosylated haemoglobin triglycerides, total cholesterol, low density lipoproteins, creatinine and urea. GOS was also found to improve the levels of antioxidative enzymes (superoxide dismutase, catalase and glutathione peroxidase) in liver and blood. Improvement in lactobacilli count along with a concomitant decrease in coliform count was observed in GOS fed groups.

Keywords

Galactooligosaccharidesdiabetesprebioticshyperglycaemiaoxidative stress
Type
Research Article
Information
Journal of Dairy Research , Volume 82 , Issue 1 , February 2015 , pp. 70 - 77
Copyright
Copyright © Proprietors of Journal of Dairy Research 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aebi, H 1984 Catalase in vitro. In Methods in Enzymology, pp. 121–126 (Eds Colowick, SP & Kaplan, NO). New York: Academic PressGoogle Scholar
Brubake, PL & Drucker, DJ 2004 Minireview: glucagon-like peptides regulate cell proliferation and apoptosis in the pancreas, gut, and central nervous system. Endocrinology 45 2653–2659Google Scholar
Busserolles, J, Gueux, E, Rock, E, Demigne, C, Mazur, A & Rayssiguier, Y 2003 Oligofructose protects against the hypertriglyceridemic and pro-oxidative effects of a high fructose diet in rats. Journal of Nutrition 133 1903–1908Google Scholar
Byung-Sung, P 2011 Effect of oral administration of Jerusalem artichoke inulin on reducing blood lipid and glucose in STZ-induced diabetic rats. Journal of Animal and Veterinary Advances 10 2501–2507Google Scholar
Chen, H, Li-jun, L, Jian-jun, Z, Xua, B & Lib, R 2010 Effect of soybean oligosaccharides on blood lipid, glucose levels and antioxidant enzymes activity in high fat rats. Food Chemistry 119 1633–1636CrossRefGoogle Scholar
Domingues, C, Ruiz, E, Gussinye, M & Carrascosa, A 1998 Oxidative stress at onset and in early stages of type I Diabetes in children and adolescents. Diabetes Care 21 1736–1742Google Scholar
Drabkin, DL 1950 Hydration of macro sized crystals of human hemoglobin, and osmotic concentrations in red cells. The Journal of Biological Chemistry 185 231–245CrossRefGoogle ScholarPubMed
Figueroa-Gonzalez, I, Quijano, G, Ramirez, G & Cruz-Guerrero, A 2011 Probiotics and prebiotics: perspectives and challenges. Journal of the Science of Food and Agriculture 91 1341–1348CrossRefGoogle ScholarPubMed
Fiordaliso, M, Kok, N, Desager, JP, Goethals, F, Deboyser, D, Roberfroid, M & Delzenne, N 1995 Dietary oligofructose lowers triglycerides, phospholipids and cholesterol in serum and very low-density lipoproteins of rats. Lipids 30 163–167Google Scholar
Gobinath, D, Madhu, AN, Prashant, G, Srinivasan, K & Prapulla, SG 2010 Beneficial effect of xylo-oligosaccharides and fructo-oligosaccharides in streptozotocin-induced diabetic rats. British Journal of Nutrition 104 40–47CrossRefGoogle ScholarPubMed
Hsu, CK, Liao, JW, Chung, YC, Hsieh, CP & Chan, YC 2004 Xylooligosaccharides and fructooligosaccharides affect the intestinal microbiota and precancerous colonic lesion development in rats. Journal of Nutrition 134 1523–1528Google Scholar
Imaizumi, K, Nakatsu, Y, Sato, M, Sedarnawati, Y & Sugano, M 1991 Effect of xylooligosaccharides on blood glucose, serum and liver lipid and cecal short chain fatty acid on diabetic rats. Agricultural Biology and Chemistry 55 199–205Google Scholar
Kang, JH, Yun, SI & Park, HO 2010 Effects of Lactobacillus gasseri BNR17 on body weight and adipose tissue mass in diet-induced overweight rats. The Journal of Microbiology 48 712–714CrossRefGoogle ScholarPubMed
Kiebling, G, Schneider, J & Jahreis, G 2002 Long-term consumption of fermented dairy products over 6 months increases HDL cholesterol. European Journal of Clinical Nutrition 56 843–849Google Scholar
Kim, MH & Shin, HK 1998 The water-soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and faecal lipid excretion in rats. Journal of Nutrition 128 1731–1736Google Scholar
Lawrence, RA & Burk, RF 1976 Glutathione peroxidase activity in selenium deficient rat liver. Biochemical and Biophysical Research Communications 71 952–956CrossRefGoogle ScholarPubMed
Liong, MT, Dunshea, FR & Shah, NP 2007 Effects of a synbiotic containing Lactobacillus acidophilus ATCC 4962 on plasma lipid profiles and morphology of erythrocytes in hypercholesterolemic pigs on high- and low-fat diets. British Journal of Nutrition 98 736–744Google Scholar
Luo, J, Van Yperselle, M, Rizkalla, SW, Rossi, F, Bornet, FR & Slama, G 2000 Chronic consumption of short-chain fructooligosaccharides does not affect basal hepatic glucose production or insulin resistance in type 2 diabetics. Journal of Nutrition 130 1572–1577CrossRefGoogle ScholarPubMed
