Hostname: page-component-77c89778f8-m42fx Total loading time: 0 Render date: 2024-07-22T03:36:26.788Z Has data issue: false hasContentIssue false
  • English
  • Français

Discovery of an ovine αs2-casein variant

Published online by Cambridge University Press:  01 June 2009

Lina Chianese ,
Giuseppina Garro ,
Francesco Addeo ,
Gloria Lopez-Galvez  and
Mercedes Ramos
Lina Chianese
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Giuseppina Garro
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Francesco Addeo
Affiliation:
Istituto di Industrie Agrarie, Facoltà di Agraria dell'Università degli Studi di Napoli Federico II, 80055 Portici, Italia
Gloria Lopez-Galvez
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid 28006, España
Mercedes Ramos
Affiliation:
Instituto de Fermentaciones Industriales (CSIC), Madrid 28006, España
Get access Rights & Permissions [Opens in a new window]

Summary

A novel ovine αs2-casein variant has been detected using discontinuons polyacrylamide gel electrophoresis at alkaline pH, two dimensional electrophoresis and immunoblotting. It is characterized by a greater negative net charge and a lower isoelectric point compared with the most common ovine αs2-casein variant. The phenotypic frequency in the Manchega breed is 5·5%.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 4 , November 1993 , pp. 485 - 493
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Aschaffenburg, R. & Drewby, J. 1959 New procedure for the routine determination of the various non-casein proteins of milk. 15th International Dairy Congress, London 3 1631–1637Google Scholar
Brignon, G., Mahé, M.-F., Ribadeau-Dumas, B., Mercier, J.-C. & Grosclaude, F. 1990 Two of the three genetic variants of goat αs1-casein which are synthesized at a reduced level have an internal deletion possibly due to altered RNA splicing. European Journal of Biocheinistry 193 237–241CrossRefGoogle Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1990 a Heterogeneity of sheep's casein by gel electrophoresis and immunochemical techniques. Brief Communications and Abstracts of Posters of the XXIII International Dairy Congress 1 18Google Scholar
Chianese, L., Mauriello, R., Moio, L., Intorcia, N. & Addeo, F. 1992 Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques. Journal of Dairy Research 59 39–47CrossRefGoogle ScholarPubMed
Chianese, L., Mauriello, R., Moio, L., Intorcia, N., Campus, R. & Addeo, F. 1990 b [Casein characterization in the Sarda ovine breed.] Proceedings of the XLIVth SIS Veterinary (Italian Veterinary Society) National Congress, 1701–1704Google Scholar
Dali'Olio, S., Davoli, R. & Bosi, P. 1989 [Electrophoretic research on milk proteins in milk from ewes of the Sopravissana breed.] Scienza e Tecnica Lattiero-Casearia 40 186–194Google Scholar
Dall'Olio, S., Davoli, R. & Russo, V. 1990 Affinity chromatography of ovine cascin. Journal of Dairy Science 73 1707–1711CrossRefGoogle Scholar
Davoli, R., Dall'Olio, S. & Russo, V. 1990 Evidence supporting attribution of ovine “Welsh” variant to αs1-casein. Scienza e Tecnica Lattiero-Casearia 41 Suppl. 327–333Google Scholar
Di Luccia, A., Iannibelli, L., Ferrara, L., Ledda, L., Moio, L., Pieragostini, E. & Addeo, F. 1989 Identification of Welsh variant from ovine casein by electrophoresis techniques. In Electrophoresis Forum '89 pp. 394–397 (Ed. Radola, B. J.). MünchenGoogle Scholar
King, J. W. B. 1967 The caseins of sheep's milk. In Polymorphismes Biochimiques des Animaux pp. 427–431. Paris: INRA (European Conference on Animal Blood Groups and Biochemical Polymorphisms 10 1966)Google Scholar
Pieragostini, E., Chianese, L., Mauriello, R. & D'Acierno, G. 1989 [Ovine casein polymorphism in Leccese breed.] Proceedings of the XLIII SIS Veterinary (Italian Veterinary Society) National Congress, Pisa, 1875–1879Google Scholar
Thompson, M. P., Gordon, W. G., Boswell, R. T. & Farrell, H. M. 1969 Solubility, solvation and stabilization of αs1- and β–caseins. Journal of Dairy Science 52 1166–1173CrossRefGoogle Scholar