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Evaluation of tRNA intergenic spacer length polymorphism analysis as a molecular method for species identification of streptococcal isolates from bovine mastitis

Published online by Cambridge University Press:  09 May 2005

Michael Zschöck ,
Sabine Manhold-Maurer ,
Agnes Wescher ,
Kristin Merl ,
Ishar Khan  and
Christoph Lämmler
Michael Zschöck
Affiliation:
Staatliches Untersuchungsamt Hessen, Abteilung Veterinärmedizin, Marburger Str. 54, D-35396 Giessen, Germany
Sabine Manhold-Maurer
Affiliation:
Staatliches Untersuchungsamt Hessen, Abteilung Veterinärmedizin, Marburger Str. 54, D-35396 Giessen, Germany
Agnes Wescher
Affiliation:
Staatliches Untersuchungsamt Hessen, Abteilung Veterinärmedizin, Marburger Str. 54, D-35396 Giessen, Germany
Kristin Merl
Affiliation:
Regierungspräsidium Darmstadt, Veterinärdezernat, Luisenplatz 2, D-64283 Darmstadt, Germany
Ishar Khan
Affiliation:
Department of Environmental Health, Toxicology Division, University of Cincinnati, Medical Center, 3223 Eden Ave, Cincinnati OH, USA
Christoph Lämmler
Affiliation:
Institut für Pharmakologie und Toxikologie, Justus-Liebig-Universität Gießen, Frankfurter Str. 107, D-35392 Giessen, Germany
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Abstract

Sixty-nine bovine mastitis streptococci belonging to the species Str. agalactiae (n=13), Str. dysgalactiae (n=16), Str. canis (n=22), Str. uberis (n=20) and Str. parauberis (n=4) and six reference strains of the five streptococcal species were examined for their tRNA gene intergenic length polymorphism (tDNA-ILP) fingerprint pattern. Epidemiologically unrelated isolates from bovine mastitis cases were selected by macrorestriction analysis with pulsed-field gel electrophoresis (PFGE). Their results were compared with those obtained from biochemical and serological studies and with those obtained by PCR-mediated identification amplifying species-specific gene segments of the five streptococcal species. According to the present results tDNA-ILP allowed a correct identification of all Str. agalactiae, Str. uberis and Str. parauberis strains investigated also including the reference strains of each species showing species-specific banding pattern. However, all Str. dysgalactiae ssp. dysgalactiae and all Str. canis strains appeared with an undistinguishable pattern which did not allow an identification of the species.

Keywords

Streptococcus speciesbovine mastitisidentificationtDNA-ILP-PCR
Type
Research Article
Information
Journal of Dairy Research , Volume 72 , Issue 3 , August 2005 , pp. 333 - 337
Copyright
© Proprietors of Journal of Dairy Research 2005

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