Mabel, MJ, Sangeetha, PT, Platel, K, Srinivasan, K & Prapulla, SG 2008 Physiochemical characterization of fructooligosaccharides and evaluation of their suitability as a potential sweetener for diabetes. Carbohydrate Research 343 56–66CrossRefGoogle Scholar
Marklund, S & Marklund, G 1974 Involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47 469–474CrossRefGoogle Scholar
Moroti, C, Magri, LFS, de Rezende Costa, M, Cavallini, DCU & Sivieri, K 2012 Effect of the consumption of a new symbiotic shake on glycemia and cholesterol levels in elderly people with type 2 diabetes mellitus. Lipids in Health and Disease 11 29CrossRefGoogle ScholarPubMed
Naito, E, Yoshida, Y & Makino, K 2011 Beneficial effect of oral administration of Lactobacillus casei strain Shirota on insulin resistance in diet-induced obesity mice. Journal of Applied Microbiology 110 650–657CrossRefGoogle ScholarPubMed
Nirmala, A, Saroja, S & Gayathri, G 2011 Antidiabetic activity of Basella rubra and its relationship with the antioxidant property. British Biotechnology Journal 1 1–9CrossRefGoogle Scholar
Ohr, LM 2010 Health benefits of probiotics and prebiotics. Food Technology 64 59–64Google Scholar
Qujeq, D & Rezvani, T 2007 Catalase (antioxidant enzyme) activity in streptozotocin-induced diabetic rats. International Journal of Diabetes and Metabolism 15 22–24Google Scholar
Sangwan, V, Tomar, SK, Singh, RRB & Ali, B 2011 Galactooligosaccharides: novel components of designer foods. Journal of Food Science 76 R103–R111CrossRefGoogle ScholarPubMed
Searle, LEJ, Best, A, Nunez, A, Salguero, FJ, Johnson, L, Weyer, U, Dugdale, AH, Cooley, WA, Carter, B, Jones, G, Tzortzis, G, Woodward, MJ & La Ragione, RM 2009 A mixture containing galactooligosaccharide, produced by the enzymic activity of Bifidobacterium bifidum, reduces Salmonella enterica serovar Typhimurium infection in mice. Journal of Medical Microbiology 58 37–48Google Scholar
Shanmugasundaram, R, Devi, KV, Soris, TP, Maruthupandian, A & Mohan, VR 2011 Antidiabetic, antihyperlipidaemic and antioxidant activity of Senna auriculata (L.) roxb leaves in alloxan induced diabetic rats. International Journal of Pharm Tech Research 3 747–756Google Scholar
Sheu, WHH, Lee, IT, Chen, W & Chan, YC 2008 Effects of xylooligosaccharides on type 2 diabetes mellitus. Journal of Nutritional Science and Vitaminology 54 396–401Google Scholar
Stoppa, GR, Cesquini, M, Roman, EAFR, Ogo, SH & Torsoni, MA 2006 Aminoguanidine prevented impairment of blood antioxidant system in insulin-dependent diabetic rats. Life Sciences 78 1352–1361Google Scholar
Swennen, K, Courtin, CM & Delcour, J 2006 Non-digestible oligosaccharides with prebiotic properties. Critical Reviews in Food Science and Nutrition 46 459–471Google Scholar
Szaleczky, E, Prechl, J, Feher, J & Somogyi, A 1999 Alterations in enzymatic antioxidant defence in diabetes mellitus – a rational approach. Postgraduate Medical Journal 75 13–17CrossRefGoogle ScholarPubMed
Tzortzis, G & Vulevic, J 2009 Galacto-oligosaccharide prebiotics. In Prebiotics and Probiotics Science and Technology, pp. 207–244 (Eds Charalampopoulos, D & Rastall, RA). New York: SpringerGoogle Scholar
Valladares, R, Sankar, D, Li, N, Williams, E, Lai, K, Abdelgeliel, AS, Gonzalez, CF, Wasserfall, CH, Larkin, J, Schatz, D, Atkinson, MA, Triplett, EW, Neu, J & Lorca, GL 2010 Lactobacillus johnsonii N6.2 mitigates the development of Type 1 Diabetes in BB-DP Rats. PLoS ONE 5 e10507Google Scholar
Van Bennekum, AM, Nguyen, DV, Schulthess, G, Hauser, H & Phillips, MC 2005 Mechanism of cholesterol-lowering effects of dietary fibers: relationship with intestinal and hepatic cholesterol parameters. British Journal of Nutrition 94 331–337CrossRefGoogle ScholarPubMed
Wang, Y 2009 Prebiotics: present and future in food science and technology. Food Research International 42 8–12Google Scholar
West, E, Simon, OR & Morrison, EY 1996 Streptozotocin alters pancreatic beta-cell responsiveness to glucose within six hours of injection into rats. West Indian Medical Journal 45 60–62Google Scholar
Whiting, DR, Guariguanta, L, Weil, C & Shaw, J 2011 Diabetes Atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Research and Clinical Practice 94 311–321Google Scholar
Yadav, H, Jain, S & Sinha, PR 2008 The effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei on gastropathic consequences in Diabetic rats. Journal of Medicinal Food 11 62–68Google Scholar
Younes, H, Garleb, K, Behr, S, Remesy, C & Demigne, C 1995 Fermentable fibers or oligosaccharides reduce urinary nitrogen excretion by increasing urea disposal in the rat cecum. Journal of Nutrition 125 1010–1016Google ScholarPubMed