Hostname: page-component-77c89778f8-9q27g Total loading time: 0 Render date: 2024-07-17T10:40:05.689Z Has data issue: false hasContentIssue false
  • English
  • Français

Centrifugal fractionation of homogenized milks

Published online by Cambridge University Press:  01 June 2009

Douglas G. Dalgleish  and
Elizabeth W. Robson
Douglas G. Dalgleish
Affiliation:
Hannah Research Institute, Ayr, KA6 5HL, UK
Elizabeth W. Robson
Affiliation:
Hannah Research Institute, Ayr, KA6 5HL, UK
Get access Rights & Permissions [Opens in a new window]

Summary

A set of 12 fractions was isolated from homogenized cows' milk by a method of successive centrifugation steps. The fractions were analysed in terms of fat and protein contents and the diameters of the particles in them were also measured. Detailed comparisons of the turbidities of the samples and their light scattering properties allows comparison between them. It is concluded that the separation is less effective than a similar technique applied to casein micelles, but nonetheless that the different fractions do contain particles with different properties.

Type
Original Articles
Information
Journal of Dairy Research , Volume 52 , Issue 4 , November 1985 , pp. 539 - 546
Copyright
Copyright © Proprietors of Journal of Dairy Research 1985

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Chu, B. 1974 Laser Light Scattering. New York: Academic Press.Google Scholar
Dalgleish, D. G., Brinkhuis, J. & Payens, T. A. J. 1981 The coagulation of differently sized casein micelles by rennet. European Journal of Biochemistry 119 257–261CrossRefGoogle ScholarPubMed
Dickinson, E., Roberts, T., Robson, E. W. & Stainsby, G. 1984 Effect of salt on stability of casein stabilised butter oil-in-water emulsions. Lebensmittel-Wissenschaft und Technologie 17 107–110Google Scholar
Donnelly, W. J., Mcneill, G. P., Buchheim, W. & Mcgann, T. C. A. 1984 A comprehensive study of the relationship between size and protein composition in natural bovine casein micelles. Biochimica el Biophysica Acta 789 136–143CrossRefGoogle ScholarPubMed
Green, M. L., Marshall, R. J. & Glover, F. A. 1983 Influence of homogenization of concentrated milks on the structure and properties of rennet curds. Journal of Dairy Research 50 341–348CrossRefGoogle Scholar
Griffin, M. C. A. & Anderson, M. 1983 The determination of casein micelle size distribution in skim milk by chromatography and photon correlation spectroscopy. Biochimica et Biophysica Acta 748 453–459CrossRefGoogle Scholar
Horne, D. S. 1984 Determination of the size distribution of bovine casein micelles using photon correlation spectroscopy. Journal of Colloid and Interface Science 98 537–548CrossRefGoogle Scholar
Horne, D. S. & Dalgleish, D. G. 1985 A photon correlation spectroscopy study of size distributions of casein micelle suspensions. European Biophysics Journal 11 249–258CrossRefGoogle Scholar
Kerker, M. 1969 The scattering of light and other electromagnetic radiation. New York: Academic PressGoogle Scholar
Koppel, D. E. 1972 Analysis of macromolecular polydispersity in intensity correlation spectroscopy: the method of cumulants. Journal of Chemical Physics 57 4814–4820CrossRefGoogle Scholar
Oortwijn, H. & Walstra, P. 1979 The membranes of recombined fat globules. 2. Composition. Netherlands Milk and Dairy Journal 33 134–154Google Scholar
Oortwijn, H., Walstra, P. & Mulder, H. 1977 The membranes of recombined fat globules. 1. Electron microscopy. Netherlands Milk and Dairy Journal 31 134–147Google Scholar
Phillips, M. C. 1977 The conformation and properties of proteins at liquid interfaces. Chemistry and Industry 170–176Google Scholar
Robson, E. W. & Dalgleish, D. G. 1984 Coagulation of homogenized milk particles by rennet. Journal of Dairy Research 51 417–424CrossRefGoogle Scholar
Walstra, P. 1968 Estimating globule-size distribution of oil-in-water emulsions by spectroturbidimetry. Journal of Colloid and Interface Science 27 493–500CrossRefGoogle Scholar
Walstra, P. & Oortwijn, H. 1982 The membranes of recombined fat globules. 3. Mode of formation. Netherlands Milk & Dairy Journal 36 103–113Google Scholar