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Relationship between growth behaviour, micro and macroscopic morphologies and freezing sensitivity of the ripening starter Geotrichum candidum is strain specific and mostly related to the morphotypes: the arthrospores/hyphae parameter

Published online by Cambridge University Press:  10 September 2010

Ghalia Missous ,
Bouachanh Thammavongs ,
Géraldine Launay ,
Micheline Guéguen ,
Virginie Dieuleveux  and
Jean-Michel Panoff
Ghalia Missous
Affiliation:
Unité des Microorganismes d'Intérêts Laitier et Alimentaire, IBFA – IFR 146 ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France Laboratoire Départemental F. Duncombe, 1 rte Rosel, 14053 Saint Contest, France
Bouachanh Thammavongs
Affiliation:
Unité des Microorganismes d'Intérêts Laitier et Alimentaire, IBFA – IFR 146 ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France
Géraldine Launay
Affiliation:
Laboratoire Départemental F. Duncombe, 1 rte Rosel, 14053 Saint Contest, France
Micheline Guéguen
Affiliation:
Unité des Microorganismes d'Intérêts Laitier et Alimentaire, IBFA – IFR 146 ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France
Virginie Dieuleveux
Affiliation:
Laboratoire Départemental F. Duncombe, 1 rte Rosel, 14053 Saint Contest, France
Jean-Michel Panoff*
Affiliation:
Unité des Microorganismes d'Intérêts Laitier et Alimentaire, IBFA – IFR 146 ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France Plateau de cryobiologie – IFR 146 ICORE, Université de Caen Basse-Normandie, Esplanade de la Paix, 14032 Caen cedex, France.
*
*For correspondence; e-mail: jean-michel.panoff@unicaen.fr
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Abstract

Microscopic conformation, growth behaviour and freezing sensitivity of seven strains of Geotrichum candidum, a ripening starter, were studied and compared according to their macroscopic morphotypes. It has been shown that the thallus forming units (TFU)×ml−1/OD600nm ratio as a function of time is an interesting parameter to follow G. candidum sporulation through the growth behaviour. Microscopic conformation, growth behaviour and freezing sensitivity are clearly strain specific and mostly related to their corresponding morphotypes “yeast”, “mould” or “intermediate”. The two “mould” strains that sporulate weakly (UCMA103, UCMA499) showed a low survival rate to freezing stress whereas the “yeast” strains expressed a significant resistance owing to the arthrospore abundance. Interestingly, one strain (UCMA96) which appeared on solid medium in accord with the “mould” morphotype respond similarly to freezing stress.

Keywords

Geotrichum candidumStarterCheeseFungusMorphotypeCryopreservation
Type
Research Article
Information
Journal of Dairy Research , Volume 77 , Issue 4 , November 2010 , pp. 425 - 431
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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