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Antimicrobial activity of polypyrrole nanoparticles and aqueous extract of Moringa oleifera against Staphylococcus spp. carriers of multi-drug efflux system genes isolated from dairy farms

Published online by Cambridge University Press:  22 September 2020

José Givanildo da Silva ,
Mariana de Barros ,
Nataly Diniz de Lima Santos ,
Patrícia Maria Guedes Paiva ,
Thiago Henrique Napoleão ,
Maria José de Sena ,
Mateus Matiuzzi da Costa ,
Helinando Pequeno de Oliveira ,
Maria Aparecida Scatamburlo Moreira  and
Rinaldo Aparecido Mota
José Givanildo da Silva*
Affiliation:
Laboratório de Doenças Infectocontagiosas dos Animais Domésticos, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CP 52171-900, Recife, Pernambuco, Brazil
Mariana de Barros
Affiliation:
Laboratório de Doenças Bacterianas, Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Centro, CP 36570-900, Viçosa, MG, Brazil
Nataly Diniz de Lima Santos
Affiliation:
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, 1235, Cidade Universitária, CP 50670-901, Recife, Pernambuco, Brazil
Patrícia Maria Guedes Paiva
Affiliation:
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, 1235, Cidade Universitária, CP 50670-901, Recife, Pernambuco, Brazil
Thiago Henrique Napoleão
Affiliation:
Laboratório de Bioquímica de Proteínas, Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Professor Moraes Rego, 1235, Cidade Universitária, CP 50670-901, Recife, Pernambuco, Brazil
Maria José de Sena
Affiliation:
Laboratório de Doenças Infectocontagiosas dos Animais Domésticos, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CP 52171-900, Recife, Pernambuco, Brazil
Mateus Matiuzzi da Costa
Affiliation:
Laboratório de Microbiologia e Imunologia Animal, Universidade Federal do Vale do São Francisco, Rodovia BR-407 Km 12, Lote 543, C1, CP 56300-000, Petrolina, Pernambuco, Brazil
Helinando Pequeno de Oliveira
Affiliation:
Laboratório de Microbiologia e Imunologia Animal, Universidade Federal do Vale do São Francisco, Rodovia BR-407 Km 12, Lote 543, C1, CP 56300-000, Petrolina, Pernambuco, Brazil
Maria Aparecida Scatamburlo Moreira
Affiliation:
Laboratório de Doenças Bacterianas, Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Centro, CP 36570-900, Viçosa, MG, Brazil
Rinaldo Aparecido Mota
Affiliation:
Laboratório de Doenças Infectocontagiosas dos Animais Domésticos, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, CP 52171-900, Recife, Pernambuco, Brazil
*
Author for correspondence: José Givanildo da Silva, Email: givanildojgs@gmail.com
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Abstract

Our objectives were to identify genes of the multi-drug efflux system and to evaluate the antimicrobial activities of polypyrrole nanoparticles (PPy-NPs) and aqueous extract of Moringa oleifera against Staphylococcus spp. isolated from dairy farms in Northeast Brazil. Initially, 162 Staphylococcus spp. isolates were subjected to in vitro antimicrobial sensitivity tests. Of these, 35 presented antimicrobial multi-drug resistance phenotypes. These 35 isolates were then referred for the detection of norA, norB, norC, msrA, mgrA, tet-38, and lmrS genes, all of which feature in multi-drug efflux systems. In the isolates carrying the genes, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of PPy-NPs and Moringa oleifera aqueous extract were determined. In the molecular analysis of the 35 isolates norA, norC, tet-38, and msrA genes were detected and for the other genes norB, lmrS and mgrA there was no amplification. Antimicrobial activity was verified of PPy-NPs and aqueous extract of Moringa oleifera in Staphylococcus spp. carrying multi-drug efflux system genes. We concluded that there are multi-drug efflux system genes present in the Staphylococcus spp. from the agricultural environment in Northeast Brazil, and that aqueous extract of Moringa oleifera and PPy-NPs show bactericidal activity against these isolates.

Keywords

Agricultural environmentalternative therapiesantimicrobial resistancemastitis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 3 , August 2020 , pp. 309 - 314
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.

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