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In vivo fibre optic confocal imaging of microvasculature and nerves in the rat vas deferens and colon

Published online by Cambridge University Press:  01 May 1998

G. D. PAPWORTH
Affiliation:
Department of Pharmacology, Monash University, Clayton
P. M. DELANEY
Affiliation:
Optiscan Imaging Ltd., Notting Hill, Victoria, Australia
L. J. BUSSAU
Affiliation:
Department of Pharmacology, Monash University, Clayton
L. T. VO
Affiliation:
Department of Pharmacology, Monash University, Clayton
R. G. KING
Affiliation:
Department of Pharmacology, Monash University, Clayton
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Abstract

A fluorescence confocal microscopy technique was employed to obtain subsurface images of nerve and microvascular structure in the vas deferens and colon of the living rat. The use of dual labelling with vital dyes and 2-channel confocal acquisition allowed differentiation of microscopic structure at both low and higher magnification. Characteristic staining patterns of nerves and blood vessels were repeatedly obtained in each tissue, suggesting the potential of this technique for studying morphological changes associated with surgical procedures and/or models of neuronal or vascular pathology.

Type
Research Article
Copyright
© Anatomical Society of Great Britain and Ireland 1998

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