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An inhibitor typing scheme for Streptococcus uberis

Published online by Cambridge University Press:  01 June 2009

John R. Tagg
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand
Leone G. Vugler
Affiliation:
Department of Microbiology, University of Otago, Dunedin, New Zealand

Summary

A typing scheme was used to test 15 strains of Streptococcus uberis according to their production of (P-type) and sensitivity to (S-type) bacteriocin-like inhibitory substances. Twelve of the strains were inhibitor producers and nine different P-types were detected. All of the strains were typable according to inhibitor sensitivity, ten different S-types being distinguished. Both the P-type and S-type designations of the strains were reproducible on repeated testing. By combination of P-typing and S-typing, highly discriminatory inhibitor ‘fingerprints’ of the strains could be obtained. This scheme would appear to have considerable potential for typing isolates of Str. uberis as an aid to investigations into the epidemiology of Str. uberis mastitis in dairy cattle.

Type
Original Articles
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

REFERENCES

Bramley, A. J. 1984 Streptococcus uberis udder infection – a major barrier to reducing mastitis incidence. British Veterinary Journal 140 328335CrossRefGoogle Scholar
Bramley, A. J. & Dodd, F. H. 1984 Reviews of the progress of dairy science: mastitis control – progress and prospects. Journal of Dairy Research 51 481512CrossRefGoogle ScholarPubMed
Cullen, G. A. 1969 Streptococcus uberis: a review. Veterinary Bulletin 39 155165Google Scholar
Garvie, E. I. & Bramley, A. J. 1979 Streptococcus uberis: an approach to its classification. Journal of Applied Bacteriology 46 295304CrossRefGoogle Scholar
Jones, K. F. & Norcross, N. L. 1983 Immunochemical detection of a common antigen among Streptococcus uberis isolates. Journal of Clinical Microbiology 17 892897CrossRefGoogle ScholarPubMed
McDonald, T. J. & McDonald, J. S. 1976 Streptococci isolated from bovine intramammary infections. American Journal of Veterinary Research 37 377381Google ScholarPubMed
Schofield, C. R. & Tagg, J. R. 1983 Bacteriocin-like activity of group B and group C streptococci of human and of animal origin. Journal of Hygiene 90 718CrossRefGoogle Scholar
Tagg, J. R. 1984 Production of bacteriocin-like inhibitors by group A streptococci of nephritogenic M types. Journal of Clinical Microbiology 19 884887CrossRefGoogle Scholar
Tagg, J. R. & Bannister, L. V. 1979 ‘Fingerprinting’ β-haemolytic streptococci by their production of and sensitivity to bacteriocine-like inhibitors. Journal of Medical Microbiology 12 397411CrossRefGoogle ScholarPubMed
Tagg, J. R., Dajani, A. S. & Wannamaker, L. W. 1976 Bacteriocins of gram-positive bacteria. Bacteriological Reviews 40 722756CrossRefGoogle ScholarPubMed
Tagg, J. R. & Martin, D. R. 1980 Bacteriocin ‘fingerprinting’ of group B streptococcus strains of bovine and human origin. Proceedings of the University of Otago Medical School 58 2223Google Scholar
Ziv, G., Mushin, R. & Tagg, J. R. 1971 Pyocine typing as an epidemiological marker in Pseudomonas aeruginosa mastitis in cattle. Journal of Hygiene 69 171177CrossRefGoogle ScholarPubMed