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Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques

  • Lina Chianese (a1), Rosalba Mauriello (a1), Luigi Moio (a1), Nunziatina Intorcia (a1) and Francesco Addeo (a1)...


Discontinuous PAGE at alkaline and acid pH, polyacrylamide gel isoelectric focusing, two dimensional electrophoresis and immunoblotting have been used to study the heterogeneity of sheep caseins. Three main phenotypes were selected either because of mobility at alkaline and acid pH of the individual αs components or because of their relative intensity. On electrophoresis at alkaline pH, one phenotype showed two distinct bands of lower electrophoretic mobility than β,- and β2-caseins. A detailed study of these components using immunospecific detection with β-casein antiserum showed that these minor components of ovine casein may have a polypeptide chain similar to that of β1- and β2-caseins. Complete electrophoretic patterns of the casein components in some individual milks are also presented.



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Addeo, F., Martin, P. & Ribadeau-Dumas, B. 1984 Susceptibility of buffalo and cow κ-caseins to chymosin action. Milchwissenschaft 39 202205
Addeo, F., Masi, P. & Rubino, R. 1989 The aptitude of caprine milk to cheese making. Ilelationship between αs1-casein content and rheological properties of curd and cheese. Proceedings of Symposium ‘Philostios’, Fonte-Boa, 1987 Agriculture (Eurostat) pp. 291311
Addeo, F., Mauriello, R. & Di Luccia, A. 1988 A gel electrophoretic study of caprine casein. Journal of Dairy Research 55 413421
Addeo, F., Moio, L., Chianese, L., Stingo, C., Resmini, P., Berner, I., Krause, T., Di Luccia, A. & Bocca, A. 1990 Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine cheese by gel isoelectric focusing of γ2-caseins. Milchwissenschaft 45 708711
Arave, C. W., Gillett, T. A., Price, D. A. & Matthews, D. H. 1973. Polymorphisms in caseins of sheep milk. Journal of Animal Science 36 241244
Aschaffenburg, R. & Drewry, J. 1959 New procedure for the routine determination of the varions noncasein proteins of milk. 15th International Dairy Congress, London 3 16311637
Beisiegel, U. 1986 Protein blotting. (Review) Electrophoresis 7 118
Blakesley, R. W. & Boezi, J. A. 1977 A new staining technique for proteins in polyacrylamide gels using Coomassie Brilliant Blue G-250. Analytical Biochemislry 92 580582
Caroli, A., Bolla, P., Pagnacco, G. & Fraghi, A. 1990 Study on the genetic control of the Welsh phenotype of sheep αs-casein. Proceedings of the XXIVth International Symposium of Zootecnia 177179
Chianese, L., Mauriello, R., Moio, L., Intorcia, N., Campus, R. & Addeo, F. 1990. [Casein characterization in the Sarda ovine race.] Proceedings of the XLIVth S.I.S. Vet. (Italian Veterinary Science Society) National Congress, Stresa, 1990 In press
Di Luccia, A., Iannibelli, L., Ferrara, L., Ledda, L., Moio, L., Pieragostini, E. & Addeo, F. 1989 Identification of Welsh variant from ovine casein by electrophoresis techniques. In Electrophoresis Forum '89 pp. 394397 (Ed. Radola, B. J.). München
Haasnoot, W., Venema, D. P. & Elenbaas, H. L. 1986 Determination of cow milk in the milk and cheese of ewes and goats by fast protein liquid chromatography. Milchwissenchaft 41 642645
King, J. W. B. 1967 The caseins of sheep's milk. In Polymorphismes Biochimiques des Animaux pp. 427431. Paris: INRA (European Conference on Animal Blood Groups and Biochemical Polymorphisms 10 1966)
Krause, I. & Belitz, H. D. 1985 [Differentiation of the milk proteins of various animals: detection of cows' milk in cheese made from sheep, goat and buffalo milk.] Lebensmittetchemie und Gerichtliche Chemie 39 3336
Mauriello, R., Pieragostini, E., Bufano, G. & Addeo, F. 1990 [Polymorphism of caseins in the Altamurana sheep.] Scienza e Tecnica Lattiero-Casearia 41 357364
Mercier, J. C., Maubois, J.-L., Poznanski, S. & Ribadeau-Dumas, B. 1968 [Preparative fractionation of bovine and ovine casein by chromatography on DEAE-cellulose with urea and 2-inercaptoethanol.] Bulletin de la Société de Chimie Biologique 50 521530
Moio, L., Chinaese, L., Di Luccia, A., Mauriello, R., Stingo, C. & Addeo, F. 1989 Identification of casein variants by immunoblotting. In Electrophoresis Forum '89 pp. 475478 (Ed. Radola, B. J.). München
Pieragostini, E., Chianese, L., Mauriello, R. & D'Acierno, C. 1989 [Polymorphism of caseins in Leccese sheep]. Proceedings of the XLIIIrd S.I.S. Vet. (Italian Veterinary Science Society) National Congress, Pisa pp. 18751879
Richardson, B. C. & Creamer, L. K. 1976 Comparative micelle structure. 5. The isolation and characterization of the major ovine caseins. New Zealand Journal of Dairy Science and Technology 11 4653
Russo, V., Davoli, R., Dall'Olio, S. & Tedeschi, M. 1986 [Research on polymorphism of proteins in goats' and ewes' milk.] Zootecnica e Nutrizione Animale 12 5562
Trieu-Cuot, P. & Gripon, J.-C. 1981 Electrofocusing and two-dimensional electrophoresis of bovine caseins. Journal of Dairy Research 48 303310
Tsang, V. C. W., Peralta, J. M. & Simons, A. R. 1983 Enzyme-linked immunoelectrotrasfer blot techniques (E1TB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92 377391

Determination of ovine casein heterogeneity using gel electrophoresis and immunochemical techniques

  • Lina Chianese (a1), Rosalba Mauriello (a1), Luigi Moio (a1), Nunziatina Intorcia (a1) and Francesco Addeo (a1)...


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