Skip to main content Accessibility help
×
Home

Comparison of the ability to bind lipids of β-lactoglobulin and serum albumin of milk from ruminant and non-ruminant species

  • M. Dolores Pérez (a1), Pilar Puyol (a1), José Manuel Ena (a1) and Miguel Calvo (a1)

Summary

The interaction of sheep, horse, pig, human and guinea-pig whey proteins with fatty acids has been studied. Using gel filtration and autoradiography, it was found that sheep β-lactoglobulin and serum albumin from all species had the ability to bind fatty acids in vitro. Sheep β-lactoglobulin, isolated from milk, had ˜ 0·5 mol fatty acids bound per mol monomer protein, and albumin from sheep, horse and pig contained ˜ 4·5, 2·9 and 4·7 mol fatty acids/mol protein respectively. However, β-lactoglobulin from horse and pig milk had neither fatty acids physiologically bound nor the ability to bind them in vitro. Albumin was the only whey protein detected with bound fatty acids in these species as well as in human and guinea pig. This suggests that the ability of ruminant β-lactoglobulin to bind fatty acids was not shared by the same protein of non-ruminants.

Copyright

Corresponding author

*For correspondence.

References

Hide All
Bell, K., McKenzie, H. A., Muller, V., Rogers, C. & Shaw, D. C. 1981 a Equine whey proteins. Comparative Biochemistry and Physiology 68B 225236
Bell, K., McKenzin, H. A. & Shaw, D. C. 1981 b Porcine β-lactoglobulin A and C. Molecular and Cellular Biochemistry 35 103111
Bernbäck, S., Hernell, O. & Bläckberg, L. 1987 Bovine pregastric lipase: a model for the human enzyme with respect to properties relevant to its site of action. Biochimica et Biophysica Acta 922 206213
Chen, R. F. 1967 Removal of fatty acids from serum albumin by charcoal treatment. Journal of Biological Chemistry 242 173181
Conti, A., Godovac-Zimmermann, J., Liberatori, J., Braunitzer, G. & Minori, D. 1984 The primary structure of monomeric β-lactoglobulin I from horse colostrum (Equus caballus, Perissodactyla). Hoppe-Seyler's Zeitschrift für Physiologische Chemie 365 13931401
Conti, A., Godovac-Zimmermann, J., Pirchner, F., Liberatori, J. & Braunitzer, G. 1986 Pig β-lactoglobulin I (Sus scrofa domestica, Artiodactyla). Biological Chemistry Hoppe-Seyler 367 871878
Conti, A., Liberatori, J. & Napolitano, N. 1982 Heterogeneity of equine β-lactoglobulins. Milchwissenschaft 37 338340
Creamer, L. K., Parry, D. A. D. & Malcolm, G. N. 1983 Secondary structure of bovine β-lactoglobulin B. Archives of Biochem stry and Biophysics 227 98105
Diaz de Villegas, C., Oria, R., Sala, F. J. & Calvo, M. 1987 Lipid binding by β-lactoglobulin of cow milk. Milchwissenschaft 42 357358
Ena, J. M., Esteban, C., Pérez, M. D., Uriel, J. & Calvo, M. 1989 Fatty acids bound to vitamin D-binding protein (DBP) from human and bovine sera. Biochemistry International 19 17
Ena, J. M., Pérez, M. D., Aranda, P., Sanchez, L. & Calvo, M. 1992 Presence and changes in the concentration of vitamin D-binding protein throughout early lactation in human and bovine colostrum and milk. Journal of Nutritional Biochemistry. In Press
Folch, J., Lees, M. & Sloane Stanley, G. H. 1957 A simple method for the isolation and purification of total lipides from animal tissues. Journal of Biological Chemistry 226 497509
Fugate, R. D. & Song, P.-S. 1980 Spectroscopic characterization of β-lactoglobulin-retinol complex. Biochimica et Biophysica Acta 625 2842
Godovac-Zimmermann, J. & Braunitzer, G. 1987 Modern aspects of the primary structure and function of β-lactoglobulins. Milchwissenschaft 42 294297
