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Factors in ruminant colostrum that influence cell growth and murine IgE antibody responses

Published online by Cambridge University Press:  01 June 2009

Dennis L. Watson ,
Geoffrey L. Francis  and
F. John Ballard
Dennis L. Watson
Affiliation:
CSLRO Division of Animal Health, Armidale, New South Wales 2350, Australia
Geoffrey L. Francis
Affiliation:
CSIRO Division of Human Nutrition, Kintore Avenue, Adelaide, South Australia 5000, Australia
F. John Ballard
Affiliation:
CSIRO Division of Human Nutrition, Kintore Avenue, Adelaide, South Australia 5000, Australia
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Summary

Bovine colostrum was investigated as a source of biologically active molecules capable of stimulating the growth of mammalian cells in culture and modifying the immune response in a murine model. An extract prepared from bovine colostral whey by cation exchange and re versed-phase chromatography stimulated the growth of L6 rat myoblasts, Balb/c-3T3 mouse fibroblasts and BHK-21 baby hamster kidney cells with equal or greater potency than fetal bovine serum. Fractionation of the bovine colostral extract by gel-permeation chromatography in M-acetic acid identified a number of cell-growth factors for each cell type. Bovine colostral extract was compared with an ovine colostral whey preparation for its ability to modulate IgE antibody responses in mice. Doses of 8 and 4 mg/d of ovine colostral whey or bovine colostral extract specifically suppressed IgE antibody responses, whereas at lower doses suppression did not occur. We conclude that bovine colostrum contains cell-growth factors as well as immunomodulatory factors that are able to regulate the IgE response in a heterologous species.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 3 , August 1992 , pp. 369 - 380
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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