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Effect of simulated gastrointestinal digestion on the antihypertensive properties of synthetic β-lactoglobulin peptide sequences

Published online by Cambridge University Press:  30 April 2007

Blanca Hernández-Ledesma
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). Madrid. Spain
Marta Miguel
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). Madrid. Spain
Lourdes Amigo
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). Madrid. Spain
Maria Amaya Aleixandre
Affiliation:
Instituto de Farmacología y Toxicología (CSIC). Departamento de Farmacología, Facultad de Medicina, Univ. Complutense, Madrid, Spain
Isidra Recio
Affiliation:
Instituto de Fermentaciones Industriales (CSIC). Madrid. Spain
Corresponding
E-mail address:

Abstract

In this study, the antihypertensive activity in spontaneously hypertensive rats of two peptides isolated from β-lactoglobulin hydrolysates with thermolysin was evaluated. These peptides, with sequences LLF [β-lg f(103–105)] and LQKW [β-lg f(58–61)], showed potent in vitro ACE-inhibitory activity. Two hours after administration, both sequences caused a clear and significant decrease in the blood pressure of these rats. The impact of a simulated gastrointestinal digestion on ACE-inhibitory and antihypertensive activities of these peptides was also studied. The results showed that both fragments were susceptible to proteolytic degradation after incubation with pepsin and Corolase PP®. In addition, their in vitro ACE-inhibitory activity decreased after the simulated digestion. It is likely that fragment LQK was the active end product of the gastrointestinal digestion of peptide LQKW. The fragment LL, observed after digestion of peptide LLF, probably exert its antihypertensive effect through a mechanism of action different than ACE-inhibition.

Type
Research Article
Copyright
Copyright © Proprietors of Journal of Dairy Research 2007

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