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Variation in ovine KRTAP8-1 is associated with variation in wool fibre staple strength and curvature

Published online by Cambridge University Press:  21 October 2019

H. Gong ,
H. Zhou Open the ORCID record for H. Zhou [Opens in a new window] ,
W. Li ,
J. Wang Open the ORCID record for J. Wang [Opens in a new window] ,
S. Li ,
Y. Luo  and
J. G. H. Hickford
H. Gong
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
H. Zhou
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
W. Li
Affiliation:
Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou730070, China
J. Wang
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou730070, China
S. Li
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou730070, China
Y. Luo*
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gansu Key Laboratory of Herbivorous Animal Biotechnology, Faculty of Animal Science and Technology, Gansu Agricultural University, Lanzhou730070, China
J. G. H. Hickford*
Affiliation:
International Wool Research Institute, Gansu Agricultural University, Lanzhou730070, China Gene-Marker Laboratory, Faculty of Agriculture and Life Sciences, Lincoln University, Lincoln7647, New Zealand
*
Author for correspondence: Y. Luo, E-mail: luoyz@gsau.edu.cn and J. G. H. Hickford, E-mail: jon.hickford@lincoln.ac.nz
Author for correspondence: Y. Luo, E-mail: luoyz@gsau.edu.cn and J. G. H. Hickford, E-mail: jon.hickford@lincoln.ac.nz
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Abstract

KRTAP8-1 was the initial high-glycine-tyrosine keratin-associated protein gene recognized in sheep, but little is known about the functional influence of this gene. The current study used polymerase chain reaction-single stranded conformational polymorphism analysis to genotype KRTAP8-1 in 391 Southdown × Merino-cross sheep from six sire-lines. Five previously described variants (named A to E) of KRTAP8-1 were identified with frequencies of 67.0, 14.2, 7.0, 10.7 and 1.0%, respectively. Of the four variants (A, B, C and D) that occurred at a frequency greater than 5%, the presence of C was found to be associated with a reduction in mean fibre curvature (MFC) and the presence of D was associated with an increase in mean staple strength (MSS), whereas the presence of A had a trend of association with reduced MSS. Associations were not identified with other wool traits. These results suggest that variation in KRTAP8-1 affects MSS and MFC, and that KRTAP8-1 has the potential to be used as a genetic marker for improving these traits.

Keywords

Fibre curvaturefibre staple strengthkeratin-associated protein 8-1 gene (KRTAP8-1)sheepvariationwool
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 157 , Issue 6 , August 2019 , pp. 550 - 554
Copyright
Copyright © Cambridge University Press 2019

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