Hostname: page-component-8448b6f56d-c4f8m Total loading time: 0 Render date: 2024-04-19T01:20:47.331Z Has data issue: false hasContentIssue false
  • English
  • Français

In vitro degradation of amines by rumen micro-organisms

Published online by Cambridge University Press:  27 March 2009

M. Van Os ,
B. Lassalas ,
S. Toillon  and
J. P. Jouany
M. Van Os
Affiliation:
INRACRZV de TheixStation de Recherches sur la Nutrition des Herbivores63122 Saint Genès-ChampanelleFrance
B. Lassalas
Affiliation:
INRACRZV de TheixStation de Recherches sur la Nutrition des Herbivores63122 Saint Genès-ChampanelleFrance
S. Toillon
Affiliation:
INRACRZV de TheixStation de Recherches sur la Nutrition des Herbivores63122 Saint Genès-ChampanelleFrance
J. P. Jouany
Affiliation:
INRACRZV de TheixStation de Recherches sur la Nutrition des Herbivores63122 Saint Genès-ChampanelleFrance
Get access Rights & Permissions [Opens in a new window]

Summary

Degradation of biogenic amines was studied in rumen contents obtained from wether sheep adapted to diets with different levels of biogenic amines: high (H), low (L) and without (W), containing 7·4, 2·4 and 0 g amines/kg dry matter (DM), respectively. To 200 g of the rumen contents (RC), 2 ml of a solution containing a mixture of the biogenic amines: cadaverine (73·5 mmol/1), histamine (45·0 mmol/1), putrescine (830 mmol/1) and tyramine (123·5 mmol/1) were added, followed by a 5 h incubation in vitro. The fermentation pattern in RC derived from H and L differed from that in RC derived from W. This difference was attributed to differences in fermentative properties of silage and hay-based diets in the rumen. The addition of amines increased ammonia production, which was highest in RC from sheep adapted to silage with the highest amine content (diet H). Amines had no influence on gas production. Amine degradation occurred in all types of RC, but the extent depended on adaptation of the rumen microflora, such that 709, 54·2 and 25·3% of the added quantity in RC from H, L and W, respectively, was degraded. Generally, the breakdown of the individual amines was highest for histamine, followed by tyramine, putrescine and cadaverine. Tyramine breakdown was particularly slow in RC from diet W. These results imply that in animals adapted to grass silage with high concentrations of biogenic amines, the accumulation of amines in the rumen will be prevented by an increase in the amine-degrading capacity of the rumen microbes.

