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A natural carbohydrate fraction Actigen™ from Saccharomyces cerevisiae cell wall: effects on goblet cells, gut morphology and performance of broiler chickens

Published online by Cambridge University Press:  16 August 2013

H. Lea ,
P. Spring ,
J. Taylor-Pickard  and
E. Burton
H. Lea*
Affiliation:
School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Nottingham, UK
P. Spring
Affiliation:
Swiss College of Agriculture, Switzerland
J. Taylor-Pickard
Affiliation:
Alltech Ireland, Dunboyne, Ireland
E. Burton
Affiliation:
School of Animal, Rural and Environmental Sciences, Nottingham Trent University, Nottingham, UK
*
*Corresponding author: harriet.lea@ntu.ac.uk
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Summary

A study was conducted to evaluate a natural carbohydrate fraction Actigen™ (NCF), derived from mannanoligosaccharide, in feed on growth performance, intestinal morphology and goblet cell number and area of male broilers'. Dietary treatments included: 1) control diet (antibiotic and NCF free), 2) NCF at 200 g/t, 3) NCF at 400 g/t, and 4) NCF 800 g/t. Two hundred and forty birds were placed into 12 replicate pens per treatment (5 birds/pen), sixty birds per treatment. Body weight and feed intake were recorded weekly up to day 42. At this time a 2.5cm section of jejunum and duodenum were excised post mortem for morphological analysis. Birds fed 200 g/t and 800 g/t NCF were significantly (P < 0.01) heavier from day 14 onwards than the control birds. Feed intake was significantly higher in birds fed 200 g/t NCF compared to those fed the control at 21 and 35 days (P < 0.05). Diets containing 200 g/t and 800 g/t of NCF significantly decreased broiler feed conversion ratio (FCR) compared to the control in the first phase (1–14 days) (P < 0.01) and levels of NCF decreased FCR (P < 0.05) in the second phase (15–28 days). NCF had no significant effect on villus height, villus width, crypt depth or villus to crypt ratio in either duodenum or jejunum. NCF did not significantly affect goblet cell area or goblet cell number in the duodenum, however, in the jejunum, 800 g/t NCF significantly (P < 0.05) increased goblet cell area over the control. In conclusion, NCF showed a positive effect on broiler performance in the starter and grower phases, and increased goblet cell area in the jejunum, suggesting higher levels of mucin production. This indicated that the performance benefit of NCF could be age-dependent, with younger birds responding more than the older ones. There were no additional benefits to performance when feeding NCF for a longer period (after 28 d of age), however it is postulated that birds fed NCF would have greater defence to pathogenic challenge through increased storage capacity of mucin.

Keywords

Actigen™prebioticvillibroilergoblet cell
Type
Original Research
Information
Journal of Applied Animal Nutrition , Volume 1 , 2012 , e9
Copyright
Copyright © Cambridge University Press and Journal of Applied Animal Nutrition Ltd. 2013 

