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Nutrition and growth of gnotobiotic lambs

Published online by Cambridge University Press:  27 March 2009

R. J. Lysons ,
T. J. L. Alexander  and
Patricia D. Wellstead
R. J. Lysons
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire.
T. J. L. Alexander
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire.
Patricia D. Wellstead
Affiliation:
Agricultural Research Council, Institute for Research on Animal Diseases, Compton, Newbury, Berkshire.
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Summary

Seventeen gnotobiotic lambs were reared up to 21 weeks of age on cows’ milk followed by sterile solid diets similar to diets fed to conventional lambs. Seven were given limited defined populations of rumen bacteria, seven were left uninoculated and three were dosed with rumen contents from conventional sheep (‘conventionalized’). The lambs were reared in four groups corresponding to four lambing seasons.

Gnotobiotic lambs fed cows’ milk ad libitumgrew at similar rates and converted feed into live-weight gain with similar efficiency to conventional lambs. The gnotobiotic lambs irrespective of inoculation and the two conventionalized lambs in the first three groups failed to maintain growth on solid diets when milk feeding had been stopped. However, three gnotobiotic lambs in the fourth group, two of which were inoculated, continued to grow satisfactorily for at least 6 weeks on a solid diet alone, and the inoculation of defined populations of bacteria appeared to confer an advantage. The conventionalized lamb in the fourth group suffered a severe temporary setback, following which it grew well.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 88 , Issue 3 , June 1977 , pp. 597 - 604
Copyright
Copyright © Cambridge University Press 1977

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References

Alexander, T. J. L. & Lysons, R. J. (1971). Observations on rearing gnotobiotic lambs. British Veterinary Journal 127, 349–57.CrossRefGoogle ScholarPubMed
Alexander, T. J. L., Lysons, R. J., Elliott, L. M. & Wellstead, P. D. (1973a). Equipment for rearing gnotobiotic lambs. Laboratory Animals 7, 195–217.CrossRefGoogle ScholarPubMed
Alexander, T. J. L., Lysons, R. J., Elliott, L. M. & Wellstead, P. D. (1973b). Techniques for realing gnotobiotic lambs. Laboratory Animals 7, 239–54.CrossRefGoogle Scholar
Elliott, L. M., Alexander, T. J. L., Wellstead, P. D. & Lysons, R. J. (1974). Microbiological monitoring of gnotobiotic lambs. Laboratory Animals 8, 51–9.CrossRefGoogle ScholarPubMed
KÜSter, E. (1915). Die gewinnung, haltung und aufzucht keimfreier tiere und [ihre bedeutung für dio erforschung naturlioher lebersvorgange. Arbeiten aus der K. Oesundheitsamte 48, 1–79.Google Scholar
Large, R. V. (1965). The effect of concentration of milk substitute on the performance of artificially reared lambs. Animal Production 7, 325–31.Google Scholar
Lysons, R. J. (1974). Studies of lambs with a limited microflora. Ph.D. thesis, University of Cambridge.Google Scholar
Lysons, R. J. (1975). The establishment of Bacteroides ruminicola in a gnotobiotic lamb. Journal of General Microbiology 87, 170–2.CrossRefGoogle Scholar
Lysons, R. J. & Alexander, T. J. L. (1975). The gnotobiotic ruminant and in vivo studies of defined bacterial populations. In Digestion and Metabolism in the Ruminant(ed. McDonald, I. W. and Warner, A. C. I.), pp. 180–92. Armidale: University of New England.Google Scholar
Lysons, R. J., Alexander, T. J. L., Wellstead, P. D., Hobson, P. N., Mann, S. O. & Stewart, C. S. (1976a). Defined bacterial populations in the rumens of gnotobiotic lambs. Journal of General Microbiology 94, 257–69.CrossRefGoogle ScholarPubMed
Lysons, R. J., Alexander, T. J. L., Wellstead, P. D. & Jennings, I. W. (1976b). Observations on the alimentary tract of gnotobiotic lambs. Research in Veterinary Science 20, 70–6.CrossRefGoogle ScholarPubMed
Morgan, J. A. & Owen, J. B. (1972). Nutrition of artificially reared lambs. 2. The effect of feed restriction at three stages of growth on growth and carcass composition. Animal Production 15, 293—300.Google Scholar
Orskov, E. R., Davies, P. J., Dickson, I. A., Humphries, A. B., Rutter, W. & Treacher, T. T. (1972). Protein utilization and performance of earlyweaned lambs. Animal Production 15, 183—7.Google Scholar
Oxender, W. D., Purser, D. B. & Bergen, W. G. (1971). A procedure to obtain germ-free goats by hysterotomy. American Journal of Veterinary Research 32, 1443–6.Google ScholarPubMed
Penning, P. D. (1967). Artificial rearing of lambs. Annual Report of the Grasslands Research Institute, Hurley 1966, pp. 86–92.Google Scholar
Ponto, K. H. & Bergen, W. G. (1974). Developmental aspects of glucose and V.F.A. metabolism in the germfree and conventional ruminant. Journal of Animal Science 38, 893–9.CrossRefGoogle Scholar
Purser, D. B. & Bergen, W. G. (1969). Glucose utilization and hepatic enzyme activities in young gnotobiotic ruminants. Journal of Dairy Science 52, 790–5.CrossRefGoogle ScholarPubMed
Smith, C. K. (1966). The derivation and characterization of the germfree ruminant. Ph.D. thesis, University of Notre Dame, Indiana.Google Scholar
Soares, J. H., Leffel, E. C. & Larsen, R. K. (1970). Neonatal lambs in a gnotobiotic environment. Journal of Animal Science 31, 733–40.CrossRefGoogle Scholar
Stewart, C. S. & Lysons, R. J. (1975). Fermentation products in the rumen of a gnotobiotic lamb dosed with Bacteroides ruminicola. Proceedings of the Nutrition Society 34, 72A.Google ScholarPubMed
Trexler, P. C. (1971). Microbiological isolation of large animals. Veterinary Record 88, 15–20.CrossRefGoogle ScholarPubMed
Weaver, J. M. (1974). The establishment of Ruminococcus flavefaciens in gnotobiotic lambs. Ph.D. thesis University of Maryland.Google Scholar