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Characteristics of ruminant mammary epithelial cells grown in primary culture in serum-free medium

Published online by Cambridge University Press:  01 June 2009

Steven J. Winder ,
Alan Turvey  and
Isabel A. Forsyth
Steven J. Winder
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal Production, Hurley, Maidenhead, Berks SL6 5LR, UK
Alan Turvey
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal Production, Hurley, Maidenhead, Berks SL6 5LR, UK
Isabel A. Forsyth
Affiliation:
Endocrinology and Animal Physiology Department, AFRC Institute for Grassland and Animal Production, Hurley, Maidenhead, Berks SL6 5LR, UK
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Summary

Cells were obtained from the mammary glands of sheep and cows by collagenase–hyaluronidase digestion. Characterization of cells as epithelial was by reaction with a monoclonal antibody to cytokeratin. A subpopulation of spindle-shaped or stellate cells reacted with a monoclonal antibody to desmin and may be related to myoepithelial cells. The development is described of a simple serum-free culture system for these cells on gels of rat tail (type 1) collagen. A commercial medium (M199) was used, buffered with Hepes and with bovine serum albumin as the sole protein supplement, plus fibronectin for the first 18 h only as an attachment factor. The cell cultures showed stimulated DNA synthesis in response to mitogens on attached gels and also responded as floating cultures to lactogenic hormones with production of α-lactalbumin.

Type
Original Articles
Information
Journal of Dairy Research , Volume 59 , Issue 4 , November 1992 , pp. 491 - 498
Copyright
Copyright © Proprietors of Journal of Dairy Research 1992

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References

REFERENCES

Emerman, J. T. & Pitelka, D. R. 1977 Maintenance and induction of morphological differentiation in dissociated mammary epithelium on floating collagen membranes. In Vitro 13 316–328CrossRefGoogle ScholarPubMed
Ethier, S. P., Kudla, A. & Cundiff, K. C. 1987 Influence of hormone and growth factor interactions on the proliferative potential of normal rat mammary epithelial cells in vitro. Journal of Cellular Physiology 132 161–167CrossRefGoogle ScholarPubMed
Forsyth, I. A., Byatt, J. C. & Ilev, S. 1985 Hormone concentrations, mammary development and milk yield in goats given long-term bromocriptine treatment in pregnancy. Journal of Endocrinology 104 7785Google Scholar
Hammond, S. L., Ham, R. G. & Stampfer, M. R. 1984 Serum-free growth of human mammary epithelial cells: rapid donai growth in defined medium and extended serial passage with pituitary extract. Proceedings of the National Academy of Sciences of the USA 81 5435–5439CrossRefGoogle Scholar
Hansen, H. O. & Knudsen, J. 1991 Lactating goat mammary gland cells in culture. Comparative Biochemistry & Physiology 99A 129–135CrossRefGoogle Scholar
Imagawa, W., Bandyopadhyay, G. K. & Nandi, S. 1990 Regulation of mammary epithelial cell growth in mice and rats. Endocrine-Reviews 11 494–523Google Scholar
Imagawa, W., Tomooka, Y. & Nandi, S. 1982 Serum-free growth of normal and tumor mouse mammary epithelial cells in primary culture. Proceedings of the National Academy of Sciences of the USA 79 4074–4077Google Scholar
Kraehenbuhl, J. P. 1977 Dispersed mammary gland epithelial cells. I. Isolation and separation procedures. Journal of Cell Biology 72 390–405CrossRefGoogle ScholarPubMed
Lippman, M. E. & Dickson, R. B. 1989 Mechanisms of growth control in normal and malignant breast epithelium, Recent Progress in Hormone Research 45 383–435Google Scholar
McGrath, M. F. 1987 A novel system for mammary epithelial cell culture. Journal of Dairy Science 70 1907–1980CrossRefGoogle ScholarPubMed
McGrath, M. F., Collier, R. J., Clemmons, D. R., Busby, W. H., Sweeny, C. A. & Krivi, G. G. 1991 The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins. Endocrinology 129 071–678Google Scholar
Mackenzie, D. D. S., Brooker, B. E. & Forsyth, I. A. 1985 Ultrastructural features of bovine mammary epithelial cells grown on collagen gels. Tissue & Cell 17 39–51Google Scholar
Mackenzie, D. D. S., Forsyth, I. A., Brooker, B. E. & Turvey, A. 1982 Culture of bovine mammary epithelial cells on collagen gels. Tissue & Cell 14 231–241Google Scholar
Schmid, E., Schiller, D. L., Grund, C., Stabler, J. & Franke, W. W. 1983 Tissue type-specific expression of intermediate filament proteins in a cultured epithelial cell line from bovine mammary gland. Journal of Cell Biology 96 37–50Google Scholar
Shamay, A., Cohen, N., Niwa, M. & Gertler, A. 1988 Effect of insulin-like growth factor I on deoxyribonucleic acid synthesis and galactopoiesis in bovine undifferentiated and lactating mammary tissue in vitro. Endocrinology 123 804–809Google Scholar
Talhouk, R. S., Neiswander, R. L. & Schanbacher, F. L. 1990 In vitro culture of cryopreserved bovine mammary cells on collagen gels: synthesis and secretion of casein and lactoferrin. Tissue & Cell 22 583–599CrossRefGoogle ScholarPubMed
Wheatley, S. D., Coles, M. L., Turvey, A., Morrell, D. J. & Forsyth, I. A. 1989 Insulin-like growth factor-I release by ovine mammary epithelial cells. Journal of Endocrinology 123. Suppl. Abstr. no. 118Google Scholar
Wheatley, S. D. & Forsyth, I. A. 1990 Both 1GF-II and the truncated form of 1CF-I stimulate DNA synthesis in ovine mammary epithelial cells in vitro. Journal of Reproduction and Fertility, Abstract Series 5 114Google Scholar
Wicha, M. S., Lowrie, G., Kohs, E., Bagavandoss, P. & Mahn, T. 1982 Extracellular matrix promotes mammary epithelial growth and differentiation in vitro. Proceedings of the National Academy of Sciences of the USA 79 3213–3217CrossRefGoogle ScholarPubMed
Winder, S. J., Turvey, A. & Forsyth, I. A. 1989 Stimulation of DNA synthesis in cultures of ovine mammary epithelial cells by insulin and insulin-like growth factors. Journal of Endocrinology 123 319–326CrossRefGoogle ScholarPubMed
Zurfluh, L. L., Bolten, S. L., Byatt, J. C., McGrath, M. F., Tou, J. S., Zupec, M. E. & Krivi, G. G. 1990 Isolation of genomic sequence encoding a biologically active bovine TGF-α protein. Growth Factors 3 257–266CrossRefGoogle ScholarPubMed