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Oscillatory rheological study of the gelation mechanism of whey protein concentrate solutions: effects of physicochemical variables on gel formation

Published online by Cambridge University Press:  01 June 2009

Qingnong Tang ,
Owen J. McCarthy  and
Peter A. Munro
Qingnong Tang
Affiliation:
Department of Food Technology, Massey University, Palmerston North, New Zealand
Owen J. McCarthy
Affiliation:
Department of Food Technology, Massey University, Palmerston North, New Zealand
Peter A. Munro
Affiliation:
Department of Food Technology, Massey University, Palmerston North, New Zealand
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Summary

The thermal gelation of a commercially available whey protein concentrate was studied by oscillatory rheometry using a Bohlin rheometer. Gelation time increased with decreasing protein concentration with a critical protein concentration (at infinite gelation time) of 6·6%. The effect of temperature in the range 65–90 °C on gelation time was described by an Arrhenius equation with an activation energy of 154 kJ/mol. Gelation time was a minimum at pH 4–6, the isoelectric region of the whey proteins. Small additions of NaCl or CaCl2 dramatically decreased gelation time. Higher protein concentrations always produced higher storage modulus (G′) values after any heating time. Loss modulus (G″) v. time curves exhibited maxima at relatively short times for protein concentrations of 30 and 35%. G′ values for 10% protein concentration increased with temperature for heating times up to 59·5 min. G′ values at 59·5 min for 25% protein concentration were higher at 78 °C than at either 85 or 90 °C. The results are discussed in terms of current theories for biopolymer gelation.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 4 , November 1993 , pp. 543 - 555
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

REFERENCES

Beveridge, T., Jones, L. & Tung, M. A. 1984 Progel and gel formation and reversibility of gelation of whey, soybean, and albumen protein gels. Journal of Agricultural and Food Chemistry 32 307–313CrossRefGoogle Scholar
Bibbó, M. A. & Vallés, E. M. 1982 Calculation of average properties of the pendant chains in a network. Macromolecules 15 1293–1300CrossRefGoogle Scholar
Bibbó, M. A. & Vallés, E. M. 1984 Influence of pendant chains on the loss modulus of model networks. Macromolecules 17 360–365CrossRefGoogle Scholar
Bohlin, L., Hegg, P.-O. & Ljusberg-Wahren, H. 1984 Viscoelastic properties of coagulating milk. Journal of Dairy Science 67 729–734CrossRefGoogle Scholar
Clark, A. H. 1991 Structural and mechanical properties of biopolymer gels. In Food Polymers, Gels and Colloids, pp. 322–338 (Ed. Dickinson, E.). Cambridge: Royal Society of Chemistry (RSC Special Publication no. 82)CrossRefGoogle Scholar
Clark, A. H. & Ross-Murphy, S. B. 1987 Structural and mechanical properties of biopolymer gels. Advances in Polymer Science 83 57–192CrossRefGoogle Scholar
Doi, E. & Kitabatake, N. 1989 Structure of glycinin and ovalbumin gels. Food Hydrocolloids 3 327–337CrossRefGoogle Scholar
Doi, E., Koseki, T. & Kitabatake, N. 1987 Effects of limited proteolysis on functional properties of ovalbumin. Journal of the American Oil Chemists' Society 64 1697–1703CrossRefGoogle Scholar
Egelandsdal, B., Fretheim, K. & Harbitz, O. 1986 Dynamic rheological measurements on heat-induced myosin gels: an evaluation of the method's suitability for the filamentous gels. Journal of the Science of Food and Agriculture 37 944–954CrossRefGoogle Scholar
Harwalkar, V. R. & Kalab, M. 1985 Microstructure of isoelectric precipitates from β–lactoglobulin solutions heated at various pH values. Milchwissenschaft 40 665–668Google Scholar
Hermansson, A.-M. 1988 Gel structure of food biopolymers. In Food Structure - Its Creation and Evaluation, pp. 25–40 (Eds Blanshard, J. M. V. and Mitchell, J. R.). London: ButterworthsGoogle Scholar
Kitabatake, N., Tani, Y. & Doi, E. 1989 Rheological properties of heat-induced ovalbumin gels prepared by two-step and one-step heating methods. Journal of Food Science 54 1632–1638CrossRefGoogle Scholar
Moritaka, H., Fukuba, H., Kumeno, K., Nakahama, N. & Nishinari, K. 1991 Effect of monovalent and divalent cations on the rheological properties of gellan gels. Food Hydrocolloids 4 495–507CrossRefGoogle Scholar
Mulvihill, D. M. & Kinsella, J. E. 1987 Gelation characteristies of whey proteins and β–lactoglobulin. Food Technology 41(9) 102, 104, 106, 108, 110–111Google Scholar
Paulsson, M., Dejmek, P. & Van Vliet, T. 1990 Rheological properties of heat-induced β–lactoglobulin gels. Journal of Dairy Science 73 45–53CrossRefGoogle Scholar
Paulsson, M., Hegg, P.-O. & Castberg, H. B. 1986 Heat-induced gelation of individual whey proteins. A dynamic rheological study. Journal of Food Science 51 87–90CrossRefGoogle Scholar
Richardson, R. K. & Ross-Murphy, S. B. 1981 Mechanical properties of globular protein gels: 1. Incipient gelation behaviour. International Journal of Biological Macromolecules 3 315–322CrossRefGoogle Scholar
Robinson, B. P., Short, J. L. & Marshall, K. R. 1976 Traditional lactalbusmin – manufacture, properties and uses. New Zealand Journal of Dairy Science and Technology 11 114–126Google Scholar
Stading, M. & Hermansson, A.-M. 1990 Viscoelastic behaviour of β–lactoglobulin gel structures. Food Hydrocolloids 4 121–135CrossRefGoogle Scholar
Steventon, A. J., Gladden, L. F. & Fryer, P. J. 1991 A percolation analysis of the concentration dependence of the gelation of whey protein concentrates. Journal of Texture Studies 22 201–218CrossRefGoogle Scholar
Tung, C.-Y. M. & Dynes, P. J. 1982 Relationship between Viscoelastic properties and gelation in thermosestting systems. Journal of Applied Polymer Science 27 569–574CrossRefGoogle Scholar
Ziegler, G. R. & Foegeding, E. A. 1990 The gelation of proteins. Advances in Food and Nutrition Research 34 203–298CrossRefGoogle Scholar