Type
Animals
Information
The Journal of Agricultural Science , Volume 125 , Issue 2 , October 1995 , pp. 299 - 305
Copyright
Copyright © Cambridge University Press 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Baile, C. A. & Della-Fera, M. A. (1988). Physiology of control of food intake and regulation of energy balance in dairy cows. In Nutrition and Lactation in the Dairy Cow (Ed. Garnsworthy, P. C.), pp. 251–261. London: Butterworths.CrossRefGoogle Scholar
Chiofalo, V., Dulphy, J. P. & Baumont, R. (1992). Influence of the method of forage conservation on feeding behaviour, intake and characteristics of the reticulorumen content, in sheep fed ad libitum. Reproduction Nutrition Development 32, 377–392.Google Scholar
Dain, J. A., Neal, A. L. & Dougherty, R. W. (1955). The occurrence of histamine and tyramine in rumen ingesta of experimentally over-fed sheep. Journal of Animal Science 14, 930–935.Google Scholar
Gill, M., Thiago, L. R. S. & Buchanan-Smith, J. G. (1987). Intake problems associated with ensiled forages. In Feed Intake by Beef Cattle (Ed. Owens, F. N.), pp. 341–352. Miscellaneous Publication No. 121, Oklahoma State University.Google Scholar
Gomez, L., Bogaert, C., Jouany, J. P. & Lassalas, B. (1991). The influence of lasalocid and cationomycin on nitrogen digestion in sheep: comparison of methods for estimating microbial nitrogen. Canadian Journal of Animal Science 71, 389–399.CrossRefGoogle Scholar
Hole, J. R. (1985). The nutritive value of silage made from Poa pratensis ssp. alpigna and Phleum pratense. II. Lactating dairy cows fed silage made from first cut of Poa pratensis and Phleum pratense. Scientific Reports of the Agricultural University of Norway 64, 1–20.Google Scholar
Joosten, H. M. L. J. (1988). The biogenic amine contents of Dutch cheese and their toxicological significance. Netherlands Milk and Dairy Journal 42, 25–42.Google Scholar
Jouany, J. P. (1982). Volatile fatty acid and alcohol determination in digestive contents, silage juices, bacterial cultures and anaerobic fermenter contents. Science des Aliments 2, 131–144.Google Scholar
Jouany, J. P. & Thivend, P. (1986). In vitro effects of avoparcin on protein degradability and rumen fermentation. Animal Feed Science and Technology 15, 215–229.Google Scholar
Kay, R. N. B. & Sjaastad, Ø. V. (1974). Absorption and catabolism of histamine in sheep. Journal of Physiology 243, 79–99.CrossRefGoogle ScholarPubMed
Křižek, M. (1993). Biogenic amines in silage. 1. The occurrence of biogenic amines in silage. Archives for Animal Nutrition 43, 169–177.Google ScholarPubMed
Mould, F. L., Ørskov, E. R. & Mann, S. O. (1983). Associative effects of mixed feeds. I. Effects of type and level of supplementation and the influence of the rumen fluid pH on cellulolysis in vivo and dry matter digestion of various roughages. Animal Feed Science and Technology 10, 15–30.CrossRefGoogle Scholar
Neumark, H., Bondi, A. & Volcani, R. (1964). Amines, aldehydes and keto-acids in silages and their effect on food intake by ruminants. Journal of the Science of Food and Agriculture 15, 487–492.CrossRefGoogle Scholar
Ohshima, M. & McDonald, P. (1978). A review of the changes in nitrogenous compounds of herbage during ensilage. Journal of the Science of Food and Agriculture 29, 497–505.CrossRefGoogle Scholar
Prins, R. A. (1977). Biochemical activities of gut microorganisms. In Microbial Ecology of the Gut (Eds Clarke, R. T. J. & Bauchop, T.), pp. 73–183. London: Academic Press.Google Scholar
Sas Institute (1987). SAS/STAT, Guide for Personal Computers, Version 6. Cary, NC: Statistical Analysis System Institute Inc.Google Scholar
Scheline, R. R. (1978). Mammalian Metabolism of Plant Xenobiotics. London: Academic Press.Google Scholar
Schlegel, H. G. (1986). General Microbiology, 6th Edn. Cambridge: Cambridge University Press.Google Scholar
Shlomi, E. R., Lankhorst, A. & Prins, R. A. (1978). Methanogenic fermentation of benzoate in an enrichment culture. Microbial Ecology 4, 249–261.Google Scholar
Tamminga, S., Ketelaar, R. & Van Vuuren, A. M. (1991). Degradation of nitrogenous compounds in conserved forages in the rumen of dairy cows. Grass and Forage Science 46, 427–435.CrossRefGoogle Scholar
Therion, J. J., Kistner, A. & Kornelius, J. H. (1982). Effect of pH on growth rates of rumen amylolytic and lactilytic bacteria. Applied and Environmental Microbiology 44, 428–434.CrossRefGoogle ScholarPubMed
Tveit, B., Llngaas, F., Svendsen, M. & Sjaastad, Ø. V. (1992). Etiology of acetonemia in Norwegian cattle. 1. Effect of ketogenic silage, season, energy level, and genetic factors. Journal of Dairy Science 75, 2421–2432.Google Scholar
Van Eenaeme, C., Bienfait, J. M., Lambot, O. & Pondant, A. (1969). Détermination automatique de l'ammoniaque dans le liquide du rumen par la méthode de Berthelot adapté à l'auto-analyseur. Annales de Medicine Veterinaire 7, 419–429.Google Scholar
Van Os, M., Dulphy, J. P. & Baumont, R. (1995). The effect of protein degradation products in grass silages on feed intake and intake behaviour in sheep. British Journal of Nutrition 73, 51–64.Google ScholarPubMed
Wallace, R. J. & Cotta, M. A. (1988). Metabolism of nitrogen-containing compounds. In The Rumen Microbial Ecosystem (Ed. Hobson, P. N.), pp. 217–249. London: Elsevier Scientific Publishers.Google Scholar