Hostname: page-component-8448b6f56d-qsmjn Total loading time: 0 Render date: 2024-04-20T01:00:42.577Z Has data issue: false hasContentIssue false
  • English
  • Français

Exogenous phospholipase A2 affects inflammatory gene expression in primary bovine mammary epithelial cells

Published online by Cambridge University Press:  30 April 2019

Jacqueline P. Kurz ,
Mark P. Richards ,
Matthew Garcia  and
Zhongde Wang
Jacqueline P. Kurz
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA Utah Veterinary Diagnostic Laboratory, Utah State University, Logan 84341, USA School of Veterinary Medicine, Utah State University, Logan 84341, USA
Mark P. Richards
Affiliation:
Department of Animal Sciences, Meat Science and Muscle Biology Laboratory, University of Wisconsin-Madison, Madison, WI 53705, USA
Matthew Garcia
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA
Zhongde Wang*
Affiliation:
Department of Animal, Dairy and Veterinary Sciences, Utah State University, Logan 84341, USA
*
Author for correspondence: Zhongde Wang, Email: zonda.wang@usu.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

This Research Communication addresses the hypothesis that exogenously administered phospholipase A2 (PLA2) affects the inflammatory responses of bovine mammary epithelial cells (bMEC) in vitro with the aim of providing preliminary justification of investigation into the uses of exogenously administered PLA2 to manage or treat bovine mastitis. Primary bMEC lines from 11 lactating Holstein dairy cows were established and the expression of 14 pro-inflammatory genes compared under unchallenged and lipopolysaccharide (LPS)-challenged conditions, with and without concurrent treatment with bovine pancreatic PLA2G1B, a secreted form of PLA2. No differences in the expression of these genes were noted between PLA2-treated and untreated bMEC under unchallenged conditions. Following LPS challenge, untreated bMEC exhibited significant downregulation of CXCL8, IL1B, CCL20, and CXCL1. In contrast, PLA2-treated bMEC exhibited significant downregulation of IL1B and CCL20 only. These findings indicate that exogenous PLA2 affects the expression of some pro-inflammatory factors in immune-stimulated bMEC, but does not influence the constitutive expression of these factors. Further investigation of the influence of exogenous PLA2 in the bovine mammary gland is justified.

Keywords

Bovine mammary epithelial cellHolsteinmastitisphospholipase A2
Type
Research Article
Information
Journal of Dairy Research , Volume 86 , Issue 2 , May 2019 , pp. 177 - 180
Copyright
Copyright © Hannah Dairy Research Foundation 2019 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Hanasaki, K (2004) Mammalian phospholipase A2: phospholipase A2 receptor. Biological & Pharmaceutical Bulletin 27, 1165–1167.Google Scholar
Jiang, L, Sorensen, P, Rontved, C, Vels, L and Ingvartsen, K (2008) Gene expression profiling of liver from dairy cows treated intra-mammary with lipopolysaccharide. BMC Genomics 9, 443.Google Scholar
Lahouassa, H, Moussay, E, Rainard, P and Riollet, C (2007) Differential cytokine and chemokine responses of bovine mammary epithelial cells to Staphylococcus aureus and Escherichia coli. Cytokine 38, 12–21.Google Scholar
Lourenço, AG, Komesu, MC, Duarte, G, Del Ciampo, LA, Mussi-Pinhata, MM and Yamamoto, AY (2017) High levels of chemokine C–C motif ligand 20 in human milk and its production by oral keratinocytes. Breastfeeding Medicine 12, 116–121.Google Scholar
Pareek, R, Wellnitz, O, Van Dorp, R, Burton, J and Kerr, D (2005) Immunorelevant gene expression in LPS-challenged bovine mammary epithelial cells. Journal of Applied Genetics 46, 171–177.Google Scholar
Persson Waller, K, Colditz, IG, Lun, S and Östensson, K (2003) Cytokines in mammary lymph and milk during endotoxin-induced bovine mastitis. Research in Veterinary Science 74, 31–36.Google Scholar
Rinaldi, M, Li, RW, Bannerman, DD, Daniels, KM, Evock-Clover, C, Silva, MVB, Paape, MJ, Van Ryssen, B, Burvenich, C and Capuco, AV (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis. Functional and Integrative Genomics 10, 21–38.Google Scholar
Seroussi, E, Klompus, S, Silanikove, M, Krifucks, O, Shapiro, F, Gertler, A and Leitner, G (2013) Nonbactericidal secreted phospholipase A2s are potential anti-inflammatory factors in the mammary gland. Immunogenetics 65, 861–871.Google Scholar
Wellnitz, O, Reith, P, Haas, SC and Meyer, HHD (2006) Immune relevant gene expression of mammary epithelial cells and their influence on leukocyte chemotaxis in response to different mastitis pathogens. Veterinarni Medicina 51, 125–132.Google Scholar
Yedgar, S, Lichtenberg, D and Schnitzer, E (2000) Inhibition of phospholipase A2 as a therapeutic target. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids 1488, 182–187.Google Scholar
Zhang, K, Zhang, D and Jiao, X (2013) full-text. European Review for Medical and Pharmacological Sciences 17, 3279–3284.Google Scholar
Zheng, J, Watson, AD and Kerr, DE (2006) Genome-wide expression analysis of lipopolysaccharide-induced mastitis in a mouse model. Infection and Immunity 74, 1907–1915.Google Scholar
Supplementary material: PDF

Kurz et al. supplementary material

Table S1

Download Kurz et al. supplementary material(PDF)
PDF 100.5 KB