Skip to main content Accessibility help

Association of MAP4K4 gene single nucleotide polymorphism with mastitis and milk traits in Chinese Holstein cattle

  • Dinesh Bhattarai (a1), Xing Chen (a1), Zia ur Rehman (a1), Xingjie Hao (a1), Farman Ullah (a1), Rahim Dad (a1), Hira Sajjad Talpur (a1), Ishwari Kadariya (a1), Lu Cui (a1), Mingxia Fan (a2) and Shujun Zhang (a1)...


The objective of the studies presented in this Research Communication was to investigate the association of single nucleotide polymorphisms present in the MAP4K4 gene with different milk traits in dairy cows. Based on previous QTL fine mapping results on bovine chromosome 11, the MAP4K4 gene was selected as a candidate gene to evaluate its effect on somatic cell count and milk traits in ChineseHolstein cows. Milk production traits including milk yield, fat percentage, and protein percentage of each cow were collected using 305 d lactation records. Association between MAP4K4 genotype and different traits and Somatic Cell Score (SCS) was performed using General Linear Regression Model of R. Two SNPs at exon 18 (c.2061T > G and c.2196T > C) with genotype TT in both SNPs were found significantly higher for somatic SCS. We found the significant effect of exon 18 (c.2061T > G) on protein percentage, milk yield and SCS. We identified SNPs at different location of MAP4K4 gene of the cattle and several of them were significantly associated with the somatic cell score and other different milk traits. Thus, MAP4K4 gene could be a useful candidate gene for selection of dairy cattle against mastitis and the identified polymorphisms might potentially be strong genetic markers.


Corresponding author

* For Correspondence; e-mail:


Hide All
Bouzakri, K, Ribaux, P & Halban, PA 2009 Silencing mitogen-activated protein 4 kinase 4 (MAP4K4) protects beta cells from tumor necrosis factor-alpha-induced decrease of IRS-2 and inhibition of glucose-stimulated insulin secretion. Journal of Biological Chemistry 284 2789227898
Chen, R, Yang, Z, Ji, D, Mao, Y, Chen, Y, Li, Y, Wu, H, Wang, X & Chang, L 2011 Polymorphisms of the IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein. Molecular Biology Reports 38 40834088
Delpire, E 2009 The mammalian family of sterile 20p-like protein kinases. Pflugers Archives 458 953967
Dror, N, Alter-Koltunoff, M, Azriel, A, Amariglio, N, Jacob-Hirsch, J, Zeligson, S, Morgenstern, A, Tamura, T, Hauser, H, Rechavi, G, Ozato, K & Levi, BZ 2007 Identification of IRF-8 and IRF-1 target genes in activated macrophages. Molecular Immunology 44 338346
Karin, M & Greten, FR 2005 NF-kappaB: linking inflammation and immunity to cancer development and progression. Nature Reviews Immunology 5 749759
Rupp, R & Boichard, D 1999 Genetic parameters for clinical mastitis, somatic cell score, production, udder type traits, and milking ease in first lactation Holsteins. Journal of Dairy Science 82 21982204
Schulman, NF, Sahana, G, Iso-Touru, T, Lund, MS, Andersson-Eklund, L, Viitala, SM, Varv, S, Viinalass, H & Vilkki, JH 2009 Fine mapping of quantitative trait loci for mastitis resistance on bovine chromosome 11. Animal Genetics 40 509515
Shook, GE & Schutz, MM 1994 Selection on somatic cell score to improve resistance to mastitis in the United States. Journal of Dairy Science 77 648658
Wang, C, Liu, M, Li, Q, Ju, Z, Huang, J, Li, J, Wang, H & Zhong, J 2011 Three novel single-nucleotide polymorphisms of MBL1 gene in Chinese native cattle and their associations with milk performance traits. Veterinary Immunology and Immunopathology 139 229236
Zohn, IE, Li, Y, Skolnik, EY, Anderson, KV, Han, J & Niswander, L 2006 p38 and a p38-interacting protein are critical for downregulation of E-cadherin during mouse gastrulation. Cell 125 957969


Type Description Title
Supplementary materials

Bhattarai supplementary material
Tables S1-S4

 PDF (565 KB)
565 KB


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed