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Discovery of an ovine αs2-casein variant

Published online by Cambridge University Press:  01 June 2009

Lina Chianese
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Giuseppina Garro
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Francesco Addeo
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Gloria Lopez-Galvez
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid 28006, España
Mercedes Ramos
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid 28006, España

Summary

A novel ovine αs2-casein variant has been detected using discontinuons polyacrylamide gel electrophoresis at alkaline pH, two dimensional electrophoresis and immunoblotting. It is characterized by a greater negative net charge and a lower isoelectric point compared with the most common ovine αs2-casein variant. The phenotypic frequency in the Manchega breed is 5·5%.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

REFERENCES

Aschaffenburg, R. & Drewby, J. 1959 New procedure for the routine determination of the various non-casein proteins of milk. 15th International Dairy Congress, London 3 16311637Google Scholar
Brignon, G., Mahé, M.-F., Ribadeau-Dumas, B., Mercier, J.-C. & Grosclaude, F. 1990 Two of the three genetic variants of goat αs1-casein which are synthesized at a reduced level have an internal deletion possibly due to altered RNA splicing. European Journal of Biocheinistry 193 237241CrossRefGoogle Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1990 a Heterogeneity of sheep's casein by gel electrophoresis and immunochemical techniques. Brief Communications and Abstracts of Posters of the XXIII International Dairy Congress 1 18Google Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 3947CrossRefGoogle ScholarPubMed
Chianese, L., Mauriello, R., Moio, L., Intorcia, N., Campus, R. & Addeo, F. 1990 b [Casein characterization in the Sarda ovine breed.] Proceedings of the XLIVth SIS Veterinary (Italian Veterinary Society) National Congress, 17011704Google Scholar
Dali'Olio, S., Davoli, R. & Bosi, P. 1989 [Electrophoretic research on milk proteins in milk from ewes of the Sopravissana breed.] Scienza e Tecnica Lattiero-Casearia 40 186194Google Scholar
Dall'Olio, S., Davoli, R. & Russo, V. 1990 Affinity chromatography of ovine cascin. Journal of Dairy Science 73 17071711CrossRefGoogle Scholar
Davoli, R., Dall'Olio, S. & Russo, V. 1990 Evidence supporting attribution of ovine “Welsh” variant to αs1-casein. Scienza e Tecnica Lattiero-Casearia 41 Suppl. 327333Google Scholar
Di Luccia, A., Iannibelli, L., Ferrara, L., Ledda, L., Moio, L., Pieragostini, E. & Addeo, F. 1989 Identification of Welsh variant from ovine casein by electrophoresis techniques. In Electrophoresis Forum '89 pp. 394397 (Ed. Radola, B. J.). MünchenGoogle Scholar
King, J. W. B. 1967 The caseins of sheep's milk. In Polymorphismes Biochimiques des Animaux pp. 427431. Paris: INRA (European Conference on Animal Blood Groups and Biochemical Polymorphisms 10 1966)Google Scholar
Pieragostini, E., Chianese, L., Mauriello, R. & D'Acierno, G. 1989 [Ovine casein polymorphism in Leccese breed.] Proceedings of the XLIII SIS Veterinary (Italian Veterinary Society) National Congress, Pisa, 18751879Google Scholar
Thompson, M. P., Gordon, W. G., Boswell, R. T. & Farrell, H. M. 1969 Solubility, solvation and stabilization of αs1- and β–caseins. Journal of Dairy Science 52 11661173CrossRefGoogle Scholar