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Biochemical characterization of bovine αs1-casein F and genotyping with sequence-specific primers

Published online by Cambridge University Press:  01 May 1998

EVA-MARIA PRINZENBERG ,
PATRICIA ANGLADE ,
BRUNO RIBADEAU-DUMAS  and
GEORG ERHARDT
EVA-MARIA PRINZENBERG
Affiliation:
Institut fur Tierzucht und Haustiergenetik, Justus-Liebig-Universität Gießen, Ludwigstraße 21B, D-35390 Gießen, Deutschland
PATRICIA ANGLADE
Affiliation:
Unité de Recherche sur Biochimie et Structure des Protéines, INRA, F-78352 Jouy-en-Josas Cedex, France
BRUNO RIBADEAU-DUMAS
Affiliation:
Unité de Recherche sur Biochimie et Structure des Protéines, INRA, F-78352 Jouy-en-Josas Cedex, France
GEORG ERHARDT
Affiliation:
Institut fur Tierzucht und Haustiergenetik, Justus-Liebig-Universität Gießen, Ludwigstraße 21B, D-35390 Gießen, Deutschland
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Abstract

Bovine αs1-casein F (αs1-CN F) was found in a genetic resource of Deutsches Schwarzbuntes Niederungsrind cows at a frequency of 0·009. Biochemical characterization of this new variant was obtained by automated sequencing of reversed-phase HPLC-separated tryptic peptides of αs1-CN F and αs1-CN B. αs1-CN F was found to be a subtype of αs1-CN B with a single amino acid substitution (SerP/Leu) in position 66. DNA sequencing revealed a C/T transition in position 8418 of the gene. Sequence-specific primers were designed to perform an allele-specific polymerase chain reaction for detection of αs1CnF. Typing of artificial insemination sperm samples included in the genetic resource sperm pool identified one sire heterozygous for αs1CnF.

Type
Research Article
Information
Journal of Dairy Research , Volume 65 , Issue 2 , May 1998 , pp. 223 - 231
Copyright
Proprietors of Journal of Dairy Research 1998

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