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Hormonal induction of α–lactalbumin and β–lactoglobulin in cultured mammary explants from pregnant pigs

  • Susan C. Dodd (a1) (a2), Isabel A. Forsyth (a3), Hugh L. Buttle (a1), Michael I. Gurr (a2) and Raymond R. Dils (a4)...

Summary

Mammary tissue from pigs on days 60, 80, 90, 100 and 100+ (days 106–111) of pregnancy has been cultured in vitro as explants. The total accumulation in tissue and culture medium of the whey proteins α-lactalbumin and β-lactoglobulin has been measured using specific radioimmunoassays. The control, uncultured tissue showed progressive morphological development from sparse, non-secretory epithelial tissue on day 60 to full lobulo-alveolar development with some accumulated secretion from day 100. In uncultured explants β-lactoglobulin could be detected consistently from day 90 (13 ± 12 ng/μg DNA, n = 4) and α-lactalbumin from day 100 (1·3 ± 0·5 ng/μg DNA, n = 11). At all stages of pregnancy, both whey proteins increased markedly during the period of culture (up to 7 d). Stimulation of α-lactalbumin appeared to be primarily under prolactin control. Prolactin increased α-lactalbumin accumulation to a similar extent alone, or in the presence of insulin and/or corticosterone. The response to prolactin was dose-dependent over the range 0·4–20 nM (10–500 ng/ml). Porcine prolactin was more potent than ovine prolactin. There was no effect of porcine growth hormone and no synergism detected between prolactin and tri-iodothyronine. By contrast, no specific hormonal requirements were established for accumulation of β-lactoglobulin, which appeared to increase in vitro if tissue remained viable in various combinations of insulin, corticosterone and prolactin. It was not stimulated by growth hormone. There was some indication of a prolactin-sensitive component in longer term cultures after day 4.

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Ali, S. & Clark, A. J. 1988 Characterization of the gene encoding ovine beta-lactoglobulin: similarity to the genes for retinol binding protein and other secretory proteins. Journal of Molecular Biology 199 415426
Bindal, R. D., Carlson, K. E., Katzenellenbogen, B. S. & Katzenellenbogen, J. A. 1988 Lipophilic impurities, not phenolsulfonphthalein, account for the estrogenic activity in commercial preparations of phenol red. Journal of Steroid Biochemistry 31 287293
Buttle, H. L. 1987 Some effects of total mastectomy upon subsequent reproductive performance in sows. British Veterinary Journal 143 318327
Buttle, H. L. 1988 Role of ovaries in inducing mammogenesis in pregnant pigs. Journal of Endocrinology 118 4145
Collet, C., Joseph, R. & Nicholas, K. 1990 Cloning, cDNA analysis and prolactin-dependent expression of a marsupial α-lactalbumin. Reproduction, Fertility and Development 1 693701
Cowie, A. T., Hartmann, P. E. & Turvey, A. 1969 The maintenance of lactation in the rabbit after hypophysectomy. Journal of Endocrinology 43 651662
Devinoy, E., Houdebine, L.-M. & Delouis, C. 1978 Role of prolactin and glucocorticoids in the expression of casein genes in rabbit mammary gland organ culture. Quantification of casein mRNA. Biochimica et Biophysica Acta 517 360366
Dils, R. R. & Forsyth, I. A. 1981 Preparation and culture of mammary gland explants. Methods in Enzymology 72 724742
Dodd, S. C., Forsyth, I. A., Buttle, H. L., Gurr, M. I. & Dils, R. R. 1994 Milk whey proteins in plasma of sows: variation with physiological state. Journal of Dairy Research 61 2134
Gertler, A., Cohen, N. & Maoz, A. 1983 Human growth hormone but not ovine or bovine growth hormones exhibits galactopoietic prolactin-like activity in organ culture from bovine lautating mammary gland. Molecular and Cellular Endocrinology 33 169182
Godovac-Zimmermann, J. 1988 The structural motif of β-lactoglobulin and retinol binding protein: a basic framework for binding and transport of small Hydrophobic molecules? Trends in Biochemical Sciences 13 6466
Hartmann, P. E., Whitely, J. L. & Willcox, D. L. 1984 Lactose in plasma during lactogenesis, established lactation and weaning in sows. Journal of Physiology 347 453463
Hinegardner, R. T. 1971 An improved fluorometric assay for DNA. Analytical Biochemistry 39 197201
Ichinose, R. R. & Nandi, S. 1966 Influence of hormones on lobulo-alveolar differentiation of mouse mammary glands in vitro. Journal of Endocrinology 35 331340
Jerry, D. J., Griel, L. C., Kavanaugh, J. F. & Kensinger, R. S. 1991 Binding and bioactivity of ovine and porcine prolactins in porcine mammary tissue. Journal of Endocrinology 130 4351
Jerry, D. J., Stover, R. K. & Kensinger, R. S. 1989 Quantitation of prolactin-dependent responses in porcine mammary explants. Journal of Animal Science 67 10131019
Kensinger, R. S., Collier, R. J., Bazer, F. W., Ducsay, C. A. & Becker, H. N. 1982 Nucleic acid, metabolic and histological changes in gilt mammary tissue during pregnancy and lactogenesis. Journal of Animal Science 54 12971308
Kulski, J. K., Topper, Y. J., Chomczynski, P. & Qasba, P. 1983 An essential role for glucocorticoid in casein gene expression in rat mammary explants. Biochemical and Biophysical Research Communications 114 380387
Nicholas, K. R. & Tyndale-Biscoe, C. H. 1985 Prolactin-dependent accumulation of α-lactalbumin in mammary gland explants from the pregnant tammar wallaby (Macropus eugenii). Journal of Endocrinology 106 337342
Plaut, K. I., Kensinger, R. S., Griel, L. C. & Kavanaugh, J. F. 1989 Relationships among prolactin binding, prolactin concentrations in plasma and metabolic activity of the porcine mammary gland. Journal of Animal Science 67 15091519
Puissant, C., Attal, J. & Houdebine, L. M. 1990 The hormonal control of ovine β-lactoglobulin gene in cultured mammary explants from ewes. Reproduction, Nutrition, Développement 30 245251
Sankaran, L. & Topper, Y. J. 1984 Prolactin-induced α-lactalbumin activity in mammary explants from pregnant rabbits. A role for epidermal growth factor and glucocorticoids. Biochemical Journal 217 833837
Shamay, A., Pursel, V. G., Wall, R. J. & Hennighausen, L. 1992 Induction of lactogenesis in transgenic virgin pigs: evidence for gene and integration site-specific hormonal regulation. Molecular Endocrinology 6 191197
Terada, N. & Oka, T. 1982 Selective stimulation of α-lactalbumin synthesis and its mRNA accumulation by thyroid hormone in the differentiation of the mouse mammary gland in vitro. FEBS Letters 149 101104
Vonderhaar, B. K. 1977 Studies on the mechanism by which thyroid hormones enhance α-lactalbumin activity in explants from mouse mammary glands. Endocrinology 100 14231431
Winder, S. J., Turvey, A. & Forsyth, I. A. 1989 Stimulation of UNA synthesis in cultures of ovine mammary epithelial cells by insulin and insulin-like growth factors. Journal of Endocrinology 123 319326

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Hormonal induction of α–lactalbumin and β–lactoglobulin in cultured mammary explants from pregnant pigs

  • Susan C. Dodd (a1) (a2), Isabel A. Forsyth (a3), Hugh L. Buttle (a1), Michael I. Gurr (a2) and Raymond R. Dils (a4)...

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