Godovac-Zimmermann, J., Conti, A., Liberatori, J. & Braunitzer, G. 1985 Homology between the primary structures of β-lactoglobulins and human retinol-binding protein: evidence for a similar biological function? Biological Chemistry Hoppe-Seyler 366 431434
Halliday, J. A., Bell, K. & Shaw, D. C. 1991 The complete amino acid sequence of feline β-lactoglobulin II and a partial revision of the equine β-lactoglobulin II sequence. Biochimica et Biophysica Acta 1077 2530
Hamosh, M., Scanlon, J. W., Ganot, D., Likel, M., Scanlon, K. B. & Hamosh, P. 1981 Fat digestion in newborn. Characterization of lipase in gastric aspirates of premature and term infants. Journal of Clinical Investigation 67 838846
Kalan, E. B., Kraeling, R. R. & Gerrits, R. J. 1971 Isolation and partial characterization of a polymorphic swine whey protein. International Journal of Biochemistry 2 232244
Kessler, E. & Brew, K. 1970 The whey proteins of pig's milk. Isolation and characterization of a β-lactoglobulin. Biochimica et Biophysica Acta 200 449458
Kolde, H. J. & Braunitzer, G. 1983 The primary structure of ovine β-lactoglobulin. 2. Discussion and genetic aspects. Milchwissenschaft 38 7072
Lampreave, F., Calvo, M., Naval, J. & Piñeiro, A. 1982 Long-chain fatty acids bound to α-fetoprotein and to serum albumin from fetal and adult pig. Comparative Biochemistry and Physiology 73B 823827
Laurell, C.-B. 1966 Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Analytical Biochemistry 15 4552
Lindström, M. B., Persson, J., Thurn, L. & Borgström, B. 1991 Effect of pancreatic phospholipase A2 and gastric lipase on the action of pancreatic carboxyl ester lipase against lipid substrates in vitro. Biochimica et Biophysica Acta 1084 194197
Moreau, H., Gargoury, Y., Lecat, D., Junien, J. L. & Verger, R. 1988 Screening of preduodenal lipases in several mammals. Biochimica et Biophysica Acta 959 247252
Pérez, M. D., Díaz de Villegas, C., Sánchez, L., Aranda, P., Ena, J. M. & Calvo, M. 1989 Interaction of fatty acids with β-lactoglobulin and albumin from ruminant milk. Journal of Biochemistry 106 10941097
Pérez, M. D., Sanchez, L., Aranda, P., Ena, J. M., Oria, R. & Calvo, M. 1990 Synthesis and evolution of concentration of β-lactoglobulin and α-lactalbumin from cow and sheep colostrum and milk throughout early lactation. Cellular and Molecular Biology 36 205212
Pérez, M. D., Sanchez, L., Aranda, P., Ena, J. M., Oria, R. & Calvo, M. 1992 Effect of β-lactoglobulin on the activity of pregastric lipase. A possible role for this protein in ruminant milk. Biochimica et Biophysica Acta 1123 151155
Puyol, P., Pérez, M. D., Ena, J. M. & Calvo, M. 1991 Interaction of bovine β-lactoglobulin and other bovine and human whey proteins with retinol and fatty acids. Agricultural and Biological Chemistry 55 25152520
Said, H. M., Ong, D. E. & Shingleton, J. L. 1989 Intestinal uptake of retinol: enhancement by bovine milk β-lactoglobulin. American Journal of Clinical Nutrition 49 690694
Spector, A. A. & Fletcher, J. E. 1970 Binding of long chain fatty acids to β-lactoglobulin. Lipids 5 403411
Uriel, J. 1966 [Electrophoresis in acrylamide-agarose gels.] BulLetin de la Société Chimie Biologique 48 969982
Wichman, A. & Andersson, L.-O. 1974 Purification of human serum albumin by affinity chromatography. Biochimica et Biophysica Acta 372 218224

Comparison of the ability to bind lipids of β-lactoglobulin and serum albumin of milk from ruminant and non-ruminant species

  • M. Dolores Pérez (a1), Pilar Puyol (a1), José Manuel Ena (a1) and Miguel Calvo (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed