Hostname: page-component-76fb5796d-dfsvx Total loading time: 0 Render date: 2024-04-26T14:08:12.544Z Has data issue: false hasContentIssue false
  • English
  • Français

707. Phospholipids in New Zealand dairy products: I. The estimation of the total phospholipids and of lecithin, cephalin and sphingomyelin in butter

Published online by Cambridge University Press:  01 June 2009

A. K. R. McDowell
A. K. R. McDowell
Affiliation:
The Dairy Research Institute (N.Z.), Palmerston North, New Zealand
Get access Rights & Permissions [Opens in a new window]

Extract

A method has been described for the routine estimation of total phospholipids and of lecithin, cephalin and sphingomyelin in butter. Butter serum was treated with ether and light petroleum in a modified Röse-Gottlieb procedure to extract the phospholipids which then were estimated in the extract.

Chloroform-methanol extracted from the butter serum slightly more phosphorus than did the ether-light petroleum mixture. Ethanol-ether, benzene-ethanol and benzene-isopropanol, when used to extract a protein-phospholipid precipitate formed by the addition of trichloroacetic acid to butter serum, also removed slightly more phosphorus than did the ether-light petroleum mixture. The higher phosphorus contents of these extracts was due partly to a slightly more efficient extraction of phospholipids and partly to extraction of non-phospholipid phosphorus compounds.

Type
Original Articles
Information
Journal of Dairy Research , Volume 25 , Issue 2 , June 1958 , pp. 192 - 201
Copyright
Copyright © Proprietors of Journal of Dairy Research 1958

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

(1)Holm, G. E., Wright, P. A. & Deysher, E. F. (1936). J. Dairy Sci. 19, 631.Google Scholar
(2)Wittcoff, H. (1951). The Phosphatides, 1st ed. p. 322. New York: Reinhold Publishing Corporation.Google Scholar
(3)Koch, W. & Woods, H. S. (1905–1906). J. biol. Chem. 1, 203.CrossRefGoogle Scholar
(4)Brodrick-Pittard, N. A. (1914). Biochem. Z. 67, 382. Abstract in Chem. Abstr. (1915), 9, 639.Google Scholar
(5)Osborne, T. B. & Wakeman, A. J. (1915). J. biol. Chem. 21, 539.CrossRefGoogle Scholar
(6)Palmer, L. S. & Wiese, H. F. (1933). J. Dairy. Sci. 16, 41.CrossRefGoogle Scholar
(7)Kurtz, F. E., Jamieson, G. S. & Holm, G. E. (1934). J. biol. Chem. 106, 717.CrossRefGoogle Scholar
(8)Crane, J. C. & Horrall, B. E. (1943). J. Dairy Sci. 26, 935.CrossRefGoogle Scholar
(9)Baliga, B. S. & Basu, K. P. (1956). Indian J. Dairy Sci. 9, 24.Google Scholar
(10)Baliga, B. S. & Basu, K. P. (1956). Indian J. Dairy Sci. 9, 95.Google Scholar
(11)Osborne, T. B. & Wakeman, A. J. (1915). J. biol. Chem. 21, 91.CrossRefGoogle Scholar
(12)Haylock, O. F. (1948). Dairy Research Institute (N.Z.). Private communication.Google Scholar
(13)Pont, E. G. (1952). J. Dairy Res. 19 316.CrossRefGoogle Scholar
(14)Jack, E. L. & Smith, L. M. (1956). J. Dairy Sci. 39, 1.Google Scholar
(15)Hess, A. F. & Helman, F. O. (1925). J. biol. Chem. 64, 781.Google Scholar
(16)Holm, G. E., Wright, P. A. & Deysher, E. F. (1933). J. Dairy sci. 16, 445.Google Scholar
(17)Hack, M. H. (1947). J. biol. Chem. 169, 137.CrossRefGoogle Scholar
(18)Grossfeld, J. & Zeisset, A. (1943). Z. Untersuch. Lebensmitt. 85, 321. Abstract in Dairy Sci. Abstr. (1944), 5, 205.CrossRefGoogle Scholar
(19)Mohr, W. (1950). Milchwissenschaft. 5, 121.Google Scholar
(20)Kieferle, F., Feichtner, C. & Pirner, G. (1950). Arb. Ber. Suddtsch. Vers.-u. Forsch. Milchw. Weihenstephan, 1, 37. Abstract in Dairy Sci. Abstr. (1952), 14, 377.Google Scholar
(21)Van slyke, D. D. & Sacks, J. (1953). J. biol. Chem. 200, 525.CrossRefGoogle Scholar
(22)Folch, J., Ascoli, I., Lees, M., Meath, J. A. & Le Baron, F. N. (1951). J. biol. Chem. 191, 833.Google Scholar
(23)Sperry, W. M. & Brand, F. C. (1955). J. biol. Chem. 213, 69.CrossRefGoogle Scholar
(24)Lea, C. H. & Wilson, R. A. L. (1955). J. Sci. Fd Agric. 6, 153.Google Scholar
(25)Stewart, C. P. & Hendry, E. B. (1935) Biochem. J. 29, 1683.Google Scholar
(26)Baumann, E. J. (1924). J. biol. Chem. 59, 667.CrossRefGoogle Scholar
(27)King, E. J. (1932). Biochem. J. 26, 292.CrossRefGoogle Scholar
(28)Griswold, B. L., Humoller, F. L. & McIntyre, A. R. (1951). Anal. Chem. 23, 192.CrossRefGoogle Scholar
(29)Axelrod, J., Reichenthal, J. & Brodie, B. B. (1953). J. biol. Chem. 204, 903.Google Scholar
(30)Weil-Malherbe, H. (1953). Biochem. J. 55, 745.Google Scholar
(31)Fiske, C. H. & Subbarow, Y. (1925). J. biol. Chem. 66, 375.CrossRefGoogle Scholar
(32)Russell, J. A. (1944). J. biol. Chem. 156, 457.CrossRefGoogle Scholar
(33)Johnson, A. C., McNabb, A. R. & Rossiter, R. J. (1948). Biochem. J. 43, 573.Google Scholar
(34)Glick, I. (1944). J. biol. Chem. 156, 643.CrossRefGoogle Scholar
(35)Sperry, W. M. & Brand, F. C. (1925). Fed. Proc. 4, 104.Google Scholar
(36)Winzler, R. J. & Meserve, E. R. (1945). J. biol. Chem. 159, 395.Google Scholar
(37)sperry, W. M. & Brand, F. C. (1948). Fed. Proc. 7, 191.Google Scholar
(38)shorland, F. B. (1946). Fats Research Laboratory, D.S.I.R. Wellington, N.Z. Private communication.Google Scholar
(39)Baliga, B. S. & Basu, K. P. (1955). Indian J. Dairy Sci. 8, 119.Google Scholar
(40)McDowell, A. K. R. (1958). J. Dairy Res. 25, 202.Google Scholar
(41)Horrall, B. E. (1935). Bull. Ind. agric. Exp. Sta. no. 401.Google Scholar
(42)Perlman, J. L. (1935). J. Dairy Sci. 18, 113.CrossRefGoogle Scholar
(43)Wiese, H. F., Nair, J. H. & Fleming, R. S. (1932). Industr. Engng Chem. (Anal.), 4, 362.CrossRefGoogle Scholar
(44)Kenyon, J. & Jenness, R. (1956). J. Dairy Sci. 39, 909.Google Scholar
(45)Linderstrom-Lang, K. (1929). C. R. Lab. Carlsberg, 17, no. 9.Google Scholar