Further studies of the behaviour of cellulose-splitting bacteria in artificial media have been made in an attempt to account for the manner in which cellulose is utilized for fat production in the ruminant. The behaviour of thermophilic bacteria, with an optimum temperature of fermentation in the neighbourhood of 65°C, has been compared with that of the micro-organisms capable of fermenting at 37°C. In both cases the cultures were obtained from the rumen contents of sheep.

By the addition of toluene at the “head” stage of fermentation, it was possible to demonstrate, both at 37 and 65°C., the production of small amounts of glucose during subsequent incubation. Glucose is clearly a primary breakdown product in the bacterial fermentation of cellulose.

An investigation has been made of the nature of the volatile fatty acids produced by bacterial decomposition of shredded filter paper. No consistent differences from this standpoint were noted between the reactions as carried out at 37 and 65°C., but the nature and proportions of the acids varied considerably from culture to culture. In some cases acetic acid was produced almost exclusively, whilst in others the fatty acids consisted of butyric and formic acids, with but traces of acetic acid. The findings in this regard apparently afford no clue to the manner in which cellulose is used for fat production, since propionic acid, the only recognized fat-former among the lower normal fatty acids, was not detected.