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References

Baurhoo, B., Phillip, L. & Ruiz-Feria, C. (2007) Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poultry science, 86: 1070–1078.CrossRefGoogle ScholarPubMed
Baurhoo, B., Ferket, P. & Zhao, X. (2009) Effects of diets containing different concentrations of mannanoligosaccharide or antibiotics on growth performance, intestinal development, cecal and litter microbial populations, and carcass parameters of broilers. Poultry Science, 88: 2262–2272.CrossRefGoogle ScholarPubMed
Blomberg, L., Krivan, H., Cohen, P. & Conway, P. (1993) Piglet ileal mucus contains protein and glycolipid (galactosylceramide) receptors specific for Escherichia coli k88 fimbriae. Infection and Immunity, 61: 2526–2531 CrossRefGoogle ScholarPubMed
Brümmer, M., Jansen van Rensburg, C. & Moran, C. (2010) Saccharomyces cerevisiae cell wall products: The effects on gut morphology and performance of broiler chickens. South African Journal of Animal Science, 40: 14–21.CrossRefGoogle Scholar
Castillo, M., Martın-Orue, S., Taylor-Pickard, J., Perez, J. & Gasa, J. (2008) Use of mannan-oligosaccharides and zinc chelate as growth promoters and diarrhea preventative in weaning pigs: Effects on microbiota and gut function. Journal of Animal Science, 86: 94–101.CrossRefGoogle Scholar
Che, T., Song, M., Liu, Y., Johnson, R., Kelley, K., Van Alstine, W., Dawson, K. & Pettigrew, J. (2012) Mannan oligosaccharide increases serum concentrations of antibodies and inflammatory mediators in weanling pigs experimentally infected with porcine reproductive and respiratory syndrome virus. Journal of Animal Science. 90: 2784–93.CrossRefGoogle ScholarPubMed
Chee, S. (2008) Functional interactions of mannooligosaccharides with dietary threonine on chicken gastrointestinal tract. Ph. D. Thesis, The University of New England.Google Scholar
Chee, S., Iji, P., Choct, M., Mikkelsen, L. & Kocher, A. (2010) Functional interactions of manno-oligosaccharides with dietary threonine in chicken gastrointestinal tract. I. growth performance and mucin dynamics. British Poultry Science. 51: 677–685.CrossRefGoogle ScholarPubMed
De Los Santos, F., Donoghue, A., Farnell, M., Huff, G., Huff, W. & Donoghue, D. (2007) Gastrointestinal maturation is accelerated in turkey poults supplemented with a mannan-oligosaccharide yeast extract (Alphamune). Poultry Science, 86: 921–930.CrossRefGoogle Scholar
Dibner, J. & Richards, D. (2005) Antibiotic growth promoters in agriculture: history and mode of action. Poultry Science, 84: 634–643.CrossRefGoogle ScholarPubMed
Freitas, M., Tavan, E., Cayuela, C., Diop, L., Sapin, C. & Trugnan, G. (2003) Hostpathogens cross-talk. Indigenous bacteria and probiotics also play the game. Biology of the Cell, 95: 503–506.CrossRefGoogle ScholarPubMed
Gao, J., Zhang, H., Yu, S., Wu, S., Yoon, I., Quigley, J., Gao, Y. & Qi, G. (2008) Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poultry Science, 87: 1377–1384.CrossRefGoogle ScholarPubMed
Huff, G., Huff, W., Rath, N. & Tellez, G. (2006) Limited treatment with [beta]-1,3/1,6-Glucan improves production values of broiler chickens challenged with escherichia coli. Poultry Science, 85: 613–618.CrossRefGoogle ScholarPubMed
Iji, P., Saki, A. & Tivey, D. (2001) Intestinal structure and function of broiler chickens on diets supplemented with a mannanoligosaccharide. Journal of the Science of Food and Agriculture, 81: 1186–1192.CrossRefGoogle Scholar
Jung, S., Houde, R., Baurhoo, B., Zhao, X. & Lee, B. (2008) Effects of galacto-oligosaccharides and a bifidobacteria lactis-based probiotic strain on the growth performance and fecal microflora of broiler chickens, Poultry Science, 87: pp1694–1699.CrossRefGoogle Scholar
Katayama, T., Fujita, K. & Yamamoto, K. (2005) Novel bifidobacterial glycosidases acting on sugar chains of mucin glycoproteins. Journal Of Bioscience And Bioengineering, 99: 457–465.CrossRefGoogle ScholarPubMed
Kocher, A., Canolly, A., Zawadzki, J. & Gallet, D. (2004) The challenge of finding alternatives to antibiotic growth promoters. International Society for Animal Hygiene-Saint Malo 2004, p.227–229.Google Scholar
Koutsos, E. & Arias, V. (2006) Intestinal ecology: interactions among the gastrointestinal tract, nutrition, and the microflora. Journal of Applied Poultry Research, 15: 161–173.CrossRefGoogle Scholar
Mack, D., Michail, S., Wei, S., McDougall, L. & Hollingworth, M. (1999) Probiotics inhibit enteropathogenic E. coli adherence in vitro by inducing intestinal mucin gene expression. American Journal of Physiology Gastrointestinal and Liver Physiology, 276: 941–950.CrossRefGoogle ScholarPubMed
Midilli, M., Alp, M., Kocabagh, N., Muglali, O.H., Turan, N., Yilmaz, H. & Cakir, S. (2008) Effects of dietary probiotic and prebiotic supplementation on growth performance and serum IgG concentations of a broiler. South African Journal of Animal Science, 38: 21–27.CrossRefGoogle Scholar
