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Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes

  • Catherine A. O'Sullivan (a1), Patrick J. Joyce (a1), Teresa Sloan (a1) and Alan G. Shattock (a2)

Summary

A direct capture enzyme-linked immunosorbent assay (ELISA) was developed to measure elevated polymorphonuclear granulocyte (PMN) antigens using horseradish peroxidase (EC 1.11.1.7) conjugated rabbit polyclonal anti-PMN antisera and a monoclonal antibody specific for PMN cells. Optical densities obtained in the ELISA were used to predict the cell counts of milk samples. Predicted counts were not significantly different from actual somatic cell counts (SCC). In a total of 156 bovine milk samples the correlation coefficient between somatic cell counting, taking > 500000 cells/ml as being indicative of mastitis, and the assay was 0·94, yielding an assay sensitivity of 95·2% and a specificity of 97·3%. In further trials the ELISA could detect elevated PMN antigens in milk with SCC as low as 100000 cells/ml. The results indicate that the monoclonal antibody based direct ELISA has excellent potential in the detection and determination of bovine mastitis.

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Campbell, R. C. 1967 Statistics for Biologists. Cambridge: Cambridge University Press
Carlson, G. P. & Kaneko, J. J. 1973 Isolation of leukocytes from bovine peripheral blood. Proceedings of the Society for Experimental Biology and Medicine 142 853856
Cotter, T. G., Spears, P. & Henson, P. M. 1981 A monoclonal antibody inhibiting human neutrophil chemotaxis and degranulation. Journal of Immunology 127 13551360
Cullen, G. A. 1966 Cells in milk. A review. Veterinary Bulletin 36 337346
Eberhart, R. J., Harmon, R. J., Jasper, D. E., Natzke, R. P., Nickerson, S. C., Reneau, J. K., Row, E. H., Smith, K. L. & Spencer, S. B. 1987 In Current Concepts of Bovine Mastitis, 3rd edn.USA: National Mastitis Council
Fernando, R. S., Rindsig, R. B. & Spahr, S. L. 1982 Electrical conductivity of milk for detection of mastitis. Journal of Dairy Science 65 659664
Hart, G. 1980 Screening to control infectious diseases: evaluation of control programs for gonorrhea and syphilis. Reviews of Infectious Diseases 2 701712
Kitchen, B. J. 1981 Review of the progress of Dairy Science: Bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research 48 167188
Kitchen, B. J., Middleton, G., Durward, I. G., Andrews, R. J. & Salmon, M. C. 1980 Mastitis diagnostic tests to estimate mammary gland epithelial cell damage. Journal of Dairy Science 63 978983
Laemmli, U. K. 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 680685
Lightner, J. K., Miller, G. Y., Hueston, W. D. & Dorn, C. R. 1988 Food animal economics: estimation of the costs of mastitis, using National Animal Health Monitoring System and milk somatic cell count data. Journal of the American Veterinary Medical Association 192 14101413
Meek, A. H., Barnum, D. A. & Newbould, F. H. S. 1980 Use of total and differential somatic cell counts to differentiate potentially infected from potentially non-infected quarters and cows and between herds of various levels of infection. Journal of Food Protection 43 1014
Ruffo, G. 1968 The role of the cell count in the diagnosis of chronic staphylococcal mastitis. Industria Latte 4 278287
St Groth, S. F. De & Scheidegger, D. 1980 Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35 121
Schalm, O. W., Carroll, E. J. & Jain, N. C. 1971 Bovine Mastitis. Philadelphia, PA: Lea and Febiger
Schultze, W. D. 1985 Developments in the identification of diseased udder quarters or cows. Kieler Milchwirtschaftliche Forschungsberichte 37 319328
Smith, A. M. & Tedder, R. S. 1981 Development of an enzyme-linked immunosorbent assay for hepatitis Be antigen and antibody. Journal of Virological Methods 3 111
Smith, K. L. 1983 Mastitis control: a discussion. Journal of Dairy Science 66 17901794
Towbin, H., Staehelin, T. & Gordon, J. 1979 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proceedings of the National Academy of Sciences, USA 76 43504354

Capture immunoassay for the diagnosis of bovine mastitis using a monoclonal antibody to polymorphonuclear granulocytes

  • Catherine A. O'Sullivan (a1), Patrick J. Joyce (a1), Teresa Sloan (a1) and Alan G. Shattock (a2)

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