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Validation of an Analytical Method for the Determination of the Activity of Protease in Animal Feed Additives and in Animal Feedingstuffs

Published online by Cambridge University Press:  23 January 2015

G.P. Dillon ,
K. Filer  and
C.A. Moran
G.P. Dillon*
Affiliation:
Alltech Ireland, Sarney, Summerhill Road, Dunboyne, Co Meath, Ireland
K. Filer
Affiliation:
Alltech Inc, 3031 Catnip Hill Pike, Nicholasville, 40356 KY, USA
C.A. Moran
Affiliation:
Alltech France SARL, ZI Belle Etoile, 25 allée des Sapins, 44483 Carquefou, France
*
*Corresponding author:gdillon@alltech.com
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Summary

The nutrient availability in animal feeds can be improved by including exogenous enzymes to the feed, either by helping breakdown anti-nutritional factors or by increasing digestibility of complex ingredients thereby releasing more nutrients for utilisation. This process can improve the efficiency of meat and egg production, increase animal health, decrease feeding costs and reduce nutrients in animal waste. Proteases are protein-digesting enzymes that are used in animal nutrition to break down storage proteins in various plant materials and proteinaceous anti-nutrients in vegetable proteins. The analysis of exogenous proteases in feed additives and after they have been added to feed has proven technically challenging. Accordingly, the purpose of this work was to validate a method for the determination of the activity of protease in animal feed additives and supplemented animal feed. The approach used for the assay was to adapt an assay based on the hydrolysis of haemoglobin. The method validations examined a range of parameters including; linearity & range; uncertainty, sensitivity, accuracy and studies designed to highlight any possible matrix effects on various types of supplemented feed. The assay method described herein is convenient and inexpensive and could be applied to the analysis of proteases in animal feeds during quality control and in investigating fraudulent adulteration of feed to ensure the authenticity and traceability of the product.

Keywords

Protease method validationenzymeanimal feed
Type
Original Research
Information
Journal of Applied Animal Nutrition , Volume 3 , 2015 , e1
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2015 

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