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Flagella are an important virulence factor in the subclinical persistence of Escherichia coli in bovine mammary gland

Published online by Cambridge University Press:  16 June 2023

Maysa Serpa Gonçalves ,
Jamila PJ Faria ,
Juliana R Silva ,
Dircéia AC Custódio ,
João B Ribeiro ,
Alessandro de S Guimarães ,
Elaine MS Dorneles  and
Geraldo M Costa
Maysa Serpa Gonçalves*
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
Jamila PJ Faria
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
Juliana R Silva
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
Dircéia AC Custódio
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
João B Ribeiro
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Gado de Leite. Av. Eugênio do Nascimento, 610, Aeroporto 36038-330, Juiz de Fora, MG, Brazil
Alessandro de S Guimarães
Affiliation:
Empresa Brasileira de Pesquisa Agropecuária, Embrapa Gado de Leite. Av. Eugênio do Nascimento, 610, Aeroporto 36038-330, Juiz de Fora, MG, Brazil
Elaine MS Dorneles
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
Geraldo M Costa
Affiliation:
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Medicina Veterinária, Universidade Federal de Lavras. Campus Universitário S/N, caixa postal 3037, 37200-900, Lavras, MG, Brazil
*
Corresponding author: Maysa Serpa Gonçalves; Email: maysaserpa@gmail.com
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Abstract

We compared the virulence profile and REP-PCR genotypes of Escherichia coli strains isolated from subclinical and clinical mastitis cases and dairy farm environments in Minas Gerais State, Brazil, to determine virulence factors and genotypes potentially associated with subclinical persistence in the udder. The virulence profile was obtained by the search for three virulence genes: lpfA (long polar fimbriae), fliC (flagella), and escN (type III secretion system). Subclinical isolates exhibited mainly the fliC gene (33.33%) and fliC + escN genes (30.30%). Clinical isolates exhibited mainly fliC + escN genes (50%) and environmental isolates the lpfA + escN genes (58.04%). Strains isolated from subclinical mastitis showed 6.75 times more positivity to fliC than environmental isolates. Thirty-four genotypes were observed in the REP-PCR analysis, and clinical mastitis isolates indicated more genetic proximity to dairy farm environment isolates than subclinical mastitis isolates. In conclusion, the results suggested that flagella may be an important virulence factor for mammary persistent E. coli infection in cattle, however, none of the E. coli REP-PCR genotypes were associated with subclinical infection.

Keywords

Bovine mastitisflagellummammary pathogenic Escherichia coliMPECREP-PCR
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 2 , May 2023 , pp. 146 - 151
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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