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An improved method for the quantitative fractionation of casein mixtures using ion-exchange chromatography

Published online by Cambridge University Press:  01 June 2009

D. T. Davies  and
A. J. R. Law
D. T. Davies
Affiliation:
The Hannah Research Institute, Ayr, Scotland, KA6 5HL
A. J. R. Law
Affiliation:
The Hannah Research Institute, Ayr, Scotland, KA6 5HL
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Summary

A method for the quantitative analysis of casein mixtures is described. The method uses ion-exchange chromatography at 4 °C on DEAE cellulose, Whatman DE52 in the presence of tris-chloride-urea buffer (pH 8·6), and a NaCl gradient to fractionate the alkylated casein mixtures and a micro-biuret technique to determine protein concentrations. Values for fractions representative of (1) γ-caseins, (2) κ-casein plus some unknown proteins, (3) β-casein, (4) minor αs-caseins and (5) αs1- plus αs0-caseins were obtained, and whole casein from herd bulk milk was found to consist on average of about 2, 13, 36, 11 and 38% respectively of these fractions. Agreement between duplicate fractionations was satisfactory and the recovery of material varied between 94 and 98%.

Type
Original Articles
Information
Journal of Dairy Research , Volume 44 , Issue 2 , June 1977 , pp. 213 - 221
Copyright
Copyright © Proprietors of Journal of Dairy Research 1977

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