Miguel, J., Rodriguez-Zas, S. & Pettigrew, J. (2004) Efficacy of a mannan oligosaccharide (Bio-Mos) for improving nursery pig performance. Journal of Swine Health and Production, 12: pp 296–307.Google Scholar
Morales-lopez, R., Auclair, E., Van Immerseel, F., Ducatelle, R., Garcia, F. & Brufau, J. (2010) Effects of different yeast cell wall supplements added to maize- or wheat-based diets for broiler chickens. British Poultry Science, 51: 399–408.CrossRefGoogle ScholarPubMed
Mourao, J., Pinheiro, V., Alves, A., Guedes, C., Pinto, L., Saavedra, M., Spring, P. & Kocher, A. (2006) Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits. Animal Feed Science and Technology, 126: 107–120.CrossRefGoogle Scholar
Muthusamy, N., Halder, S., Ghosh, T. & Bedford, M. (2012) Effects of hydrolysed saccharomyces cerevisiae yeast and yeast cell wall components on live performance, intestinal histo-morphology and humoral immune response of broilers. British Poultry Science, 52: pp 694–703.CrossRefGoogle Scholar
Ofek, I., Mirelmann, D. & Sharon, N. (1977) Adherence of E. coli to human mucosal cells mediated by mannose receptors. Nature, 265: pp623–625.CrossRefGoogle ScholarPubMed
Ruas-Madiedo, P., Gueimonde, M., Fernandez-Garcia, M., Reyes-Gavilan, C. & Margolles, A. (2008) Mucin degradation by bifidobacterium strains isolated from the human intestinal microbiota. Applied And Environmental Microbiology, 74: 1936–1940.CrossRefGoogle ScholarPubMed
Reisinger, N., Ganner, A., Masching, S., Schatzmayr, G. & Applegate, T.J. (2012) Efficacy of a yeast derivative on broiler performance, intestinal morphology and blood profile. Livestock science, 143: pp195 –200.CrossRefGoogle Scholar
Santin, E., Maiorka, A. & Macari, A. (2001) Performance and intestinal mucosa development of broiler chickens fed diets containing Saccharomyces cerevisiae cell wall. Journal of Applied Poultry Research, 10: 236–244.CrossRefGoogle Scholar
Sims, M., Dawson, K., Newman, K., Spring, P. & Hooge, D. (2004) Effects of dietary mannan oligosaccharide, bacitracin methylene disalicylate, or both on the live performance and intestinal microbiology of turkeys. Poultry Science, 83: 1148–1154.CrossRefGoogle ScholarPubMed
Smirnov, A., Perez, R., Amit-Romach, E., Sklan, D. & Uni, Z. (2005) Mucin dynamics and microbial populations in the chicken small intestine are change by dietary probiotic and antibiotic growth promoter supplementation. Journal of Nutrition, 135: 187–192.CrossRefGoogle ScholarPubMed
Smirnov, A., Sklan, D. & Uni, Z. (2004) Mucin dynamics in the chick small intestine are altered by starvation. Journal of Nutrition, 134: 736–742.CrossRefGoogle ScholarPubMed
Smirnov, A., Tako, E., Ferket, P. & Uni, Z. (2006) Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydrates. Poultry Science, 85: pp669–673.CrossRefGoogle ScholarPubMed
Sohail, M., Hume, M., Byrd, J., Nisbet, D., Ijaz, A., Sohail, A., Shabbir, M. & Rehman, H. (2012) Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poultry Science, 91: pp2235–40.CrossRefGoogle ScholarPubMed
Spring, P., Wenk, C., Dawson, K. & Newman, K. (2000) The effects of dietary mannanoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of Salmonella-challenged broiler chicks. Poultry Science, 79: pp205–211.CrossRefGoogle ScholarPubMed
Sun, X., McElroy, A., Webb, K., Sefton, A. & Novak, C. (2005) Broiler performance and intestinal alterations when fed drug-free diets. Poultry Science, 84: 1294–1302.CrossRefGoogle ScholarPubMed
Thomke, S. & Elwinger, K. (1998) Growth promotants in feeding pigs and poultry I. Growth and feed efficiency responses to antibiotic growth promotants. Annales De Zootechnie, 47: 85–97.CrossRefGoogle Scholar
Uni, Z. & Smirnov, A. (2006) Modulating mucin dynamics using functional carbohydrates. Reproduction Nutrition Development, 46: S76.Google Scholar
White, L., Newman, M., Cromwell, G. & Lindemann, M. (2002) Brewers dried yeast as a source of mannan oligosaccharides for weanling pigs. Journal of Animal Scicence, 80:2619–2628.Google ScholarPubMed
Yason, C., Summers, B. & Schat, K. (1987) Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. American Journal of Veterinary Research, 48: 927–38.Google ScholarPubMed
Yitbarek, A., Echeverry, H., Brady, J., Hernandez-Doria, J., Camelo-Jaimes, G., Sharif, S., Guenter, W., House, J. & Rodriguez-Lecompe, J. (2012) Innate immune response to yeast- derived carbohysrates in broiler chickens fed organic diets and challenged with Clostridium perfringens. Poultry Science, 91: pp1105–1112.CrossRefGoogle ScholarPubMed
Zhang, A., Lee, B., Lee, S., Lee, K., An, G., Song, K. & Lee, C. (2005) Effects of yeast (Saccharomyces cerevisiae) cell components on growth performance, meat quality, and ileal mucosa development of broiler chicks. Poultry Science, 84: 1015–1021.CrossRefGoogle ScholarPubMed